Neuropathology and Optical Imaging Core

神经病理学和光学成像核心

• 批准号: 7805433
• 负责人: Howard Brent Clark
• 金额: $ 20.61万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7805433
• 关键词: Architecture; Area; Arts; Autopsy; Biochemical; Brain; Cerebellar cortex structure; DNA; Data; Functional disorder; Human; Image; In Situ; Investigation; Knowledge; Measures; Methods; Modeling; Molecular; Mus; Myotonic Dystrophy; Nerve Degeneration; Neuraxis; Neuroanatomy; Neurodegenerative Disorders; Organ; Pathology; Patients; Qualifying; RNA; Role; Specimen; Study Subject; System; Time; Tissues; Transgenic Animals; experience; human disease; imaging modality; in vivo; innovation; neuropathology; optical imaging; programs

Scientific Core (Core Leaders, Clark and Ebner) will provide neuropathological, neuroanatomical and in vivo optical imaging expertise required for each project and for the overall Program. Dr. Clark is uniquely qualified for this role, given his many years of experience investigating neurodegenerative disorders using both human autopsy specimens and murine models of human disease. Specifically, the core will investigate the overall brain architecture, specific areas of degeneration, and specific cellular changes in autopsy specimens from DM subjects studied or contacted through Project 3, from the CCUG- and Mbnlloverexpression murine models of Project 1, and from the Mtm/lAE3/AE3 and Mbnll-'- mice of Project 2, using routine histological, immunohistological, and RNA or DNA fluorescent in-situ histological methods. Dr. Clark's extensive experience with both human and murine neurodegeneration, and his knowledge of human and murine neuroanatomy, will help in correlating the imaging and pathological findings, and in focusing investigations in all three Projects. Dr. Tim Ebner, an innovative neuroscienctist and cerebellar physiologist has developed a state of the art, system for optically measuring cerebellar circuits, in vivo. The preliminary data presented in this application are significant not only for their potential to inform us on the pathology of DM but also because Dr. Ebner's imaging methods for the first time allow studies of LTP in vivo. The specific specific focus of the core will be: Focus 1 ¿ The Core will provide neuropathological characterization of patients who have died with myotonic dystrophy types 1 and 2 (DM1 and DM2); Focus 2 ~ The Core will provide neuropathological characterization of central nervous system pathological changes in transgenic animals used to model different aspects of myotonic dystrophies; Focus 3 ¿ The Core will procure and preserve tissue for biochemical studies from CNS and other organs of DM1 and DM2 subjects undergoing autopsy examinations; Focus 4 ¿ The Core will provide the central facilities and expertise for functional in vivo optical imaging of the cerebellar cortex for the murine models of myotonic dystrophy.

科学核心（核心领导者克拉克和艾伯纳）将提供神经病理学、神经解剖学和 每个项目和整个计划所需的 vivo 光学成像专业知识都是独一无二的。 鉴于他多年使用神经退行性疾病研究的经验，他有资格担任这一角色 具体来说，核心将研究人类尸检标本和人类疾病的小鼠模型。 尸检中的整体大脑结构、特定退化区域和特定细胞变化 通过项目 3 研究或接触的 DM 受试者的标本，来自 CCUG- 和 Mbnlloverexpress 项目 1 的小鼠模型以及项目 2 的 Mtm/lAE3/AE3 和 Mbnll-'- 小鼠，使用 常规组织学、免疫组织学和 RNA 或 DNA 荧光原位组织学方法。 克拉克在人类和小鼠神经退行性疾病方面拥有丰富的经验，以及他对人类的了解 和小鼠神经解剖学，将有助于关联成像和病理结果，并聚焦 Tim Ebner 博士是一位创新神经科学家和小脑生理学家。 开发了一种最先进的体内光学测量小脑回路的系统。 本申请中提供的数据具有重要意义，不仅因为它们有可能为我们提供有关病理学的信息 DM 还因为 Ebner 博士的成像方法首次允许在体内研究 LTP 的特异性。 核心的具体焦点将是：焦点 1 ¿核心将提供神经病理学特征 死于 1 型和 2 型强直性肌营养不良（DM1 和 DM2）的患者；焦点 2 ~ The Core 将 提供转基因中枢神经系统病理变化的神经病理学特征 用于模拟强直性肌营养不良的不同方面的动物；焦点 3 ¿核心将采购和 保存 DM1 和 DM2 受试者的中枢神经系统和其他器官的组织用于生化研究 尸检检查；焦点 4 ¿核心区将为职能部门提供中央设施和专业知识 强直性肌营养不良小鼠模型的小脑皮质体内光学成像。