Core A: Animal Models Core

核心 A：动物模型核心

• 批准号: 10642788
• 负责人: Ryan B Sartor
• 金额: $ 24.73万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-19 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10642788
• 关键词: Animal Model; Animals; Bacteria; Breeding; Cell Communication; Cell Lineage; Cells; Chronic; Complex; Country; Creativeness; Crossbreeding; Cytoprotection; Derivation procedure; Development; Ecology; Environment; Epithelial Cells; Epithelium; Faculty; Funding; Genes; Genetic; Genetic Transcription; Germ-Free; Gnotobiotic; Goals; Homeostasis; Housing; Human; Human Resources; Immune; Immune response; Immunocompromised Host; Individual; Intestinal Mucosa; Intestines; Laboratories; Leadership; Maintenance; Mammals; Measures; Mediating; Microbe; Modeling; Mouse Strains; Mucous Membrane; Mus; Mutant Strains Mice; Phenotype; Physiological; Predisposition; Productivity; Proliferating; Proteins; Relapse; Reporter; Reporter Genes; Research Personnel; Resolution; Resources; Rodent; Role; Services; Stretching; Tissues; Transgenic Mice; Transgenic Organisms; Universities; Variant; Washington; Work; Zebrafish; animal facility; animal resource; cell type; cost; cost effective; experience; experimental study; fecal transplantation; germ free condition; gut inflammation; gut microbiota; improved; in vivo imaging; innovation; insertion/deletion mutation; intestinal barrier; intestinal homeostasis; microbial; microbiota; mouse model; mutant; novel; prevent; programs; response; success; wasting

The Animal Models Core centralizes breeding, development and maintenance of extremely useful, but complex mouse and zebrafish models by providing a stable environment for each centrally housed line to increase efficiency, decrease costs and prevent phenotype variation. This Core leverages exceptional specific pathogen free (SPF) and gnotobiotic mice and zebrafish facilities and expert personnel to provide high-quality resources and services to all 3 current and proposed Projects. Core A personnel work closely with Project investigators to provide SPF and gnotobiotic mouse and zebrafish models that are essential to accomplish goals of each of the current projects. This core provides prioritized creation of novel SPF mouse strains and derivation of germ-free (GF) lines that will support the success of Projects 1, 2, 3 and 4. In addition, this Core has created highly innovative zebrafish models to facilitate activities in Projects 3 and 4. By providing technical experience and central housing to create and maintain GF wild type, mutant and double mutant mouse strains, Core A allows investigators to efficiently perform innovative, mechanistic experiments far beyond the resources of traditional R01-supported investigators and extends the capabilities of our national gnotobiotic resource. These GF mice and zebrafish allows Project investigators to manipulate the complex gut microbial ecology to study the mechanistic role of luminal microbes on immune and epithelial responses in gnotobiotic animals colonized with defined microbiota. The Specific Aims of the Animal Models Core are to provide P01 Projects with: 1.Centralized breeding of specific pathogen free and germ-free mice and zebrafish to eliminate redundancy in breeding units, diminish expenses, decrease environmental variation by maintaining central housing facilities and minimize waste by promoting shared utilization of animals by multiple investigators; 2.Create new mouse and zebrafish models including importing existing mutant strains for central breeding, creating new strains, including cell-specific mutants by crossbreeding of existing mutant, transgenic and fluorescent protein reporter lines, and generating new transgenic and mutant lines; 3.Germ-free derivation of existing and new mouse strains that can be used to evaluate the impact of complex resident microbiota individual microbial species or microbial consortia on intestinal inflammation and mucosal homeostasis. This Core supports Projects 1, 2, 3 and 4 in the renewal application.

动物模型核心集中育种、开发和维护极其有用但 通过为每个集中饲养的线提供稳定的环境来构建复杂的小鼠和斑马鱼模型 提高效率、降低成本并防止表型变异。该核心利用了特殊的特殊 无病原体（SPF）和无菌小鼠和斑马鱼设施和专家人员，提供高质量 为所有 3 个当前和拟议项目提供资源和服务。 核心 A 人员与项目研究人员密切合作，提供 SPF 和限生小鼠以及 斑马鱼模型对于实现当前每个项目的目标至关重要。该核心提供 优先创建新型 SPF 小鼠品系并衍生无菌 (GF) 品系，以支持 项目 1、2、3 和 4 的成功。此外，该核心还创建了高度创新的斑马鱼模型 促进项目 3 和 4 中的活动。通过提供技术经验和中央住房来创建和 维持 GF 野生型、突变型和双突变型小鼠品系，Core A 使研究人员能够有效地 进行创新的机械实验，远远超出传统 R01 支持的资源范围 研究人员并扩展了我们国家知生资源的能力。这些 GF 小鼠和斑马鱼 允许项目研究人员操纵复杂的肠道微生物生态来研究其机制作用 腔内微生物对定植有特定微生物群的限生动物的免疫和上皮反应的影响。 动物模型核心的具体目标是为 P01 项目提供： 1、集中繁育无特定病原体、无菌小鼠、斑马鱼，消除 繁育单位的冗余，减少开支，通过维持中央环境来减少环境变化 住房设施并通过促进多个研究人员共享动物来最大限度地减少浪费； 2.创建新的小鼠和斑马鱼模型，包括导入现有的突变品系进行集中育种， 通过现有突变体、转基因和 荧光蛋白报告系，并产生新的转基因和突变系； 3.现有和新小鼠品系的无菌衍生，可用于评估影响 复杂的常驻微生物群单个微生物物种或微生物群落对肠道炎症和 粘膜稳态。 该核心支持续订申请中的项目 1、2、3 和 4。