Characterizing the preterm infant skin microbiome and microbial shifts that precede neonatal late onset sepsis (LOS)

描述早产儿皮肤微生物组的特征以及新生儿迟发性败血症 (LOS) 之前的微生物变化

基本信息

批准号：
10664071
负责人：
Jennifer Jane Schoch
金额：
$ 16.16万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-01 至 2027-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10664071
关键词：
16S ribosomal RNA sequencing Address Affect Antibiotics Antisepsis Award Bacteria Bathing Bioinformatics Birth Candida Caring Catheters Cesarean section Childhood Clinical Clinical Trials Collection Control Groups Corynebacterium Cutaneous Data Dermatology Development Disease Early identification Environment Ethnic Origin Evolution Exhibits Failure Florida Future Genus staphylococcus Gestational Age Goals Hospitalization Hospitals Humidity Incidence Infant Intervention K-Series Research Career Programs Klebsiella Knowledge Laboratories Lactobacillus Lead Leadership Learning Life Medicine Mentors Metagenomics Methods Microbe Natural History Neonatal Outcome Outpatients Parents Pathogenicity Physicians Predisposition Premature Infant Prevention Prevention strategy Race Research Research Activity Research Design Resources Risk Role Sample Size Sampling Science Scientist Sepsis Serratia Shotguns Site Skin Streptococcus Swab Testing Therapeutic Training Training Activity Training Programs Translational Research Universities Very Low Birth Weight Infant Work career career development career networking cohort college design experience extreme prematurity feeding gut microbes indexing innovation insight large datasets late onset sepsis metagenomic sequencing microbial microbiome microbiome composition microbiome research microbiota transplantation mortality neonatal sepsis novel opportunistic pathogen pathogen pathogen exposure pathogenic bacteria perinatal complications preterm newborn programs recruit sex skills skin barrier skin microbiome skin microbiota skin organogenesis statistics surveillance strategy vaginal microbiota

项目摘要

PROJECT SUMMARY Despite recent emphasis on the microbiome, there are few skin microbiome studies in infants; even fewer have been performed in preterm infants. Currently, there is a significant gap in our knowledge of the normal evolution of the preterm infant skin microbiome from birth to an independent, site-specific cutaneous microbiome. Preliminary studies show that preterm infants have limited microbial diversity and different predominant cutaneous microbes compared to full-term infants. This lack of a robust, diverse skin microbiome may render preterm infants susceptible to pathogenic skin bacteria that cause disease, particularly neonatal sepsis. The research goals of this mentored career-development award are to: 1) characterize the establishment of the neonatal skin microbiome in a cohort of preterm infants from birth to 4 weeks and compare to demographic and clinical variables, 2) recruit a cohort of term infants to determine if the preterm infant skin microbiome is significantly different than term infants, and 3) analyze cultured pathogens with the skin microbiome in late-onset neonatal sepsis (LOS). Our methods represent a departure from the currently available studies, which provide opportunistic sampling of the neonatal skin microbiome rather than sampling at defined timepoints. This study will vertically-advance the field by sampling the preterm skin microbiome systematically after birth. The role of the skin microbiome in neonatal sepsis has also not been described, and the design of this study will provide a better understanding of changes in the skin microbiome before late onset sepsis. Together, these aims will provide insight into the normal and abnormal development of the preterm infant skin microbiome. Future studies will then leverage the neonatal skin microbiome via therapeutic and preventative strategies to lower the incidence of LOS. This K23 is the next logical step in the training program started under my KL2 award, and the final step in my transition to an independent physician scientist. This proposal describes mentored research and training activities designed to launch a novel neonatal skin microbiome research program, the first in the field of pediatric dermatology. Five training and career development goals, facilitated by my mentoring team, are required to accomplish this. These include: extend my understanding of research design, deepen my knowledge of microbiome and relevant laboratory science, gain working knowledge of statistics/bioinformatics (including advanced metagenomic analyses), learn to lead an independent laboratory, and expand my professional network within microbiome science. The University of Florida provides a rich environment with expertise in the neonatal microbiome and metagenomics, ideal to extend my previous work as I develop a novel neonatal skin microbiome research program. The resources at UF will be augmented by additional skin microbiome expertise and laboratory resources from Dr. Pammi at Baylor College of Medicine and Diversigen Inc.

项目概要尽管最近强调微生物组，但针对婴儿的皮肤微生物组研究却很少。更少有已在早产儿中进行过。目前，我们对正常进化的认识存在很大差距早产儿皮肤微生物组从出生到独立的、特定部位的皮肤微生物组。初步研究表明，早产儿的微生物多样性有限，且主要微生物群落不同。与足月婴儿相比，皮肤微生物。缺乏强大、多样化的皮肤微生物组可能会导致早产儿容易受到致病性皮肤细菌的感染，从而引起疾病，特别是新生儿败血症。这该指导性职业发展奖的研究目标是： 1) 描述建立一组早产儿从出生到 4 周的新生儿皮肤微生物组，并与人口统计学和临床变量，2) 招募一组足月婴儿以确定早产儿皮肤微生物组是否与足月婴儿显着不同，3) 分析晚发型婴儿皮肤微生物培养的病原体新生儿败血症（LOS）。我们的方法与当前可用的研究不同，这些研究提供了对新生儿皮肤微生物组进行机会性采样，而不是在规定的时间点采样。这项研究将通过在出生后系统地对早产儿皮肤微生物组进行采样，垂直推进该领域。的作用新生儿败血症中的皮肤微生物组也尚未被描述，本研究的设计将提供更好的了解迟发性脓毒症之前皮肤微生物组的变化。这些目标共同将提供深入了解早产儿皮肤微生物组的正常和异常发育。未来的研究将通过治疗和预防策略利用新生儿皮肤微生物组来降低 LOS 的发生率。这个 K23 是我在 KL2 奖项下开始的培训计划的下一个合乎逻辑的步骤，也是我的最后一步过渡为独立医师科学家。该提案描述了指导性研究和培训活动旨在启动一项新颖的新生儿皮肤微生物组研究项目，这是儿科领域的首个项目皮肤科。在我的指导团队的推动下，需要实现五个培训和职业发展目标完成这个。其中包括：扩展我对研究设计的理解，加深我对微生物组和相关实验室科学，获得统计学/生物信息学的工作知识（包括先进的宏基因组分析），学习领导独立实验室，并扩展我的专业网络在微生物组科学中。佛罗里达大学提供了一个拥有新生儿专业知识的丰富环境微生物组和宏基因组学，是我开发新型新生儿皮肤微生物组时扩展我之前工作的理想选择研究计划。佛罗里达大学的资源将通过额外的皮肤微生物组专业知识和贝勒医学院 Pammi 博士和 Diversigen Inc. 提供的实验室资源