Small Animal Imaging Resource

小动物成像资源

• 批准号: 7490290
• 负责人: David R. Vera
• 金额: $ 13.42万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7490290
• 关键词: Air; Analytical Chemistry; Anesthesia procedures; Animal Model; Animals; Autoradiography; Biochemical; Biometry; Blood; Blood Vessels; Cancer Center; Caring; Chemistry; Cities; Clinical Research; Communities; Comprehensive Cancer Center; Computer software; Cyclotrons; Data; Data Set; Development; Dextrans; Diagnostic; Disease regression; Electrocardiogram; Equilibrium; Freezing; Gadolinium DTPA; Gadopentetate Dimeglumine; Goals; Greater sac of peritoneum; Heart Rate; Heating; Histology; Hour; IACUC; Image; Imaging Device; Imaging Techniques; In Situ; Informatics; Institutes; Intervention; Intestines; Isoflurane; Kinetics; Left; Life; Localized; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Measurement; Metric; Modality; Modeling; Monitor; Motion; Mus; New Agents; Optics; Organ; Perfusion; Physiologic Monitoring; Physiologic pulse; Physiological; Pliability; Positioning Attribute; Positron-Emission Tomography; Protocols documentation; Pulse taking; RF coil; Radiochemistry; Radiology Specialty; Recovery; Relaxation; Research Personnel; Resolution; Resources; Respiration; Rodent; Saline; Sampling; Scheme; Services; Site; Solid Neoplasm; Solutions; Spectrum Analysis; Spin Labels; Sterility; System; Temperature; Time; Tissues; Ultrasonography; Ventilator; Ventricular; anticancer research; chemical kinetics; cost; dextran; digital; experience; in vivo; in vivo Cellular and Molecular Imaging Centers; member; molecular imaging; optical imaging; receptor density; size; tool; tumor; tumor growth

The Department of Radiology at UCSD has implemented a Small Animal Imaging Resource (SAIR) to support cancer research at UCSD and the San Diego bioresearch community. The SAIR is located in the vivarium at the Rebecca and John Moores UCSD Comprehensive Cancer Center (CC) on the La Jolla campus. This facility supports the efforts of the ICMIC investigators and the UCSD cancer research investigators and then Campus and city-wide efforts. Some non-UCSD potential users include: Sidney Kimmel CC, Scripps, Burnham and Salk Institutes, and the San Diego biotech companies. The UCSD SAIR will provide MRI, optical, CT, ultrasound, High-Resolution planar gamma imaging, and PET of rodents, as well as high-resolution digital autoradiography and fluorescent imaging of thin whole body rodent sections. Except for MRI, all modalities are at one site adjacent to the CC vivarium. Support and expertise includes optical imaging, animal support, image computation, MR & optical hardware and software, diagnostic agent chemistry, radiochemistry including a cyclotron, analytical chemistry, kinetic modeling, informatics, histology, and a vascularized solid tumor model to cost-effectively screen imaging paradigms. Our primary emphasis will be the acquisition of images for kinetic modeling. For this reason the kinetic modeling service will develop automated data reduction schemes. Our previous experience with the use of kinetic modeling for receptor density measurement from clinical studies has convinced us that the data reduction path from the image data set must be completely automated. There are two reasons for this: first is high throughput, and second and more important is the absence of operator intervention and the elimination of operator bias. This latter quality is essential for rigorous statistical analysis (by the ICMIC Biostatistics Service) of the imaging and biochemical/physiologic metrics. High-resolution ultrasound imaging will permit quantification of tumor size as well as measurement of left ventricular volume for recovery correction of PET sampled input functions. Most importantly, it will permit the use of in situ tumor models. A key contribution of ultrasound to cancer research in mice is the ability to image in sterile fashion and to do so without the need for anesthesia. This should permit frequent monitoring of tumor growth or regression following interventions. Members of the ICMIC Imaging Core will interact closely with colleagues of the Animal Models & Care and the IMCIC Biostatistics Service. Our goal will be the identification of the most effective imaging model and the efficient use of animals. This will be accomplished by weekly meetings of at least one member from each Core. These meetings will include: 1) meetings with new Pis to develop new imaging projects prior to composing new IACUC protocols, and 2) the review of completed IACUC protocols prior to IACUC submission.

UCSD放射学系已实施了一个小动物成像资源（SAIR） 支持UCSD和圣地亚哥生物搜索社区的癌症研究。 Sair位于 La Jolla的Rebecca和John Moores UCSD综合癌症中心（CC）的Vivarium 校园。该设施支持ICMIC研究人员和UCSD癌症研究的努力 调查人员，然后是校园和全市范围的努力。一些非美国联洲潜在用户包括：sidney Kimmel CC，Scripps，Burnham和Salk Institutes以及圣地亚哥生物技术公司。 UCSD SAIR将提供MRI，光学，CT，超声，高分辨率平面伽马成像，并 啮齿动物的宠物以及高分辨率的数字自身自显影和荧光成像 啮齿动物部分。除MRI外，所有模式都位于CC Vivarium附近的一个地点。支持和 专业知识包括光学成像，动物支持，图像计算，MR和光学硬件和软件， 诊断剂化学，放射化学，包括转基因，分析化学，动力学建模， 信息学，组织学和血管化实体瘤模型，以成本效益筛选成像范例。 我们的主要重点将是获取动力学建模的图像。因此，动力学 建模服务将开发自动数据减少方案。我们以前使用的经验 来自临床研究的受体密度测量的动力学建模使我们相信数据 来自图像数据集的还原路​​径必须完全自动化。造成两个原因：首先 是高吞吐量，其次也是更重要的是缺乏操作员干预措施， 消除操作员偏见。后一种质量对于严格的统计分析至关重要（通过ICMIC 成像和生化/生理指标的生物统计学服务。 高分辨率超声成像将允许量化肿瘤大小以及左测量 用于恢复PET采样输入功能的恢复校正的心室体积。最重要的是，它将允许 使用原位肿瘤模型。超声对小鼠癌症研究的关键贡献是图像的能力 以无菌的方式，无需麻醉而这样做。这应该允许经常监视 干预后的肿瘤生长或回归。 ICMIC Imaging Core的成员将与动物模型和护理的同事紧密相互作用， IMCIC生物统计学服务。我们的目标是确定最有效的成像模型， 有效使用动物。这将通过每周至少一名成员的会议来完成 每个核心。这些会议将包括：1）与新PI的会议，以开发新的成像项目 组成新的IACUC方案，以及2）在IACUC之前对完成的IACUC方案进行审查 提交。