MOLECULAR REGULATION OF GABAA RECEPTORS IN THE AMYGDALA

杏仁核中 GABAA 受体的分子调控

• 批准号: 7715786
• 负责人: KERRY J. RESSLER
• 金额: $ 2.14万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715786
• 关键词: Amygdaloid structure; Behavior; Computer Retrieval of Information on Scientific Projects Database; Extinction (Psychology); Funding; Genes; Grant; Hippocampus (Brain); Institution; Learning; Molecular; Regulation; Reporting; Research; Research Personnel; Resources; Site; Small Interfering RNA; Source; United States National Institutes of Health; Virus; Work; conditioned fear; gephyrin; mRNA Expression; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Analyses of GABA-A mRNA expression at baseline, following conditioned fear learning and following extinction of conditioned fear were completed during the reporting period. Additionally, we found significant effects on behavior when the GABA-alpha1 subunit is deleted from hippocampus and amygdala. Work also began on regional deletion of the GABA-gamma2 gene as well as site specific knockdown, using siRNA viruses, of the GAD67 and Gephyrin genes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在报告期内完成了基线、条件性恐惧学习和条件性恐惧消退后 GABA-A mRNA 表达的分析。 此外，我们发现当 GABA-α1 亚基从海马体和杏仁核中删除时，会对行为产生显着影响。 还开始了 GABA-gamma2 基因的区域删除以及使用 siRNA 病毒对 GAD67 和 Gephyrin 基因进行位点特异性敲除的工作。