Cohort Administration and Biorepository Core

队列管理和生物样本库核心

基本信息

批准号：
10633366
负责人：
Antti Seppo
金额：
$ 23.99万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-07 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10633366
关键词：
6 year old Age Allergens Allergic Allergic Disease Asthma Atopic Dermatitis Biological Markers Birth Child Childhood Clinic Clinical Cohort Studies Communities Consumption County Data Development Diagnosis Disease Epigenetic Process Epithelial Cells Equipment and supply inventories Europe Exposure to Family Farm Feces Fingers First Degree Relative Food Hypersensitivity German population Hay fever Home House Dust Hypersensitivity Immune Immunologic Markers Immunology Impairment Incidence Individual Infant Infant Development Inflammatory Innate Immune System Insurance Life Life Style Livestock Measures Medical Mennonite Metabolic Pathway Metadata Milk North America Outcome Patient Self-Report Permeability Physicians Population Pregnant Women Prevention strategy Primary Prevention Process Production Regulatory T-Lymphocyte Research Personnel Risk Sampling School-Age Population Secondary Prevention Siblings Signs and Symptoms Skin Surveys Swab TSLP gene Testing Visit Woman atopy biobank biomarker identification clinical development cohort comparison group computerized data processing cytokine data infrastructure early childhood early onset follow-up food allergen gut microbiome high risk infant gut microbiome metabolome microbial microbiome composition neonate novel strategies peripheral blood prenatal programs recruit research clinical testing respiratory sample collection shared database skin barrier symposium unpasteurized urban children

项目摘要

PROJECT SUMMARY/ABSTRACT – COHORT ADMINISTRATION & BIOREPOSITORY CORE A large body of data suggests that living on farms is associated with a decreased risk of asthma and atopic diseases, including studies from Europe and the North America. The individual factors that appear to be associated with this “farm-life effect” include consumption of unpasteurized farm milk and exposure to farm animals and stables. Farm children have higher numbers of regulatory T cells, compared to urban children, and their innate immune system also appears to be the target for these exposures. House dust in farm homes has greater microbial diversity that is strongly negatively associated with the incidence of asthma. The studies thus far have focused on outcomes of asthma at school-age. However, atopic dermatitis and food allergy commonly precede development of respiratory allergies in a so-called “atopic march”. Our studies in the Old Order Mennonite Community have indicated that they are at low risk for atopic diseases and the infant gut microbiome composition is different from Rochester infants. The Cohort Admin and Biorepository Core will form the sample and data infrastructure for the distinct but complementary Projects 1-3 of this U19 Program, “Biomarkers of Atopy Beginning Early” (BABE). The Core will follow up and expand an ongoing longitudinal birth cohort study of OOM neonates at a very low risk for atopic dermatitis, food allergies, hayfever and asthma and Rochester neonates from atopic families with a high risk for developing these atopic manifestations. We will test the overall hypothesis that infant gut microbiome associated metabolic pathways and innate stimulation promote tolerance and barrier function in the OOM. The specific aims of the Core are to Aim 1) sample and clinically follow up already recruited Cohort 1 infants until 6 years of age for additional allergic diseases, Aim 2) recruit, sample and clinically follow up a similar cohort 2 of 120 infants for the development of atopic dermatitis and food allergy until 24mo of age, and Aim 3) prepare, store and share samples utilized in the Projects 1-3 (cord and infant peripheral blood, stool, skin swabs and tape strips) to assess farming lifestyle effect on children's gut microbiome, innate and adaptive immune composition, and skin barrier function and microbiome composition. These studies aim for novel strategies for primary and secondary prevention of early childhood allergic diseases.

项目摘要/摘要 – 队列管理和生物样本库核心大量数据表明，住在农场可以降低患哮喘和特应性过敏的风险疾病，包括来自欧洲和北美的研究似乎是个体因素。与这种“农场生活效应”相关的包括消费未经高温消毒的农场牛奶和接触农场与城市儿童相比，农场儿童的调节性 T 细胞数量更高，并且他们的先天免疫系统似乎也是这些暴露于农舍灰尘的目标。更大的微生物多样性与哮喘的发病率呈强烈负相关。目前，人们关注的是学龄期哮喘的结果，但特应性皮炎和食物过敏也很常见。在所谓的“特应性游行”中，我们的研究遵循旧秩序，先于呼吸道过敏的发展。门诺社区表示，他们患特应性疾病和婴儿肠道微生物组的风险较低成分与罗切斯特婴儿不同。队列管理和生物样本库核心将为不同但该 U19 计划的补充项目 1-3，“特应性早期开始的生物标志物”(BABE)。将跟进并扩大一项正在进行的纵向出生队列研究，对象是特应性风险极低的 OOM 新生儿皮炎、食物过敏、花粉热和哮喘以及来自特应性家庭的罗切斯特新生儿具有高风险我们将检验婴儿肠道微生物组相关的总体假设。代谢途径和先天刺激促进 OOM 中的耐受性和屏障功能。核心目标是 1) 对已招募的第 1 组婴儿进行采样和临床随访直至 6 岁对于其他过敏性疾病，目标 2) 招募 120 名婴儿的类似队列 2，对其进行采样和临床随访预防特应性皮炎和食物过敏直至 24 个月大，目标 3) 准备、储存和分享项目 1-3 中使用的样本（脐带和婴儿外周血、粪便、皮肤拭子和胶带）用于评估农业生活方式对儿童肠道微生物组、先天性和适应性免疫组成以及皮肤屏障的影响功能和微生物组成。这些研究旨在寻找幼儿过敏一级和二级预防的新策略疾病。