CORE--ANIMAL

核心--动物

• 批准号: 7614192
• 负责人: DRAKE MORGAN
• 金额: $ 17.38万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7614192
• 关键词: Animal Model; Animals; Autoradiography; Behavioral; Brain; Chemistry; Cocaine; Condition; Connexins; Coupled; Exposure to; Goals; Grant; Heroin; Image; Investigation; Length; Ligands; Longitudinal Studies; Molecular Biology; Monkeys; Motor Activity; Neurobiology; Procedures; Rattus; Recording of previous events; Research Personnel; Schedule; Self Administration; Self-Administered; Testing; Time; Tissue Banks; Tissues; Training; brain tissue; deprivation; drug of abuse; monoamine; neuroadaptation; nonhuman primate; novel; programs

The overall objectives of the Animal Core are to provide animals to the various projects in the Center, and to organize and store tissue from non-human primates as needed by the projects in the Center. Many of the projects described in this grant will use animal models of self-administration to study long-term neuroadaptations as a consequence of exposure to drugs of abuse. Rats will be trained to self-administer cocaine, heroin, or cocaine and heroin (speedball) for varying lengths of time under different schedule conditions coupled with various periods of deprivation, in addition, rats treated with novel irreversible ligands at the monoamine transporters will be studied behaviorally in locomotor activity studies and prepared for autoradiographic analysis. Finally, the Animal Core will be responsible for proper sacrifice, brain collection, tissue storage, and redistribution of brains from non-human primates used in various projects, when necessary. The specific goals of the Animal Core are as follows: 1. Provide experimental animals to subprojects 0001, 0008, 0011, and 0012 that have particular histories of self-administration and deprivation. 2. Examine the locomotor-altering effects of novel compounds (administered i.c.v.) produced by the Chemistry Core. 3. Provide subjects that have been treated with these novel compounds (administered i.c.v.) to subproject 0001 for autoradiographic analysis. 4. Collect and store brain tissue from non-human primates (from subprojects 0012, 0003, and 0009) when the need arises, and redistribute the tissue to subprojects for neurobiological investigation.

动物核心的总体目标是为中心的各个项目提供动物，并根据中心的项目需要组织和存储来自非人类灵长类动物的组织。这笔赠款中描述的许多项目将使用自我管理的动物模型来研究长期神经适应的因素，这是由于暴露于滥用药物而导致的。在不同时间表条件下，在不同时间表的条件下，在不同的时间表的情况下，将对大鼠进行自动加工可卡因，海洛因或可卡因和海洛因（速球）的培训，以在不同的不可逆配体进行单胺转运蛋白的新型不可逆的配体进行自动分析和准备的自动研究。最后，动物核心将负责适当的牺牲，大脑收集，组织储存以及在必要时在各种项目中使用的非人类灵长类动物的大脑重新分布。 动物核心的具体目标如下： 1。向具有特定自我给药和剥夺历史的0001、0008、0011和0012提供实验动物。 2。检查新型化合物的运动改变作用（施用的I.C.V.） 由化学核心产生。 3。提供已经用这些新型化合物处理的受试者（施用 I.C.V.）至子标记0001进行放射自显影分析。 4。从非人类灵长类动物（来自0012、0003和0009）收集和存储脑组织，并在需要时，并将组织重新分配至神经生物学 调查。