MECHANISMS OF ORAL SIV TRANSMISSION

口腔 SIV 传播机制

• 批准号: 7715703
• 负责人: Ruth Margrit Ruprecht
• 金额: $ 8.16万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715703
• 关键词: Acetic Acid; Acetic Acids; Adolescent; Cheek structure; Computer Retrieval of Information on Scientific Projects Database; Data; Dose; Funding; Grant; Hamsters; Inflammation; Injection of therapeutic agent; Institution; Macaca mulatta; Modeling; Monkeys; Mucous Membrane; Oral; Oral cavity; Oral mucous membrane structure; Personal Satisfaction; Primates; Procedures; Research; Research Personnel; Resources; Source; Submucosa; Testing; Ulcer; United States National Institutes of Health; Virus; chemokine; transmission process

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to test whether inflammation of the oral mucosa in juveniles facilitates virus transmission after oral exposure, we established a primate model for transient oral inflammation. We used local injection of 10% acetic acid into the submucosa of the right cheek of anesthetized monkeys to induce inflammation in the mouth; this procedure has been well established in hamsters. We determined the dose needed in rhesus macaques to generate an inflammation with few mild ulcers. Our data showed a significant increase of oral SHIV-1157ipd3N4 transmission in the presence of oral inflammation in juvenile rhesus macaques compared to controls. We continue to examine the expression of chemokine coreceptors in oral mucosal tissues.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为了测试青少年口腔粘膜炎症是否会促进口腔暴露后病毒的传播，我们建立了灵长类动物短暂性口腔炎症模型。 我们对麻醉猴的右脸颊黏膜下层局部注射10%醋酸，诱发口腔炎症；这个程序已经在仓鼠身上得到了很好的证实。我们确定了恒河猴产生炎症且很少出现轻度溃疡所需的剂量。 我们的数据显示，与对照组相比，幼年恒河猴在存在口腔炎症的情况下，口腔 SHIV-1157ipd3N4 传播显着增加。 我们继续检查口腔粘膜组织中趋化因子辅助受体的表达。