Biospecimen-Core

生物样本核心

基本信息

批准号：
10682550
负责人：
SAYEED IKRAMUDDIN
金额：
$ 20.41万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-30 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10682550
关键词：
Adipocytes Adipose tissue Age Attenuated Automobile Driving Benign Bile fluid Biological Biology Blood Body Weight decreased CDKN2A gene Cells Chemistry Chronic Disease Clinic Collection Confidentiality of Patient Information Consent County Data Data Analyses Department chair Development Disease Ensure Epidemiology Ethics Ethnic Origin Fatty Liver Freezing Fresh Tissue Funding Gender Goals Grant Health Health system Hepatocyte Hospitals Human Individual Infertility Inflammation Insulin Resistance Intervention Studies Laboratories Lesion Liver Longevity Mammals Maps Measures Medical Records Medicine Meta-Analysis Metabolic Metabolic syndrome Metabolism Minnesota Morbidity - disease rate Mus Muscle Operative Surgical Procedures Organ Organ Donor Ovarian Tissue Ovary Participant Pathology Patient Care Patient Recruitments Patients Peripheral Blood Mononuclear Cell Pharmaceutical Preparations Process Protocols documentation Quality Control Race Research Research Personnel Role Sampling Secure Serum Site Skeletal Muscle Socioeconomic Status Specimen Stress Therapeutic Tissue Banks Tissue Sample Tissues Transplantation Tumor Suppressor Proteins age related aging population bariatric surgery biobank biological specimen archives cell type ethical, legal, and social implication ethnic diversity fatty liver disease frailty healthy aging human tissue improved intravenous glucose tolerance test legal implication model organism mortality mouse model participant enrollment peripheral blood pharmacologic professor prospective quality assurance recruit research study response sample collection senescence skeletal social implication socioeconomics spatiotemporal stem cells tissue mapping wound healing

项目摘要

Project Summary Senescent cells (SnCs) accumulate with age and in various chronic disease states and clearly contribute to driving morbidity and mortality in model organisms. Drugs that selectively eliminate SnCs (“senolytics”) were first developed by our team and have been demonstrated to reduce frailty, extend median lifespan, and attenuate morbidity in murine models of numerous age-related diseases. However, SnCs also play a role in wound healing and tissue remodeling. To advance senolytics to their greatest potential, then, it is imperative to understand more about SnCs in humans with healthy aging. As part of SenNet, the proposed Minnesota Tissue Mapping Center (MN TMC) has selected four tissues for identification, characterization, and spatio-temporal analysis of senescence: adipose, skeletal muscle, liver, and ovarian tissues. These tissues were selected because of the scientific expertise at UMN and Mayo Clinic in the biology of these organs, evidence of increased senescence with age, and availability of biobanked specimens as well as surgical access to fresh tissues from healthy individuals. The overall goal of the Biospecimen Core (BSP), co-directed by Dr. Sayeed Ikramuddin, Chair of the UMN Department of Surgery, and Dr. Oyedele Adeyi, Professor of Laboratory Medicine and Pathology, is to provide the tissues and blood for PBMC isolation to the Biological Analysis Core (BAC) for bulk, single cell, and spatial analysis of human SnCs for subsequent multi-plex analysis of the results by the Data Analysis Core (DAC). The BSP will leverage their strong track record of obtaining high-quality, well-annotated human samples from non-chronically diseased individuals who are metabolically characterized and with appropriate diversity in gender, race, socio-economic status, ethnicity, and other relevant diversity parameters. The BSP will also provide access to UMN’s specimen procurement network (BioNet) that has ~70,000 archival tissues (frozen, FFPE) and biofluids (frozen) collected for research, including several adipose depots, several muscles, liver from organ donors, and healthy ovarian tissues removed because of benign lesions. In addition, the BSP has access to tissues within the Rochester Epidemiology Project, which encompasses the medical records of all citizens living in Olmsted County, MN since 1966, along with archived biological specimens. The Aims of the BSP are to: 1) Prepare and maintain all regulatory materials supporting human tissue collection; 2) Recruit and consent patients of different ages for this project; 3) Characterize the metabolic health of enrolled participants; 4) Coordinate with BioNet for intraoperative specimen collection, annotation, and storage of tissues; 5) Procure and annotate high quality target tissues along with relevant biofluids; 6) Longitudinally follow patients for which weight loss and bariatric surgery are metabolic perturbations; 7) Disseminate the samples to MN TMC BAC; and 8) Partner with SenNet to share tissues and to develop shared patient consent and tissue collection protocols with appropriate Ethical, Legal and Social Implications and quality control and assurance considerations.

项目概要衰老细胞 (SnC) 随着年龄的增长和各种慢性疾病状态而积累，并且明显有助于首先是选择性消除 SnCs 的药物（“senolytics”）。由我们的团队开发，并已被证明可以减少虚弱，延长中位寿命，并减轻然而，SnCs 在多种与年龄相关的疾病的小鼠模型中也有一定的发病率。因此，为了发挥 senolytics 的最大潜力，必须了解更多。关于健康衰老的人类中的 SnC 作为拟议的明尼苏达组织绘图中心 SenNet 的一部分。 (MN TMC) 选择了四种组织进行鉴定、表征和时空分析衰老：脂肪、骨骼肌、肝脏和卵巢组织是由于以下原因而被选择的。 UMN 和 Mayo Clinic 在这些器官生物学方面的科学专业知识，衰老加剧的证据随着年龄、生物库样本的可用性以及通过手术获取健康的新鲜组织生物样本核心 (BSP) 的总体目标，由主席 Sayeed Ikramuddin 博士共同领导。 UMN 外科系和检验医学和病理学教授 Oyedele Adeyi 博士为生物分析核心 (BAC) 提供用于分离 PBMC 的组织和血液，用于批量、单细胞、对人类 SnC 进行空间分析，以便数据分析核心对结果进行后续多重分析 (DAC) 将利用其强大的优势。获得高质量、注释清楚的人体样本的记录来自具有代谢特征且具有适当多样性的非慢性病个体菲律宾央行还将性别、种族、社会经济地位、民族和其他相关的多样性参数。提供访问 UMN 的标本采购网络 (BioNet) 拥有约 70,000 个档案组织（冷冻、 FFPE）和生物流体（冷冻）收集用于研究，包括几个脂肪库、一些肌肉、肝脏此外，BSP 还可以访问器官捐献者和因良性病变而被切除的健康卵巢组织。罗切斯特流行病学项目内的组织，其中包含所有公民的医疗记录自 1966 年以来一直居住在明尼苏达州奥姆斯特德县，并存档生物标本。 BSP 的目标是。 1) 准备和维护所有支持人体组织采集的监管材料 2) 招募和同意；该项目不同年龄的患者；3) 描述登记参与者的代谢健康状况；与BioNet协调进行术中标本采集、注释和储存；5）采购和保存；注释高质量目标组织以及相关生物流体；6) 纵向跟踪体重的患者；损失和减肥手术属于代谢紊乱；7) 将样本分发给 MN TMC BAC；以及 8) 与 SenNet 合作，共享组织并制定共享患者同意书和组织采集协议适当的道德、法律和社会影响以及质量控制和保证考虑因素。