Cores

核心

基本信息

批准号：
7681640
负责人：
ALBERT B REYNOLDS
金额：
$ 21.53万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7681640
关键词：
3-Dimensional Address Adopted American Type Culture Collection Animal Experiments Appendix Architecture Arts Basement membrane Behavior Biological Assay Biological Models Biology Books Cancer Biology Cancer Center Caring Cell Line Cell model Cell-Cell Adhesion Cells Clutterings Collagen Color Computer software Computers Condition Confocal Microscopy Consultations Core Facility Cultured Cells D Cells Daily Data Data Collection Data Storage and Retrieval Depth Elements End Point Engineering Ensure Equipment Equipment and supply inventories Evaluation Event Exercise Exhibits Experimental Models Extracellular Matrix Fibronectins Fingerprint Freezing Funding Genes Goals Green Fluorescent Proteins Housing Human Resources Image Individual Internal Ribosome Entry Site Intranet Investments Knock-out Laboratories Laminin Lasers Learning Legal patent Licensing Ligation Light Link MCF10A cells Maintenance Malignant Neoplasms Mammalian Cell Maps Membrane Proteins Messenger RNA Methodology Methods Microscope Microscopy Modeling Modification Neomycin Numbers Oncogenes Operative Surgical Procedures Parents Participant Phase Policies Population Postdoctoral Fellow Procedures Protocols documentation Puromycin Quality Control Reagent Reproducibility Research Research Infrastructure Resources Retroviridae Role Running Sampling Savings Scanning Schedule Serum Services Shuttle Vectors Site Small Interfering RNA Sorting - Cell Movement Source Standards of Weights and Measures Structure System Technical Expertise Technology Testing Thick Three-dimensional analysis Time Tissues Training Transcript Tumor Suppressor Proteins Universities Variant Vial device Virus Work Wrestling Zeocin base cell behavior cost cost effective day design desire digital experience fetal fluorescence microscope in vivo Model instrument instrumentation interest mathematical model matrigel member membrane model monolayer mouse model new technology prevent programs reconstitution repository research study software systems three dimensional structure tumor tumor progression vector

项目摘要

General rationale and organization of the Cores The main potential technical barriers to efficient execution of the biology are (1) the quality control and execution of the 3-D culture system, and (2) the requirement for expertise and ready access to state of the art confocal microscopy. To make these essential technologies readily available to everybody, and to coordinate the efforts of the various groups, we propose two core facilities, one to coordinate the 3-D culture methodology (3-D Core), and another to manage the confocal microscope (Confocal Core). Although the 3- D Cell Culture and Microscope Cores will be described separately, they will be located in adjacent labs and are designed to work together to standardize data collection procedures and provide a seamless transition from the 3-D culture phase to the confocal analysis phase of each study.

核心的一般原理和组织有效执行生物学的主要潜在技术障碍是（1）质量控制和 3-D 培养系统的执行，以及 (2) 对专业知识和随时获取最新技术的要求共焦显微镜。让每个人都能轻松获得这些基本技术，并协调在各个团体的努力下，我们提出了两个核心设施，一是协调3D文化方法（3-D Core），另一种用于管理共焦显微镜（Confocal Core）。虽然 3- D 细胞培养和显微镜核心将分别描述，它们将位于相邻的实验室，并且旨在共同努力标准化数据收集程序并提供无缝过渡从每项研究的 3-D 培养阶段到共聚焦分析阶段。