ShEEP Request for iNSCOPIX nVue System

SheEEP 对 iNSCOPIX nVue 系统的请求

• 批准号: 10534510
• 负责人: Priyattam J. Shiromani
• 金额: --
• 依托单位: RALPH H JOHNSON VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10534510
• 关键词: Abnormal Cell; Address; Affect; Alcohol consumption; Algorithms; Animals; Area; Behavior; Biological; Biosensor; Blood flow; Brain; Brain Injuries; Brain imaging; Calcium; Cells; Collaborations; Computer software; Data; Data Analyses; Data Store; Devices; Disease; Equipment; Female; Fluorescence; Fright; Funding; Genetic Engineering; Goals; Image; Individual; Joints; Manufacturer Name; Maps; Measures; Medical center; Microscope; Monitor; Motion; Mus; Nerve Degeneration; Neurodegenerative Disorders; Neuroglia; Neurons; Neurosciences; Output; Pathologic; Pattern; Post-Traumatic Stress Disorders; Productivity; Request for Applications; Research; Research Personnel; Rodent; Sample Size; Services; Sheep; Signal Transduction; Special Equipment; Substance abuse problem; System; Technology; Time; Training; Translational Research; Traumatic Brain Injury; Vendor; Visualization; analog; brain cell; brain circuitry; cell type; data acquisition; denoising; digital; fluorophore; independent component analysis; indexing; instrumentation; interest; lens; male; miniaturize; neurotransmitter release; peer; response; sex; solid state; student training; substance use; synergism; traumatic stress; user-friendly

The brain is no longer considered a “black box” because new devices and genetically- engineered biosensors have made it possible to monitor activity of cells deep in the brain. However, the brain is comprised of many different types of cells, and a device is needed that monitors activity of different types of cells adjacent to each other especially in animals actively engaged in behavior. Over the last year a new breakthrough technology was made available - a miniature microscope that weighs only 2g, makes it possible to monitor activity of two different cells in freely-behaving rodents. This device identifies activity patterns of brain cells during specific behaviors, allowing investigators to construct brain activity maps of specific brain circuits regulating purposeful behavior. Researchers can determine how neurodegenerative disease or specific conditions such as traumatic brain injury or substance abuse affects circuit function. The nVue miniscope system enables investigators to identify activity at the single cell level and how dysfunctional activity at the local level can cause pathological behavior. However, the necessary core instrumentation that would serve to measure critical indices of cell signaling, quantification and imaging, are not currently available to an individual investigator at RHJ VAMC. The instrumentation requested in this proposal will directly address this issue. The underlying hypothesis of this proposal is that abnormal cell signaling in specific brain circuits leads to pathological behavior. A device is needed to monitor activity of brain circuits. This equipment will accomplish the following specific aims: i) image brain circuits activated during purposeful behavior, and ii) identify change in circuit activity in response to disease, substance abuse, and traumatic stress or brain injury. By mapping activity in local circuits it will be possible to reestablish activity and restore normal behavior.

大脑不再被认为是一个“黑匣子”，因为新的设备和基因- 工程生物传感器使得监测大脑深处细胞的活动成为可能。 然而，大脑由许多不同类型的细胞组成，需要一种设备来 积极监测彼此相邻的不同类型细胞的活动，特别是在动物中 去年，一项新的突破性技术出现了—— 重量仅 2 克的微型显微镜，可以监测两种不同的活性 该装置可识别自由行为的啮齿动物的脑细胞活动模式。 特定行为，允许研究人员构建特定大脑的大脑活动图 研究人员可以确定神经退行性病变的机制。 疾病或特定情况（例如脑外伤或药物滥用）会影响回路 功能。 nVue 微型显微镜系统使研究人员能够识别单细胞的活动 水平以及局部水平的功能失调活动如何导致病理行为。 用于测量细胞信号传导关键指标的必要核心仪器， 定量和成像，目前 RHJ 的个体研究人员无法使用 本提案中要求的仪器将直接解决这个问题。 该提议的基本假设是特定细胞信号传导异常 大脑回路会导致病理行为，需要一种设备来监测大脑的活动。 该设备将实现以下特定目标： i) 图像脑电路。 在有目的的行为期间被激活，并且 ii) 识别电路活动的变化以响应 通过绘制局部活动图来了解疾病、药物滥用和创伤性应激或脑损伤。 电路将有可能重新建立活动并恢复正常行为。