Neuronal Physiology Core

神经生理学核心

• 批准号: 10667318
• 负责人: Paulette B. Goforth
• 金额: $ 39.86万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10667318
• 关键词: Advisory Committees; Animals; Biological Assay; Body Weight; Brain; Calcium; Calcium Signaling; Cell Culture Techniques; Cell model; Cell physiology; Cells; Cellular Assay; Collaborations; Communication; Complement; Computer software; Consultations; Custom; Data; Data Analyses; Data Collection; Doctor of Philosophy; Electrophysiology (science); Equipment; Experimental Designs; Feeding behaviors; Fiber; Food Intake Regulation; Gastrectomy; Gastrointestinal tract structure; Genetic; Genetic Models; Human Resources; Hypothalamic structure; Image; Imaging Techniques; Individual; Ions; Label; Laboratories; Measurement; Measures; Metabolic Diseases; Methodology; Michigan; Monitor; Neurons; Noise; Output; Photometry; Physiological; Physiological Processes; Physiology; Play; Population; Postdoctoral Fellow; Preparation; Process; Protocols documentation; Quality Control; Regulation; Reproducibility; Research Design; Research Project Grants; Rodent; Role; Sample Size; Secure; Services; Slice; Standardization; Stimulus; Structure; Students; Supervision; Techniques; Technology; Testing; Training; Universities; Visualization; Work; bariatric surgery; calcium indicator; conditioned place preference; electrical measurement; experience; experimental study; extracellular; fluorophore; gut-brain axis; hindbrain; in vivo; interest; meetings; mouse model; neural; neural circuit; neuronal circuitry; novel; operation; optogenetics; patch clamp; programs; response; theories; tool

Neuronal Physiology Core Abstract: The studies proposed in this Program Project will define the structure, function and regulation of hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that occur within this circuitry in response to bariatric surgery. To probe the function and regulation of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology Core will conduct specialized assays of cellular function that are targeted to select neuronal subtypes using genetically encoded tools combined with unique genetic mouse models. These assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically targeted neuronal populations to assess the impact of their modulation on physiologic processes (e.g. feeding behavior, conditioned place preference)

神经生理学核心 抽象的： 本计划项目中提出的研究将定义结构、功能和监管 后脑神经回路是肠脑通讯的基础，并确定 发生在该电路内以响应减肥手术。探究功能和调节 在细胞水平上，该电路内不同的神经元群，神经元生理学 Core 将进行专门的细胞功能分析，以选择神经元 使用基因编码工具结合独特的基因小鼠模型来识别亚型。这些 测定包括 1. 体内光纤光度测定法，测量基因编码的钙信号 钙指示剂（GECI）表达后脑神经元群，作为细胞的读数 VSG 前后对刺激的反应活动。 2. 离体电生理分析 基因标记和鉴定的神经元可直接评估潜在的细胞机制 垂直袖状胃切除术 (VSG) 3.combined 前后的功能和调节 光遗传学和离体电生理学来评估定义的功能连接 4. 体内光遗传学刺激或抑制不同遗传的神经元群体 针对神经元群体来评估其调节对生理的影响 过程（例如进食行为、条件性位置偏好）