MULTIPLEX IMMUNOASSAY FOR ANTIBODIES IN MACAQUES

猕猴抗体的多重免疫测定

• 批准号: 7715602
• 负责人: Paul Luciw
• 金额: $ 16.9万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715602
• 关键词: Acquired Immunodeficiency Syndrome; Animal Experimentation; Antibodies; Computer Retrieval of Information on Scientific Projects Database; Detection; Enzyme-Linked Immunosorbent Assay; Funding; Grant; Immunoassay; Infectious Agent; Institution; Macaca; Microspheres; Monkeys; Primates; Research; Research Personnel; Resources; Sampling; Serum; Source; System; Testing; Time; United States National Institutes of Health; Viral Antigens; Western Blotting; nonhuman primate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain healthy nonhuman primates for use in biomedical and AIDS related research, animals are routinely screened at most primate facilities for several infectious agents. In current practice, monkey serum samples are tested by conventional immunoassays such as enzyme linked immunosorbent assay (ELISA) or Western blot for the detection of specific antibodies. For testing antibodies against multiple agents in each sample, conventional immunoassays are laborious and time consuming. The objective is to develop more efficient immunoassays for serosurveillance of nonhuman primates. Accordingly, we have developed a novel multiplex serodiagnostic system where individually identifiable, fluorescent microbeads are coated with purified whole viral antigens.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：为了维护健康的非人类灵长类动物，用于生物医学和艾滋病相关研究，在大多数灵长类动物的设施中，对几种传染病的大多数设施进行了筛查。在目前的实践中，通过常规免疫测定法测试了猴子血清样品，例如连接的免疫吸附测定法（ELISA）或Western blot，以检测特定抗体。为了测试每个样品中针对多种药物的抗体，常规的免疫测定量很费力且耗时。目的是为非人类灵长类动物的血清监视开发更有效的免疫测定。 因此，我们开发了一种新型的多重血清诊断系统，在该系统中，可以单独识别的荧光微粒涂有纯化的全病毒抗原。