Using novel pre-harvest, resistance-conferring treatments to prevent foodborne pathogenic outbreaks in produce

使用新颖的收获前赋予抗性的处理方法来预防农产品中食源性致病菌的爆发

• 批准号: 10546010
• 负责人: Murli Manohar
• 金额: $ 30万
• 依托单位: ASCRIBE BIOSCIENCE INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-25 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10546010
• 关键词: Address; Agriculture; Alfalfa; Anions; Biological Sciences; Broccoli - dietary; Cantaloupes; Cessation of life; Chlorine; Consumption; Country; Development; Disease; Disease Outbreaks; Effectiveness; Enteral; Escherichia coli; European; Farm; Food; Food production; Formulation; Germination; Growth; Harvest; Health; Health Food; Hospitalization; Human; Hypochlorite; Industry Standard; Infection; Lead; Life; Link; Listeria; Listeria monocytogenes; Longevity; Measures; Microbe; Pathogenicity; Perception; Phase; Plant Roots; Plants; Positioning Attribute; Prevention; Process; Production; Public Health; Refrigeration; Resistance; Risk; Safety; Sales; Salmonella; Salmonella enterica; Seeds; Soil; Source; Surface; Technology; Testing; Time; Tissues; Tomatoes; Toxicology; Trigonella foenum-graecum; base; carcinogenicity; consumer behavior; defense response; design; efficacy testing; enteric pathogen; foodborne; foodborne illness; foodborne outbreak; foodborne pathogen; fruits and vegetables; improved; innovation; microbial; microbiome; microbiota; new technology; novel; pathogen; pathogenic bacteria; prevent; response; treatment effect

Abstract - Ascribe Bioscience is developing a novel technology to prevent foodborne outbreaks and recalls caused by the consumption of fresh or minimally processed fruits and vegetables. Product recalls and outbreaks of foodborne illness associated with fresh produce are on the rise in the U.S. In 2015, two outbreaks of E. coli O26 linked with fresh tomato salsa were responsible for 60 illnesses and 22 hospitalizations. In 2011, a multistate outbreak of Listeria linked to whole cantaloupes led to 143 hospitalizations and 33 deaths. Another cantaloupe-linked outbreak caused by Salmonella caused 261 cases across 24 states, with 94 hospitalizations and 3 deaths. Since most fresh produce is consumed raw, with no processing step to eliminate or minimize pathogens, they remain a major source of contamination. Fresh produce can become contaminated with pathogens at any steps of food production—during harvesting, or at various post-harvest stages including processing, transport, and storage. Continued increase of farm-to-fork distances further amplifies the risk of product contamination with pathogens, creating a significant—but largely preventable—public health burden. Chlorine-based treatments have commonly being used to disinfect fresh and minimally processed fruits and vegetables, but they have limited effectiveness due to inaccessibility to internal tissues where pathogens can flourish unaffected. To prevents outbreaks of foodborne associated with fresh produce, Ascribe is developing a treatment technology based on a natural molecule that activates plant defenses that persists over time, offering reliable, long-term protection against a broad range of bacterial pathogens. Ascribe’s proposed treatment technology has the potential to dramatically improve the safety of raw produce by addressing both external and internal bacterial contamination. In response to seed, foliar spray, or root-drench application, plants deploy an array of defense responses, which result in enhanced resistance against a broad spectrum of agriculturally important plant pathogens. In this project, Ascribe will establish the feasibility of the technology for the prevention of recalls and outbreaks related to human consumption of fresh or minimally processed fruits and vegetables. The specific aims of this Phase I project are 1) Develop formulations for produce designed for application by soil drench and spraying, and determine their effects on germination, growth, and quality; 2) Test the efficacy of these formulations against human enteric pathogens Salmonella enterica in tomato and Listeria monocytogenes in broccoli; 3) Test the effect of these treatments on the shelf life of tomato and broccoli. These aims will position Ascribe to further develop this technology in Phase II, in which Ascribe will refine the formulations, test efficacy against additional pathogens, expand to other types of fruits and vegetables, and perform toxicology studies to support regulatory approval. If successful, Ascribe’s proposed technology will dramatically increase the safety of consuming fresh produce. The technology also has the potential to increase the shelf-life of fresh, cut, or minimally processed fruits and vegetables, increasing their accessibility to consumers.

抽象的 - Ascribe Bioscience 正在开发一种新技术来预防食源性疫情和召回 由于食用新鲜或经过最低限度加工的水果和蔬菜而导致产品召回和疫情爆发。 在美国，与新鲜农产品相关的食源性疾病呈上升趋势。2015 年，爆发了两起大肠杆菌病。 2011 年，与新鲜番茄酱有关的大肠杆菌 O26 导致 60 例疾病和 22 例住院。 与整个哈密瓜相关的多州李斯特菌疫情导致 143 人住院治疗，另有 33 人死亡。 与哈密瓜相关的沙门氏菌疫情导致 24 个州出现 261 例病例，其中 94 人住院治疗 和 3 人死亡，因为大多数新鲜农产品都是生吃的，没有经过加工步骤来消除或减少。 病原体，它们仍然是新鲜农产品可能被污染的主要来源。 食品生产任何步骤中的病原体——收获期间或收获后的各个阶段，包括 加工、运输和储存的距离不断增加，进一步加大了风险。 产品受到病原体污染，造成重大但基本上可以预防的公共卫生负担。 基于氯的处理通常用于对新鲜和最低限度加工的水果和蔬菜进行消毒。 蔬菜，但它们的有效性有限，因为它们无法进入病原体可以接触的内部组织 为了防止与新鲜农产品相关的食源性疾病的爆发，Ascribe 正在开发一种 基于天然分子的处理技术，可激活植物防御，并随着时间的推移持续存在，提供 Ascribe 提出的治疗方法提供可靠、长期的保护，抵御多种细菌病原体。 技术有潜力通过解决外部问题来显着提高原料产品的安全性 和内部细菌污染，植物会因种子、叶面喷洒或根部浸水而部署。 一系列防御反应，从而增强对广泛农业害虫的抵抗力 在这个项目中，Ascribe 将确定预防技术的可行性。 与人类食用新鲜或最低限度加工的水果和蔬菜有关的召回和爆发。 该第一阶段项目的具体目标是 1) 开发适合土壤施用的产品配方 2）测试淋水和喷洒的效果，确定其对发芽、生长和品质的影响； 这些制剂针对人类肠道病原体番茄中的肠道沙门氏菌和单核细胞增生李斯特氏菌 西兰花；3）测试这些处理对番茄和西兰花保质期的影响。 Ascribe将在二期进一步开发这项技术，其中Ascribe将完善配方、测试功效 针对其他病原体，扩展到其他类型的水果和蔬菜，并进行毒理学研究 如果成功，Ascribe 提出的技术将大大提高安全性。 该技术还有可能延长新鲜农产品、切好的农产品的保质期。 最低限度加工的水果和蔬菜，增加消费者对它们的获取。