Cryo-TEM for Biomedical Research

用于生物医学研究的冷冻透射电镜

• 批准号: 10629910
• 负责人: ROBBERT J CRETON
• 金额: $ 139.08万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10629910
• 关键词: Advisory Committees; Aging; Antibiotic Resistance; Area; Biochemical; Biochemical Process; Biology; Biomedical Research; Communities; Cryoelectron Microscopy; Data Analyses; Data Storage and Retrieval; Disease; Electron Microscope; Equipment; Evaluation; Funding; Grant; Health; Image; Infertility; Institution; Left; Medicine; Microscope; Minor; Neurodegenerative Disorders; Organelles; Protein Dynamics; Research Personnel; Research Project Grants; Resolution; Rhode Island; Sampling; Technical Expertise; Technology; Tissues; Training; Travel; United States National Institutes of Health; Universities; Vesicle; Visualization; bioimaging; imaging facilities; imaging system; instrument; instrumentation; neural; particle; protein structure; structural biology; transmission process

Summary Brown University requests support for a Tundra Cryo-Transmission Electron Microscope (Cryo-TEM). This microscope will be used to examine protein structure by single particle analysis for a better basic understanding of biochemical processes in health and disease. Vitrified samples imaged on the Tundra Cryo-TEM can be recovered for cryo-electron microscopy (cryo-EM) at high resolution in national cryo-EM centers. In addition, the requested microscope will be used as a standard TEM for imaging small vesicles, organelles and neural connections in ultrathin tissue sections. The Tundra Cryo-TEM will replace a 39-year-old TEM in the Division of Biology and Medicine and will be the first Cryo-TEM capable of single particle analysis in the State of Rhode Island. The Cryo-TEM will be used immediately by 16 investigators. Collectively, these 16 user groups are funded by 31 NIH grants, covering a broad area in biomedical research ranging from antibiotic resistance and infertility to aging and neurodegenerative disease. All major users have a substantial need for cryo-EM. These major users either traveled out of state for cryo-EM or used other technologies that left questions unanswered, especially when studying large dynamic proteins. The minor users plan to use the requested microscope as a standard TEM or have cryo-EM projects that were put on hold since the required equipment has not been available at Brown University. All major and minor users have research projects that would immediately benefit from the proposed equipment. The impact of the proposed imaging system will extend beyond the projects of the major and minor users, since the microscope will be made be made available to the broader scientific community in Rhode Island, which has over 700 active NIH-funded projects. The microscope will be set up in the Leduc Bioimaging Facility, which has the technical expertise to maintain the instrument and train its users. In addition, biochemical sample optimization and evaluation will be supported by Brown University’s Structural Biology Facility and data storage and analysis will be supported by the Center for Computation and Visualization. The Leduc Bioimaging Facility has a broadly-based Advisory Committee, an excellent track record maintaining various high-end imaging systems, and strong institutional support, which guarantees that the instrumentation will be used to its maximum potential for years to come.

概括 布朗大学请求支持 Tundra 冷冻透射电子显微镜 (Cryo-TEM)。 显微镜将用于通过单颗粒分析检查蛋白质结构，以获得更好的基本了解 在 Tundra Cryo-TEM 上成像的玻璃化样品可以分析健康和疾病中的生化过程。 在国家冷冻电镜中心以高分辨率进行冷冻电子显微镜 (cryo-EM) 恢复。 所要求的显微镜将用作小囊泡、细胞器和神经成像的标准 TEM Tundra Cryo-TEM 将取代该部门已有 39 年历史的 TEM。 生物学和医学，将成为罗德州第一个能够进行单粒子分析的冷冻电镜 Island. 冷冻电镜将立即由 16 名研究人员使用，这 16 个用户组总共得到资助。 获得 31 项 NIH 资助，涵盖生物医学研究的广泛领域，从抗生素耐药性到不孕不育 所有主要用户都对冷冻电镜有大量需求。 用户要么出国寻求冷冻电镜，要么使用其他未解答问题的技术， 特别是在研究大型动态蛋白质时，未成年人计划使用所需的显微镜作为显微镜。 标准 TEM 或因所需设备尚未到位而被搁置的冷冻电镜项目 所有主要和次要用户都有可以立即受益的研究项目。 拟议的成像系统的影响将超出该项目的范围。 主要和次要用户，因为显微镜将提供给更广泛的科学界 罗德岛州社区有 700 多个由 NIH 资助的活跃项目。 Leduc 生物成像设施，拥有维护仪器和培训用户的技术专业知识。 此外，生化样品的优化和评估将得到布朗大学结构研究所的支持 生物设施以及数据存储和分析将由计算和可视化中心提供支持。 Leduc 生物成像设施拥有一个基础广泛的咨询委员会，保持着良好的记录 各类高端影像系统，强大的机构支持，保证了仪器 将在未来几年内发挥其最大潜力。