Trapped Ion Mobility Spectrometry Quadrupole Time-of-Flight System

俘获离子淌度光谱四极杆飞行时间系统

• 批准号: 10632999
• 负责人: CLAUDIA S MAIER
• 金额: $ 134.57万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-05 至 2024-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632999
• 关键词: 12 year old; Acceleration; Aging; Animals; Biological Models; Biomedical Research; Botanical dietary supplements; Communities; Competence; Core Facility; Employee; Ensure; Environment; Environmental Health; Equipment; Faculty; Fostering; Funding; Health; Human; Innovative Therapy; Institution; Knowledge; Lasers; Maintenance; Mass Spectrum Analysis; Measurement; Metabolic Diseases; Neurodegenerative Disorders; Oregon; Plasma; Policies; Postdoctoral Fellow; Research; Research Project Grants; Research Support; Resolution; Sampling; Science; Source; Spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; System; Time; Tissues; Toxicology; Training; Universities; Vascular Cognitive Impairment; Zebrafish; college; data management; diagnostic biomarker; human disease; imaging capabilities; innovation; instrument; inter-institutional; ion mobility; lipidomics; mass spectrometric imaging; nanoscale; novel diagnostics; operation; programs; promote resilience; small molecule; student training

Project Summary Oregon State University is requesting funds for adding a trapped ion mobility spectrometry quadrupole time-of-flight (timsTOF) LC-MS system with MALDI imaging capability to advance biomedical research at Oregon State University (OSU) and in the State of Oregon. The new instrument will catalyze OSU's “ONE Health” research initiative, promoting collaborative and trans-disciplinary research in human, animal and environmental health to increase our knowledge of the underlying causes of human diseases, contribute to discovery of new diagnostic markers and innovative therapies. The proposed Bruker timsTOF fleX MS system will allow Oregon's research community - for the first time - to conduct MALDI imaging mass spectrometry and spatial “omics” to accelerate progress in ongoing projects related to the “One Health” paradigm. The requested equipment will enhance capabilities in small molecule omics by providing access to high-resolution ion mobility mass spectrometry and CCS determination, enabling compound identification and 4D “omics” (RT, mass, MS2 and CCS). Increased measurement sensitivity and the advanced timsPASEF acquisition mode will enable nano-scale LC-MS based “omics” for amount-limited samples and laser-capture microdissected tissues. An innovative HESI source will advance our capabilities to provide comprehensive plasma lipidomics. The proposed equipment will replace 12-year old qTOF systems in OSU's mass spectrometry center (OSU MSC). The OSU MSC has provided a collaborative research and inclusive training environment since 1973. As university core facility, the OSU MSC has institutional support from the research office and multiple colleges, which ensures a high level of financial and operational stability. The OSU MSC is directed by faculty with indefinite tenure. Two full time employees ensure a high level of technical competency and consistency in the maintenance and operation of the equipment, associated workflows and data management. Research associates provide support to research projects and assist in training of students and users. An internal oversight committee will coordinate use of the requested equipment and administration of policies. Research topics include metabolic diseases, vascular cognitive impairment and neurodegenerative diseases, botanical supplements to promote resilience in aging and zebrafish as whole system model in toxicology. A pilot and feasibility program will promote the new measurement capabilities and MALDI imaging mass spectrometry. The new equipment will enhance statewide efforts of the major research universities, centers and institutions in Oregon to coordinate and foster inter-institutional research and training in the biomedical sciences.

项目概要 俄勒冈州立大学正在申请资金以增加捕获离子淌度光谱仪 具有 MALDI 成像功能的四极杆飞行时间 (timsTOF) LC-MS 系统可推进 俄勒冈州立大学 (OSU) 和俄勒冈州的生物医学研究。 该仪器将促进俄勒冈州立大学的“ONE Health”研究计划，促进协作和 人类、动物和环境健康方面的跨学科研究，以增加我们的知识 人类疾病的根本原因，有助于发现新的诊断标记物 拟议的 Bruker timsTOF fleX MS 系统将使俄勒冈州能够实现这一目标。 研究界首次开展 MALDI 成像质谱和空间分析 “组学”加速与“同一个健康”范式相关的正在进行的项目的进展。 所要求的设备将通过提供访问来增强小分子组学的能力 高分辨率离子淌度质谱和 CCS 测定，使化合物 识别和 4D“组学”（RT、质量、MS2 和 CCS）提高测量灵敏度和 先进的 timsPASEF 采集模式将实现基于纳米级 LC-MS 的“组学” 数量有限的样品和激光捕获显微切割组织将被创新的 HESI 来源。 我们有能力提供全面的血浆先进脂质组学建议的设备。 将取代 OSU 质谱中心 (OSU MSC) 已有 12 年历史的 qTOF 系统。 自 1973 年以来，MSC 一直提供协作研究和包容性培训环境。 大学核心设施，俄勒冈州立大学 MSC 得到了研究办公室和 多个学院，确保了 OSU MSC 的高水平财务和运营稳定性。 由两名全职员工指导，确保高水平的教学。 设备维护和操作的技术能力和一致性， 相关的工作流程和数据管理为研究提供支持。 内部监督委员会将协助培训学生和用户。 协调所要求的设备的使用和政策的管理，包括。 代谢疾病、血管性认知障碍和神经退行性疾病、植物学 促进衰老的补充剂和斑马鱼作为毒理学的整个系统模型。 试点和可行性计划将推广新的测量功能和 MALDI 成像 新设备将加强重大研究的宣讲力度。 俄勒冈州的大学、中心和机构协调和促进机构间研究 和生物医学科学培训。