Vitamin D and Healthy Aging: Establishing the Sled Dog Sentinel for the Circumpolar North

维生素 D 与健康老龄化：为北极圈建立雪橇犬哨兵

基本信息

批准号：
10649356
负责人：
Kriya L Dunlap
金额：
$ 7.75万
依托单位：
UNIVERSITY OF ALASKA FAIRBANKS
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10649356
关键词：
Adoption Affect Age Aging Alaska Alaska Native Alaskan Animal Model Arctic Regions Biochemical Pathway Biological Markers Biomedical Research Brain Canis familiaris Child Chronology Coin Consensus Consumption Custom Data Dementia Diabetes Mellitus Diet Dietary Intervention Dietary Supplementation Dietary intake Disease Diurnal Rhythm Eating Economics Elderly Exercise Exhibits Fishes Food GLUT 4 protein Glucose Transporter Goals Health Hormones Human Impaired cognition Impairment Indigenous Insulin Insulin Resistance Intake Intervention Studies Inuits Knowledge Laboratory Animals Life Style Ligands Link Longevity Magnetic Resonance Imaging Malignant Neoplasms Measures Metabolic Metabolic Diseases Metabolic Marker Metabolic syndrome Modeling Morphology Neurodegenerative Disorders North America Nutrient Nutritional Outcome Oxidative Stress Peripheral Blood Mononuclear Cell Persons Play Polyunsaturated Fatty Acids Population Prevalence Process Production Public Health Recommendation Reporting Research Rickets Risk Risk Factors Role Rural Salmon Sample Size Seasonal Variations Sentinel Serum Skin Sledding Societies Source Sun Exposure Sunlight Tissues UV Radiation Exposure Vitamin D Vitamin D Deficiency Vitamin D3 Receptor Yup&apos ik age effect aging brain brain health canine model experience gray matter gut health healthy aging high risk immune function improved inflammatory marker insulin signaling metabolic rate prevent prohormone response transcription factor trend western diet white matter

项目摘要

PROJECT SUMMARY Our long-term goal is to establish levels of vitamin D intake and vitamin D sufficiency for humans and canines living in the circumpolar north. Our overall objectives are to (i) define how chronological age impacts vitamin D status and morphological changes in the sled dog model, and (ii) determine the effects of salmon on vitamin D status and markers of metabolic syndrome and insulin signaling in old and young sled dogs. With their shorter lifespan and parallel biochemical pathways, dogs have become an important biomedical research model for both dementia related impairments and insulin signaling. Sled dogs, in particular, are an important human health model in the circumpolar north because they are part of the traditional subsistence lifestyle. Over the past 100 years, societies in North America and elsewhere in the world have transitioned towards the so-called “Western diet,” composed primarily of highly processed “factory foods”. This nutritional transition has been identified as playing a central role in current public health issues in peoples of the far north. Historically, Alaska Natives (AN) were able to consume adequate vitamin D from traditional foods, most notably from salmon, despite living at latitudes that prevent the synthesis of vitamin D in the skin from sunlight for much of the year. This is also true for village sled dogs. With the shift away from traditional foods, peoples of the circumpolar north are experiencing increased prevalence of vitamin D deficiency. Yup’ik children are developing rickets at higher rates while elders consistently exhibit higher serum vitamin D levels; this is a stark contrast to national trends. It is estimated that over 1 billion people worldwide are deficient in vitamin D. Vitamin D deficiency has been implicated in a multitude of diseases, including metabolic disorders, aging, and cognitive impairments. Still, little consensus has been reached on recommended intake to achieve adequate levels in humans and even less so in dogs. Therefore, there is a critical need to develop better understanding of vitamin D intake in the circumpolar north, where there is the highest risk of deficiency, to understand the links between diet, age, vitamin D status, and metabolic health. This study will begin to fill the gaps in knowledge pertaining to vitamin D deficiency with the following specific aims: 1) Correlate markers of brain aging with vitamin D status in an animal model of the circumpolar north and 2) Determine the effects of increased vitamin D from wild Alaska salmon on biomarkers of insulin signaling and metabolic health. Volume and morphology changes in brain gray and white matter will be assessed with magnetic resonance imaging in old and young dogs and correlated with serum vitamin D status. In addition to other markers of metabolic syndrome, the insulin responsive glucose transporter (GLUT4) on peripheral blood mononuclear cells will be measured in response to insulin in sled dogs of varying ages before and after a dietary supplementation with wild Alaskan Salmon. The expected outcomes of this project will lend to the insufficient data pertaining to vitamin D requirements, status, and health outcomes in populations that rely solely on dietary intake to obtain sufficiency.

项目概要我们的长期目标是确定人类和犬类的维生素 D 摄入量和维生素 D 充足量水平我们的总体目标是 (i) 确定实际年龄对维生素 D 的影响。雪橇犬模型的状态和形态变化，以及 (ii) 确定鲑鱼对维生素 D 的影响年老和年轻雪橇犬代谢综合征和胰岛素信号的状态和标志物。狗的寿命和平行的生化途径，已成为重要的生物医学研究模型尤其是与痴呆相关的损伤和胰岛素信号传导，对人类来说都是重要的。环极地北部的健康模式，因为它们是传统生存生活方式的一部分。过去 100 年来，北美和世界其他地方的社会已经转向所谓的“ 主要由高度加工的“工厂食品”组成的“西方饮食”已经发生了这种营养转变。历史上，阿拉斯加被认为在当前远北地区人民的公共卫生问题中发挥着核心作用。当地人 (AN) 能够从传统食物中摄入足够的维生素 D，尤其是鲑鱼、尽管生活在高纬度地区，但一年中的大部分时间，皮肤都无法合成维生素 D。对于乡村雪橇犬来说也是如此，随着环极地人民逐渐远离传统食物。北方地区的尤皮克儿童维生素 D 缺乏症患病率不断上升。较高的比率，而老年人的血清维生素 D 水平始终较高；这与全国形成鲜明对比；据估计，全球有超过 10 亿人缺乏维生素 D。维生素 D 缺乏症已成为人们关注的焦点。与多种疾病有关，包括代谢紊乱、衰老和认知障碍。尽管如此，对于人类和动物达到足够水平的推荐摄入量尚未达成共识。因此，迫切需要更好地了解狗的维生素 D 摄入量。环极地北部地区缺乏维生素D的风险最高，了解饮食、年龄、维生素 D 状况和代谢健康这项研究将填补维生素相关知识的空白。 D 缺乏症具有以下具体目标：1) 将大脑衰老标志物与维生素 D 状态相关联环极地北部的动物模型，以及 2) 确定来自野生动物的维生素 D 含量增加的影响阿拉斯加鲑鱼的胰岛素信号和代谢健康的生物标志物。将通过磁共振成像评估老年人和年轻人大脑灰质和白质的变化除了代谢综合征的其他标志物之外，还与狗的血清维生素 D 状态相关。外周血单核细胞上的胰岛素反应性葡萄糖转运蛋白 (GLUT4) 将在不同年龄的雪橇犬在膳食补充野生阿拉斯加之前和之后对胰岛素的反应鲑鱼。该项目的预期成果将归因于有关维生素 D 的数据不足。仅依靠膳食摄入来获得充足的人群的需求、状况和健康结果。