VIRAL LOCALIZATION BY IN SITU HYBRIDIZATION

通过原位杂交进行病毒定位

• 批准号: 7715420
• 负责人: Shawn Patrick O'Neil
• 金额: $ 1.3万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-05 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715420
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Anti-Retroviral Agents; Asians; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytomegalovirus; DNA Sequence; Funding; Grant; HIV; HIV Infections; Human; Human Herpesvirus 4; In Situ Hybridization; Infection; Infection prevention; Institution; Localized; Macaca; Measures; Modeling; Molecular Probes; Monkeys; Parvovirus; Pathogenesis; RNA; Research; Research Personnel; Resources; SIV; SRV-1; Saimiri; Simian virus 40; Source; System; Tissues; United States National Institutes of Health; Viral; Virus; design; pathogen; simian human immunodeficiency virus; vaccine efficacy; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infection of Asian macaque monkeys with simian immunodeficiency virus (SIV) and SIV/HIV chimeric viruses (SHIVs) serves as an important animal model for studying the pathogenesis of HIV infection and AIDS. In addition, this model provides a challenge system for evaluating the efficacy of vaccines and antiretroviral agents designed to prevent infection or AIDS in humans. We have designed several molecular probes for localizing viral RNA in tissues of monkeys infected with various isolates and clones of SIV and SHIV. These probes are used to measure the quantity and distribution of infected cells in the tissues of infected monkeys. We also have probes that hybridize with target RNA or DNA sequences of several other viral pathogens, including HIV, SRV-1, CMV, SV40, EBV, Herpes saimiri, and parvovirus.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 亚洲猕猴的感染是用猿猴免疫缺陷病毒（SIV）和SIV/HIV嵌合病毒（SHIVS）的感染，是研究HIV感染和AIDS发病机理的重要动物模型。 此外，该模型还提供了一种挑战系统，用于评估旨在防止感染或辅助人类的疫苗和抗逆转录病毒剂的功效。 我们设计了几种分子探针，用于将病毒RNA定位在感染了SIV和SHIV的各种分离株和克隆的猴子组织中。 这些探针用于测量感染猴子组织中感染细胞的数量和分布。 我们还进行了与其他几种病毒病原体的靶RNA或DNA序列杂交的探针，包括HIV，SRV-1，CMV，SV40，EBV，Saimiri和Parvovirus。