BAC LIBRARY PRODUCTION FOR COMPARATIVE GENETICS

用于比较遗传学的 BAC 文库制作

• 批准号: 7716066
• 负责人: Pieter J. de Jong
• 金额: $ 2.39万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716066
• 关键词: Animal Model; Animals; Arts; Bacterial Artificial Chromosomes; Chromosomes, Human, Pair 1; Cloning; Collection; Communities; Complement; Computer Retrieval of Information on Scientific Projects Database; Economics; Ensure; Evolution; Fingerprint; Funding; Genes; Genetic; Genome; Goals; Grant; Home Page; Institution; Laboratories; Lead; Libraries; Pediatric Hospitals; Preparation; Procedures; Process; Production; Publications; Quality Control; Research; Research Institute; Research Personnel; Resources; Screening procedure; Source; Standards of Weights and Measures; Technology; Testing; United States National Institutes of Health; Work; base; comparative; functional genomics; gene function; human disease; improved; interest; research and development; size; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal is to prepare BAC libraries for many species, of high quality, tailored to the scientific community's interest in comparative genomics and functional applications. The work will be done by two collaborating teams, one lead by Dr. Cheng at the Lawrence Berkeley National Laboratory (LBNL) and the other one at the Children's Hospital Oakland Research Institute, co-directed by Drs. Osoegawa & deJong. The application has four components relevant to preparing Bacterial Artificial Chromosomes (BACs): 1) BAC Library preparation, 2) Clone Arraying & Library Duplication, 3) Library Characterization, and 4) Research & Development to improve the overall process efficiency and the BAC library quality. During the first year, the consortium will generate and characterize twelve animal BAC libraries with ten-fold genome redundancy. Presuming an increasing need for additional GBACs and improved production efficiency, 17 and 22 additional libraries will be prepared and characterized in years 2 and 3 respectively. The libraries, based on current BAC cloning technology, will complement and extend the small repertoire of BAC clone collections now available for comparative genome analysis. The new clone collections will be analyzed by a standardized set of tests, including screening with a set of economic markers, limited BAC-end sequencing, BAC fingerprinting, BAC stability analysis and insert size determination. Of particular importance are quality tests to ensure low BAC stability analysis and insert size determination. Of particular importance are quality tests to ensure low levels of clonal cross contamination. Once the libraries pass the quality controls, the new resources will be made available in a format consistent with major applications. To improve the overall process efficiency, a set of Standard Operating Procedures (SOP's) will be developed to move BAC cloning away from an art form towards a routine high-throughput procedures. It is expected that the new BAC resources will contributed towards a routine-high throughput procedure. It is expected that the new BAC resources will contribute to better understanding of gene function and evolution through comparison of genes in a spectrum of animal species. The BAC clones will also become tools to create animal models for human diseases. Widespread dissemination of the clones is an unfunded but essential component of this proposal. Library information will be disseminated by publication and through our home page (http://www.chori.org/bacpac).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的是为许多高质量的物种准备BAC库，该物种是针对科学界对比较基因组学和功能应用的兴趣量身定制的。这项工作将由两个合作团队，一个由劳伦斯·伯克利国家实验室（LBNL）的成立博士领导的，另一个由Drs共同指导的奥克兰研究所的儿童医院奥克兰研究所。 Osoegawa＆Dejong。该应用具有四个与制备细菌性人工染色体（BAC）相关的组件：1）BAC库制备，2）克隆阵列和库重复，3）图书馆表征和4）研究与开发，以提高整体过程效率和BAC库质量。在第一年，该财团将产生和表征具有十倍基因组冗余的十二个动物BAC库。假定对额外的GBAC和提高的生产效率的需求越来越多，将分别在2年和3年内进行17和22个额外的图书馆。基于当前BAC克隆技术的库将补充并扩展BAC克隆收集的小曲目，现在可用于比较基因组分析。新的克隆收集将通过一组标准化的测试来分析，包括使用一组经济标记，有限的BAC末端测序，BAC指纹，BAC稳定性分析和插入尺寸确定。特别重要的是质量测试，以确保低BAC稳定性分析和插入尺寸确定。特别重要的是质量测试，以确保较低的克隆交叉污染。一旦图书馆通过质量控制，将以与主要应用程序一致的格式提供新资源。为了提高整体过程效率，将开发一组标准操作程序（SOP），以将BAC从艺术形式转移到常规的高通量程序。预计新的BAC资源将有助于常规的吞吐量程序。预计新的BAC资源将通过比较动物物种中的基因来更好地理解基因功能和进化。 BAC克隆也将成为创建人类疾病动物模型的工具。克隆的广泛传播是该提议的无资金供款但必不可少的组成部分。图书馆信息将通过出版物和我们的主页（http://www.chori.org/bacpac）传播。