HORNET Center for Autonomic Nerve Recording and Stimulation Systems (CARSS)

HORNET 自主神经记录和刺激系统中心 (CARSS)

• 批准号: 10557006
• 负责人: Maral Mousavi
• 金额: $ 76.71万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-23 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10557006
• 关键词: Acetylcholine; Adopted; Antibodies; Bladder; Calibration; Catecholamines; Chemicals; Colonic Pouches; Detection; Development; Disease; Electrodes; Elements; Encapsulated; Ensure; Enzymes; Equilibrium; Esophagus; Evaluation; Exocrine Glands; Film; Implant; In Vitro; Individual; Iridium; Label; Liquid substance; Longevity; Lung; Measures; Membrane; Methodology; Modality; Modification; Monitor; Movement; Myocardium; Nervous system structure; Neuromodulator; Neurons; Norepinephrine; Organ; Organ Temperatures; Parasympathetic Nervous System; Performance; Periodicity; Peripheral; Platinum; Polyurethanes; Polyvinyls; Relaxation; Risk; Role; Saline; Scanning; Sense Organs; Serum; Shapes; Smooth Muscle; Sphincter; Stomach; Stretching; Surface; Surgical sutures; System; Technology; Temperature; Temperature Sense; Testing; Thinness; Work; aptamer; autonomic nerve; base; cell motility; design; implantation; in vivo; iridium oxide; multiplex detection; neuromuscular; neuroregulation; parylene; parylene C; receptor; sensor

Physical and chemical sensing enables detection of functional states of individual end organs for state- dependent neuromodulation. NEST 5 focuses on developing robust sensors for temperature, motility, pH, acetylcholine, and catecholamines, and performing extensive benchtop testing to ensure reliable in vivo performance for end organ sensing. Proper functioning of end-organs is dependent on the local organ temperature. Therefore, accurate, in vivo temperature sensing of end organs can provide valuable information on their functional states. We will develop micropatterned platinum thin film-based resistive temperature sensors encapsulated in Parylene C for in vivo temperature sensing. Motility of certain end organs is associated with their functional states such as esophagus expansion and sphincter contraction. We will develop resistive and capacitive strain sensors encapsulated in Parylene C or polyurethane for motility sensing of target end organs. We will conformally coat commercial bend sensors with Parylene C as encapsulation and suture them in a pre-bent configuration to convert contraction and expansion to bending deformations. In parallel, we will conformally coat a commercial stretchable capacitive sensor with polyurethane as stretchable encapsulation. Acetylcholine functions both as a neuromuscular transmitter and neuromodulator, and is found in the central and peripheral neurons, in target organs of the parasympathetic nervous systems. NEST 5 develops electrochemical acetylcholine sensor using an enzyme-free sensing membrane doped with an organic and stable receptor. We will integrate the sensing membrane in a Parylene-C based platinum microarray. The end-organ electrochemical sensing of pH can be used for detecting functional states of individual end organs for enabling state-dependent neuromodulation. We will develop a platinum microarray on a Parylene substrate and electrodeposit iridium oxide as the active pH sensing material. The end-organ electrochemical sensing of catecholamines, and norepinephrine (NE) in particular, can be used for detecting the efficacy of autonomic neuromodulation on restoring the sympathetic-parasympathetic balance, as well as functional or disease states in organs and the body. We will develop a platinum microarray on a Parylene substrate and detecting catecholamines using the established methodology of fast scan cyclic voltammetry. Extensive thermally accelerated benchtop testing will be performed in saline for all sensing modules to de-risk the technology for long-term end organ sensing. Electrochemical sensing modules will be tested pooled serum media (in addition to saline solutions) for evaluation of sensor biofouling.

物理和化学传感能够检测各个末端器官的功能状态 依赖性神经调节。 NEST 5 专注于开发强大的温度、运动性、pH 值、 乙酰胆碱和儿茶酚胺，并进行广泛的台式测试以确保体内可靠 终末器官传感的性能。 终末器官的正常功能取决于局部器官的温度。因此，准确、体内 末端器官的温度传感可以提供有关其功能状态的有价值的信息。我们将开发 微图案铂薄膜电阻温度传感器封装在聚对二甲苯 C 中，用于体内 温度传感。 某些终末器官的运动与其功能状态有关，例如食道扩张和 括约肌收缩。我们将开发封装在聚对二甲苯 C 或聚对二甲苯中的电阻式和电容式应变传感器 用于目标末端器官运动传感的聚氨酯。我们将保形涂覆商用弯曲传感器 Parylene C 作为封装并将其缝合在预弯结构中以转换收缩和膨胀 到弯曲变形。与此同时，我们将在商用可拉伸电容传感器上保形涂覆 聚氨酯作为可拉伸封装。 乙酰胆碱既作为神经肌肉递质又作为神经调节剂，存在于中枢神经系统中。 以及副交感神经系统靶器官中的周围神经元。 NEST 5 开发 电化学乙酰胆碱传感器采用无酶传感膜，掺杂有机且稳定的 受体。我们将把传感膜集成到基于聚对二甲苯-C 的铂微阵列中。终末器官 pH 值的电化学传感可用于检测各个末端器官的功能状态，以实现 状态依赖性神经调节。我们将在聚对二甲苯基板上开发铂微阵列 电沉积氧化铱作为活性pH传感材料。 儿茶酚胺，特别是去甲肾上腺素 (NE) 的终末器官电化学传感可以 用于检测自主神经调节对恢复交感-副交感神经的功效 平衡以及器官和身体的功能或疾病状态。我们将开发铂金微阵列 在聚对二甲苯基底上并使用已建立的快速扫描循环方法检测儿茶酚胺 伏安法。所有传感将在盐水中进行广泛的热加速台式测试 模块来降低长期末端器官传感技术的风险。电化学传感模块将 测试了混合血清培养基（除了盐水溶液）以评估传感器生物污垢。