COMPARATIVE GENOMICS OF PARASITIC NEMATODES

寄生线虫的比较基因组学

基本信息

批准号：
7898827
负责人：
Makedonka Mitreva
金额：
$ 31.14万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-23 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7898827
关键词：
Active Sites Amino Acid Sequence Anthelmintics Automobile Driving Basic Science Binding Biochemical Pathway Bioinformatics Biological Buffers C. elegans genome Cellular biology Classification Communities Complex Computer Simulation Data Data Analyses Data Set Databases Development Diagnostic Drug Delivery Systems Endocrine system Enzymes Evolution Expressed Sequence Tags Genes Genome Genomics Goals Homologous Gene Hookworms Human Informatics Information Dissemination Intervention Ions Lead Metabolic Metabolic Pathway Metabolism Molecular Morbidity - disease rate National Human Genome Research Institute Nature Nematoda Outcome Parasite Control Parasites Parasitic nematode Parasitology Pathway interactions Peptide Sequence Determination Pharmaceutical Preparations Phosphorylation Site Population Post-Translational Protein Processing Process Protein Family Proteins Reaction Reagent Research Resistance Resources Running Screening procedure Secure Signal Pathway Staging Subcategory Surveys Taxon Testing Therapeutic Time Tissues Toxic effect Universities Vaccine Antigen Vaccines Washington base comparative genomics empowered filaria genome sequencing genome-wide human morbidity improved information processing insertion/deletion mutation model organisms databases mortality novel novel diagnostics parasite genome pathogen programs public health relevance research and development tool

项目摘要

DESCRIPTION (provided by applicant): Parasitic nematodes infect over half the world's population, resulting in significant morbidity and mortality. Characterization of nematode genomes provides fundamental molecular information about these parasites accelerating basic research and development of new diagnostics and therapeutics. Washington University's Genome Center has generated and made public over 400,000 cDNAs from 30 parasitic species, sequenced 4 genomes to draft coverage with ten more underway including representatives of the major human parasitic groups. The three aims in this proposal analyze the expanding nematode sequences to substantially improve understanding of parasitic nematode biology and cellular pathways. First, we will develop and use bioinformatic tools to process, assemble, and annotate incoming data from all sequencing platforms. These genomic resources will also be disseminated to the wider research community through the centralized parasitic nematode database, Nematode.net. Second, analysis will focus on biochemical pathways conserved and/or taxonomically restricted including proteins that may prove useful as drug targets. Third, we will study the nature and implications of nematode-specific insertions and deletions in proteins involved in environmental information processing and endocrine system. The expected outcome will facilitate and promote the discovery and development of novel interventions to control these important parasites and reduced their associated morbidity and mortality. PUBLIC HEALTH RELEVANCE: The continued development of molecular information, bioinformatics tools, and reagents for the study of parasitic nematodes is crucial, as they infect over half of the world's population and are a leading cause of human morbidity. The main goal of this project is to implement comparative genomics approaches to study the biology and cellular pathways of these important parasites, which on a long run will contribute to improved diagnostics, vaccines, and anthelmintic drugs for broad parasite control.

描述（由申请人提供）：寄生线虫感染世界上一半以上的人口，导致显着的发病率和死亡率。线虫基因组的表征提供了有关这些寄生虫的基本分子信息，加速了新诊断和治疗方法的基础研究和开发。华盛顿大学基因组中心已经生成并公开了来自 30 个寄生虫物种的 400,000 多个 cDNA，对 4 个基因组进行了测序，以起草覆盖范围，还有 10 个基因组正在进行中，其中包括主要人类寄生虫群体的代表。该提案的三个目标是分析扩展的线虫序列，以大幅提高对寄生线虫生物学和细胞途径的理解。首先，我们将开发和使用生物信息学工具来处理、组装和注释来自所有测序平台的传入数据。这些基因组资源还将通过集中的寄生线虫数据库 Nematode.net 传播给更广泛的研究界。其次，分析将集中于保守和/或分类学限制的生化途径，包括可能被证明可用作药物靶标的蛋白质。第三，我们将研究涉及环境信息处理和内分泌系统的蛋白质中线虫特异性插入和缺失的性质和影响。预期结果将促进和促进新干预措施的发现和开发，以控制这些重要的寄生虫并降低其相关的发病率和死亡率。公共卫生相关性：用于研究寄生线虫的分子信息、生物信息学工具和试剂的持续开发至关重要，因为它们感染了世界一半以上的人口，并且是人类发病的主要原因。该项目的主要目标是采用比较基因组学方法来研究这些重要寄生虫的生物学和细胞途径，从长远来看，这将有助于改进诊断、疫苗和驱虫药物，以实现广泛的寄生虫控制。