HSP72 in Mitochondrial & Microtubular Protection in NEC

线粒体中的 HSP72

• 批准号: 7599657
• 负责人: JENNIFER L LIEDEL
• 金额: $ 12.46万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7599657
• 关键词: Animals; Apoptosis; Area; Basic Science; Binding; Binding Sites; Biology; Chicago; Childhood; Clinical; Complex; Confocal Microscopy; Critical Care; Data; Enterocytes; Epithelial; Epithelial Cells; Fellowship; Foundations; Future; HSP72 protein; Heat shock proteins; In Vitro; Inflammation; Intestines; Investigation; Knockout Mice; Laboratories; Medicine; Mentors; Microtubules; Mitochondria; Mitochondrial Membrane Protein; Modeling; Molecular and Cellular Biology; Mucositis; Neonatology; Prevention; Protein Binding; Relative (related person); Research; Research Personnel; Resistance; Role; Scientist; Site; Solid; Stress; Techniques; Testing; Time; Training; Transgenic Organisms; Universities; base; career; depolymerization; experience; in vivo; intestinal epithelium; monolayer; polymerization; prevent; skills; thermal stress

DESCRIPTION (provided by applicant): In this mentored clinical scientist training application, the applicant will test the hypothesis that heat shock protein (Hsp) 72 binds to both mitochondria and microtubules stabilizing them; thus making this complex resistant to exogenous insult and thereby preventing apoptosis in the intestinal epithelium and uncovering future avenues for the prevention of necrotizing eneterocolitis (NEC). The preliminary data strongly support this hypothesis. The studies outlined in this application are aimed at examining the specific mechanism(s) of the relationship between protection and the complex interaction between the mitochondria and microtubular network. These investigations will provide an excellent training opportunity in the areas of epithelial biology, confocal microscopy and the acquisition of techniques needed to apply the cellular observations to the whole animal. The applicant, Jennifer L. Liedel, MD, will complete fellowships in neonatology and pediatric critical care at the University of Chicago June 30, 2004. In 2000, during the first year of her four-year training period, she formally entered the laboratory of Dr. Eugene Chang to seek additional basic science training and to study the effects of heat shock proteins in protection of the intestinal epithelium and the prevention of apoptosis. The proposed training plan provides 75% protected time, exceptional opportunities to acquire new skills and experience in the areas of cellular and molecular biology as well as didactic training in techniques and proper research conduct. This plan also provides meaningful interactions with experienced investigators, allowing the applicant to mature into a successful independent investigator. The applicant's research will form a solid foundation upon which the future direction and research necessary to build a career in academic medicine can be based.

描述（由申请人提供）：在这项指导的临床科学家培训应用中，申请人将检验以下假设：热休克蛋白（HSP）72与线粒体和微管结合稳定它们。从而使这种复杂的抗外源性损伤具有抗性，从而防止了肠上皮的凋亡，并发现了预防坏死性结肠炎（NEC）的未来途径。初步数据强烈支持这一假设。本应用中概述的研究旨在检查线粒体和微管网络之间的保护与复杂相互作用之间关系的特定机制。这些研究将在上皮生物学，共聚焦显微镜以及对将细胞观测应用于整个动物应用所需的技术方面提供极好的培训机会。 申请人詹妮弗·L·利德尔（Jennifer L. Liedel），医学博士将在芝加哥大学（University of Chicago of Chicago of of Chicago of Chicago of of of Hereen Chance of Eptium of Apith of Protistion of Protistion of Apith of Protistion of Protistium of Protistium of Protistium of Protistium of Protistium of Protistium和InteStrestical opith of Protive of apith of apith and，申请人将在芝加哥大学完成新生儿学和儿科重症监护奖学金。拟议的培训计划提供了75％的受保护时间，在细胞和分子生物学领域获得新技能和经验的特殊机会以及技术和适当的研究行为的教学培训。该计划还提供了与经验丰富的调查人员的有意义的互动，使申请人能够成熟成为成功的独立研究者。申请人的研究将构成一个坚实的基础，在该基础上，可以基于建立学术医学职业所必需的未来方向和研究。