GENOME-WIDE VIEW OF REPLICATION FORK PROGRESSION AND ARREST

复制叉进展和停滞的全基因组视图

• 批准号: 8361545
• 负责人: Brian T Chait
• 金额: $ 2.61万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361545
• 关键词: Affect; Architecture; Cell Cycle; Chromatin Structure; Complex; Coupled; DNA Polymerase III; DNA biosynthesis; Data; Doctor of Medicine; Eukaryota; Funding; Fungal Genome; Genome; Genomics; Grant; Manuscripts; Monitor; Motion; National Center for Research Resources; Principal Investigator; Publishing; Research; Research Infrastructure; Resources; Saccharomycetales; Site; Source; System; United States National Institutes of Health; Work; Yeasts; cost; genome-wide; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA replication in eukaryotes has been extensively studied at the genome wide level in budding yeast. These studies have led to a picture wherein DNA replication initiates from a large number of origins and progresses at widely variable rates over different parts of the genome. The progressing fork has also been described to pause at numerous specific sites in the genome of wild type yeast. These data have been interpreted to mean that the dynamics of replication fork progression are strongly affected by local chromatin structure or architecture. Here, we monitored the genomic association of the replication fork-coupled GINS complex through the cell cycle. A manuscript describing this work has been published: M.D. Sekedat, D. Feny¿, R.S. Rogers, A.J. Tackett, J.D. Aitchison, B.T. Chait "GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome" Mol Systems Biol, 6 (2010) 353 We are currently extending this work to the study of the dynamics of the epsilon and delta DNA polymerases

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 真核生物中的DNA复制已广泛研究在萌芽酵母中的基因组宽水水平。这些研究导致了一张图片，其中DNA复制从大量起源开始，并且在基因组的不同部分以广泛的速率进展。还描述了进步的叉子在野生型酵母基因组中的许多特定位点停顿。这些数据被解释为意味着复制叉进程的动力学受到局部染色质结构或体系结构的强烈影响。在这里，我们监测了整个细胞周期复制叉耦合循环复合物的基因组关联。描述这项工作的手稿已经发表： M.D. Sekedat，D。Feny¿，R.S。罗杰斯，A.J。塔克特（J.D. Aitchison） Chait“杜松子运动揭示了整个酵母基因组的复制叉进展非常均匀” Mol Systems Biol，6（2010）353 我们目前正在将这项工作扩展到研究Epsilon和Delta DNA聚合酶的动力学