DIRECT SAMPLE ANALYSIS WITH AN ATMOSPHERIC PRESSURE GLOW DISCHARGE

利用大气压辉光放电进行直接样品分析

基本信息

批准号：
7611686
负责人：
Justin Michael Wiseman
金额：
$ 10.2万
依托单位：
PROSOLIA, INC.
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-09 至 2010-02-08
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7611686
关键词：
Ablation Acute Address Air Anodes Area Atmospheric Pressure Biochemical Biological Biological Assay Biological Sciences Biomedical Research Cathodes Cells Cellular Structures Characteristics Charge Chemicals Clinical Coupling Cytochrome P450 Detection Development Diffuse Electrodes Electrons Electrospray Ionization Environment Hand IACUC Image In Vitro Indiana Ions Laboratories Lasers Lateral Letters Libraries Marketing Mass Spectrum Analysis Measurement Measures Medical Methods Modification Nature Nephrology Neurology Performance Pharmacologic Substance Phase Plasma Preparation Procedures Reagent Research Ethics Committees Resolution Sampling Scheme Screening procedure Small Business Technology Transfer Research Source Spatial Distribution Spottings Structure Surface System Techniques Technology Temperature Time Tissues Universities base combinatorial commercialization density design high throughput screening in vivo innovation instrumentation interest ion mobility ion source ionization ionization technique mass spectrometer medical schools molecular imaging new technology oncology planetary Atmosphere pre-clinical prototype public health relevance tool

项目摘要

DESCRIPTION (provided by applicant): The overall objective of this Phase I STTR project is the commercialization of a new ionization source for ambient mass spectrometry based on the flowing afterglow of an atmospheric pressure glow discharge (APGD). This technology promises to have significant impact in pharmaceutical, clinical and biomedical research and its potential for enabling in vivo mass spectrometry detection in combination with existing technology is real. There is an acute need for the development of new technologies which enable rapid measurements with minimal sample pretreatment. Minimizing up-front sample preparation increases sample through-put by reducing the time required to go from raw material to a result. The recent development of ambient mass spectrometry methods has already resulted in the successful commercialization of just a few of these disruptive technologies. This project involves the fundamental characterization, optimization and development of a new direct sampling technology for mass spectrometry based on the flowing afterglow of an atmospheric pressure glow discharge developed at Indiana University by Prof. Gary Heiftje. Atmospheric pressure glow discharges have been extensively studied but not until very recently has it been attempted to use an atmospheric pressure glow discharge for direct sampling of surfaces in the open ambient air. We envision commercial ion sources that can be easily converted among several atmospheric pressure ionization techniques and can be retro-fitted to several different types of mass spectrometers or ion mobility spectrometers. The specific aims of this Phase I STTR proposal are: Aim 1: Investigate the APGD and flowing afterglow chemical environment using spectroscopic techniques. Aim 2: Identify electrode structures and materials for the anode and cathode in an attempt to optimize the atmospheric glow discharge characteristics using the information gained through completion of Aim 1. An "optimized" cell will be one which maximizes reagent ion density and controls their spatial distribution. Aim 3: Operate the APGD cell in combination with mass spectrometry and identify operating conditions for the cell geometries investigated in Aim 2. Aim 4: Assess the feasibility for coupling APGD to a commercial laser ablation mass spectrometry system. Phase II of this project will include the development of a commercial prototype of the APGD ion source based on the criteria defined in Phase I, further optimization of its performance and robustness, comparisons to other ambient ionization methods and applications development in the areas of pharmaceutical and biomedical research. Specifically, Phase II will focus in part on the development of the APGD cell in combination with commercially available laser ablation mass spectrometry systems, which will enable direct, 3D molecular imaging of biological tissues. Other application areas that will be explored with the same instrumentation include high throughput screening of combinatorial libraries and in vitro cytochrome P450 assays. PUBLIC HEALTH RELEVANCE: Prosolia's new and versatile ambient ionization source for mass spectrometry promises to enable high throughput chemical screening that will significantly impact pharmaceutical, clinical and biomedical research.

描述（由申请人提供）：I阶段ISTTR项目的总体目标是基于大气压力释放（APGD）的流动余辉的环境质谱新的电离源的商业化。这项技术有望在药物，临床和生物医学研究中产生重大影响，及其在与现有技术结合使用体内质谱检测的潜力是真实的。急需开发新技术，可以通过最少的样品预处理进行快速测量。通过减少从原材料到结果所需的时间来最大程度地减少前期样品制备，从而增加了样本。环境质谱法的最新发展已经导致了仅一些破坏性技术的成功商业化。该项目涉及基于Gary Heiftje教授在印第安纳大学开发的大气压力发光的流动余辉的基本表征，优化和开发用于质谱的新直接采样技术。大气压力发光的放电已经进行了广泛的研究，但直到最近才尝试使用大气压力放电来直接对开放环境空气中的表面进行直接采样。我们设想可以在几种大气压力电离技术中轻松转换的商业离子源，并且可以重新安装到几种不同类型的质谱仪或离子迁移率光谱仪上。该阶段ISTTR建议的具体目的是：目标1：使用光谱技术研究APGD和流动的余辉环境。 AIM 2：通过通过完成AIM 1获得的信息来优化阳极和阴极的电极结构和材料，以优化大气发光放电特性。“优化”的细胞将是最大化试剂离子密度并控制其空间分布的细胞。 AIM 3：与质谱结合使用APGD细胞，并确定AIM 2中研究的细胞几何形状的工作条件。AIM4：评估将APGD耦合到商业激光消融质谱系统的可行性。该项目的第二阶段将包括基于第一阶段定义的标准，其性能和鲁棒性的进一步优化的APGD离子源的商业原型，与其他环境电离方法的比较以及在药物和生物医学研究领域中的其他环境电离方法的开发。具体而言，第二阶段将重点关注APGD细胞的开发以及市售激光消融质谱系统，该系统将启用生物组织的直接3D分子成像。将使用相同仪器探索的其他应用区域包括组合库的高吞吐量筛选和体外细胞色素P450分析。公共卫生相关性：Prosolia的质谱法新的和多功能的环境电离源有望实现高吞吐性化学筛查，这将显着影响药物，临床和生物医学研究。