Functional Proton Electron Double Resonance Imaging

功能质子电子双共振成像

• 批准号: 7642583
• 负责人: Valery V Khramtsov
• 金额: $ 22.5万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7642583
• 关键词: Activities of Daily Living; Biological; Collaborations; Data; Development; Electrons; Female; Frequencies; Functional Imaging; Future; Goals; Image; Imaging Techniques; Label; Life; Magnetic Resonance Imaging; Mammary Neoplasms; Maps; Measurement; Medical; Medical Imaging; Medicine; Modality; Modification; Mus; Oxygen; Physiologic pulse; Power Sources; Protons; Radio; Ramp; Relaxation; Research Personnel; Resolution; Screening procedure; Side; Signal Transduction; Site; Slice; Specificity; Structure; Sulfhydryl Compounds; System; Techniques; Testing; Time; Tissues; Variant; base; bioimaging; clinical application; design; improved; in vivo; innovation; instrument; irradiation; magnetic field; nitroxyl; outcome forecast; public health relevance; research study; spectroscopic imaging; success; tumor; tumor progression

DESCRIPTION (provided by applicant): The overall goal of this project is to develop new modalities of functional imaging in living tissues. To reach this goal we will use a new concept of Variable Field (VF) and Variable Radio Frequency (VRF) proton electron double resonance imaging (PEDRI) in combination with design of specific paramagnetic probes. This will allow for functional mapping using (e.g. oxygen or pH mapping) within MRI high quality spatial resolution and short acquisition time. The specific aims are: (SA1) To develop Variable Field PEDRI for functional imaging of paramagnetic probes. The approach will allow for pH mapping using available probes, screening newly synthesized probes, and optimization of VRF PEDRI spectroscopic parameters for functional pH and oxygen mapping. (SA2) To develop PEDRI-oriented functional probes: nitroxyl and trityl radicals. Nitroxide probes with enhanced stability towards reduction, and dual function pH- and oxygen triarylmethyl probes will be synthesized and used for VF/VRF PEDRI. Synthesis an optimization of the probes is absolutely crucial for the overall efficiency of functional PEDRI applications. (SA3) To develop Variable Radio Frequency PEDRI for functional mapping of living tissues. VRF PEDRI is an instrumentally innovative modification of PEDRI approach. Compared with VF PEDRI this approach will use stationary magnetic field but slightly different EPR RF excitation frequencies resulting improved magnetic field homogeneity and stability, and decreased acquisition time. It will also provide opportunity for future development of a simplified PEDRI system based on conventional NMR gradients. The capacity of the functional PEDRI for in vivo pH and oxygen mapping will be demonstrated for the most effective probes in PyMT mammary tumors of FVB/N female mice. In a tumor microenvironment, both oxygen and pH changes are indicative of tumor progression being important factors for prognosis and treatment. In summary, the success of this project may have a significant impact on the future of in vivo functional imaging applications to medicine. PUBLIC HEALTH RELEVANCE: This project will develop new modalities of functional mapping of living tissues based on MRI of specific paramagnetic probes. Application experiments will use newly designed probes for pH- and oxygen mapping of mammary tumors in living mice. The developed technique may have a significant impact on the future applications to medical imaging.

描述（由申请人提供）：该项目的总体目标是开发活组织中功能成像的新方式。为了实现此目标，我们将使用新的可变场（VF）和可变射频（VRF）质子电子双共振成像（PEDRI）的新概念，并结合设计特定的顺磁性探针的设计。这将允许在MRI高质量空间分辨率和短时间获得时间内使用（例如氧或pH映射）功能映射。具体目的是：（SA1）开发用于顺磁性探针功能成像的可变场PEDRI。该方法将允许使用可用探针，筛选新合成的探针以及对功能性pH和氧映射的VRF PEDRI光谱参数的优化。 （SA2）开发面向PEDRI的功能探针：硝基氧基和Trityl自由基。氮氧化物探针具有增强稳定性的稳定性，并且将合成双重功能pH-和氧三氧化氢探针，并用于VF/VRF PEDRI。合成探针的优化对于功能性PEDRI应用的总体效率至关重要。 （SA3）开发可变的射频PEDRI来进行活组织的功能映射。 VRF Pedri是PEDRI方法的工具创新的修改。与VF Pedri相比，该方法将使用固定磁场，但EPR RF激发频率略有不同，从而改善了磁场的同质性和稳定性，并减少了采集时间。它还将为基于常规NMR梯度的简化PEDRI系统的未来开发提供机会。对于FVB/N雌性小鼠PYMT乳腺肿瘤中最有效的探针，将证明功能性PEDRI用于体内pH和氧图的能力。在肿瘤微环境中，氧气和pH变化都表明肿瘤进展是预后和治疗的重要因素。总而言之，该项目的成功可能会对医学中体内功能成像应用的未来产生重大影响。公共卫生相关性：该项目将基于特定顺磁性探针的MRI开发生物组织功能映射的新方式。应用实验将使用新设计的探针用于活小鼠乳腺肿瘤的pH和氧图。开发的技术可能会对未来对医学成像的应用产生重大影响。