DIRECT SAMPLE ANALYSIS WITH AN ATMOSPHERIC PRESSURE GLOW DISCHARGE

利用大气压辉光放电进行直接样品分析

基本信息

批准号：
7611686
负责人：
Justin Michael Wiseman
金额：
$ 10.2万
依托单位：
PROSOLIA, INC.
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-09 至 2010-02-08
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7611686
关键词：
Ablation Acute Address Air Anodes Area Atmospheric Pressure Biochemical Biological Biological Assay Biological Sciences Biomedical Research Cathodes Cells Cellular Structures Characteristics Charge Chemicals Clinical Coupling Cytochrome P450 Detection Development Diffuse Electrodes Electrons Electrospray Ionization Environment Hand IACUC Image In Vitro Indiana Ions Laboratories Lasers Lateral Letters Libraries Marketing Mass Spectrum Analysis Measurement Measures Medical Methods Modification Nature Nephrology Neurology Performance Pharmacologic Substance Phase Plasma Preparation Procedures Reagent Research Ethics Committees Resolution Sampling Scheme Screening procedure Small Business Technology Transfer Research Source Spatial Distribution Spottings Structure Surface System Techniques Technology Temperature Time Tissues Universities base combinatorial commercialization density design high throughput screening in vivo innovation instrumentation interest ion mobility ion source ionization ionization technique mass spectrometer medical schools molecular imaging new technology oncology planetary Atmosphere pre-clinical prototype public health relevance tool

项目摘要

DESCRIPTION (provided by applicant): The overall objective of this Phase I STTR project is the commercialization of a new ionization source for ambient mass spectrometry based on the flowing afterglow of an atmospheric pressure glow discharge (APGD). This technology promises to have significant impact in pharmaceutical, clinical and biomedical research and its potential for enabling in vivo mass spectrometry detection in combination with existing technology is real. There is an acute need for the development of new technologies which enable rapid measurements with minimal sample pretreatment. Minimizing up-front sample preparation increases sample through-put by reducing the time required to go from raw material to a result. The recent development of ambient mass spectrometry methods has already resulted in the successful commercialization of just a few of these disruptive technologies. This project involves the fundamental characterization, optimization and development of a new direct sampling technology for mass spectrometry based on the flowing afterglow of an atmospheric pressure glow discharge developed at Indiana University by Prof. Gary Heiftje. Atmospheric pressure glow discharges have been extensively studied but not until very recently has it been attempted to use an atmospheric pressure glow discharge for direct sampling of surfaces in the open ambient air. We envision commercial ion sources that can be easily converted among several atmospheric pressure ionization techniques and can be retro-fitted to several different types of mass spectrometers or ion mobility spectrometers. The specific aims of this Phase I STTR proposal are: Aim 1: Investigate the APGD and flowing afterglow chemical environment using spectroscopic techniques. Aim 2: Identify electrode structures and materials for the anode and cathode in an attempt to optimize the atmospheric glow discharge characteristics using the information gained through completion of Aim 1. An "optimized" cell will be one which maximizes reagent ion density and controls their spatial distribution. Aim 3: Operate the APGD cell in combination with mass spectrometry and identify operating conditions for the cell geometries investigated in Aim 2. Aim 4: Assess the feasibility for coupling APGD to a commercial laser ablation mass spectrometry system. Phase II of this project will include the development of a commercial prototype of the APGD ion source based on the criteria defined in Phase I, further optimization of its performance and robustness, comparisons to other ambient ionization methods and applications development in the areas of pharmaceutical and biomedical research. Specifically, Phase II will focus in part on the development of the APGD cell in combination with commercially available laser ablation mass spectrometry systems, which will enable direct, 3D molecular imaging of biological tissues. Other application areas that will be explored with the same instrumentation include high throughput screening of combinatorial libraries and in vitro cytochrome P450 assays. PUBLIC HEALTH RELEVANCE: Prosolia's new and versatile ambient ionization source for mass spectrometry promises to enable high throughput chemical screening that will significantly impact pharmaceutical, clinical and biomedical research.

描述（由申请人提供）：第一阶段 STTR 项目的总体目标是基于大气压辉光放电 (APGD) 的流动余辉，实现用于环境质谱分析的新型电离源的商业化。该技术有望对制药、临床和生物医学研究产生重大影响，并且其与现有技术相结合实现体内质谱检测的潜力是真实的。迫切需要开发新技术，以最少的样品预处理实现快速测量。最大限度地减少前期样品制备，减少从原材料到结果所需的时间，从而提高样品通量。环境质谱方法的最新发展已经导致其中一些颠覆性技术成功商业化。该项目涉及一种新的质谱直接采样技术的基本表征、优化和开发，该技术基于印第安纳大学 Gary Heiftje 教授开发的大气压辉光放电的流动余辉。大气压辉光放电已被广泛研究，但直到最近才尝试使用大气压辉光放电对开放环境空气中的表面进行直接采样。我们设想商业离子源可以在几种大气压电离技术之间轻松转换，并且可以改装到几种不同类型的质谱仪或离子迁移谱仪。第一阶段 STTR 提案的具体目标是：目标 1：使用光谱技术研究 APGD 和流动余辉化学环境。目标 2：确定阳极和阴极的电极结构和材料，以尝试使用通过完成目标 1 获得的信息来优化大气辉光放电特性。“优化”电池将是一种最大化试剂离子密度并控制其空间的电池分配。目标 3：结合质谱分析操作 APGD 池，并确定目标 2 中研究的细胞几何形状的操作条件。目标 4：评估将 APGD 耦合到商用激光烧蚀质谱系统的可行性。该项目的第二阶段将包括根据第一阶段定义的标准开发 APGD 离子源的商业原型，进一步优化其性能和稳健性，与其他环境电离方法进行比较以及制药和制药领域的应用开发。生物医学研究。具体来说，第二阶段将部分侧重于与市售激光烧蚀质谱系统相结合的 APGD 细胞的开发，这将实现生物组织的直接 3D 分子成像。将使用相同仪器探索的其他应用领域包括组合文库的高通量筛选和体外细胞色素 P450 测定。公共健康相关性：Prosolia 用于质谱分析的新型多功能环境电离源有望实现高通量化学筛选，这将显着影响制药、临床和生物医学研究。