Pediatric Biospecimen Procurement Center (BPC) Supporting the Developmental Gene Expression (dGTEx) Project

儿科生物样本采购中心 (BPC) 支持发育基因表达 (dGTEx) 项目

基本信息

批准号：
10311912
负责人：
THOMAS G BLANCHARD
金额：
$ 335万
依托单位：
NATIONAL DISEASE RESEARCH INTERCHANGE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-09 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10311912
关键词：
Address Adult Anatomy Authorization documentation Bioinformatics Brain Brain Pathology Brain imaging Caring Cells Cellular biology Child Childhood Clinical Clinical Investigator Collection Communities Data Data Analyses Data Collection Data Storage and Retrieval Development Developmental Gene Diagnosis Disease Evaluation Family Gene Expression Genetic Variation Genotype Genotype-Tissue Expression Project Grant Hospitals Human body Informatics Infrastructure Investigation Laboratories Lead Maryland Melissa Methodology Methods Modeling Molecular Molecular Profiling Organ Procurements Pathologic Pathologist Pathology Pediatric Hospitals Pediatric Research Pediatric cohort Philadelphia Physiology Population Principal Investigator Procedures Process Quality Control Recovery Reproducibility of Results Research Research Design Research Personnel Resources Risk Sampling Scientist Ships Site Specimen Structure Technical Expertise Techniques Tissue Banks Tissue Donations Tissue Sample Tissue imaging Tissues Training United States National Institutes of Health Universities biobank body system brain research data exchange design diversity and equity early childhood ethical legal social implication experimental analysis health disparity health equity improved insight medical specialties novel operation pathology imaging prepuberty programs quality assurance racial and ethnic research and development single cell analysis social culture success whole slide imaging

项目摘要

Program Director/Principal Investigator (Bell, Thomas, J.): Project Summary: Accelerating scientific discoveries across the entire spectrum of pediatric research requires advancing our current understanding of the unique molecular profiles and physiologies in all healthy tissues across the human body. The developmental Genotype-Tissue Expression (dGTEx) project will establish the first comprehensive public resource correlating gene expression and genetic variation in pediatric tissues from all major organ systems in the human body. This project will identify the molecular profiles in ~30 distinct tissue types from four developmental groups: Early post-natal, Early childhood, Pre-pubertal, and Post-pubertal. dGTEx will capitalize on new emerging methodologies to capture detailed evaluations on the developing brain and include options for single cell analysis on multiple tissues. To advance our understanding of the Ethical, Legal, Social Implications (ELSI) in pediatric tissue donation, dGTEx also includes an ELSI study to analyze these factors. The data from dGTEx will provide an unrivaled research resource for clinicians and scientists to gain new insights on the molecular milestones of regulatory processes that direct the development of healthy tissues. To support the advancement of dGTEx, this proposal will establish the pediatric Biospecimen Procurement Center (BPC) to provide the pediatric Laboratory Data Analysis Collection Center (LDACC) with suitable tissue samples for their analysis. Our proposal includes a multi-institutional effort with extensive expertise in pediatric recoveries, research, pathology, imaging, biobanking, brain research, and cutting-edge molecular techniques, such as single cell analysis. The objectives for our BPC recovery team will be led by a collaborative effort amongst: 1) National Disease Research Interchange (NDRI) to provide project management and coordinate the recovery collection effort from a network of TSS, 2) Children's Hospital of Philadelphia (CHOP) to process, store, and coordinate pathological review of all non-brain tissues and manage the bioinformatics workflow, and 3) NeuroBiobank at University of Maryland (NBB-Maryland) to process, store, and provide pathological review of brains. This breadth of expertise is essential for the BPC to provide well-annotated, suitable biospecimens to enable reliable and reproducible results from the LDACC's cutting-edge experimental methods and rigorous data interpretations. Improving our understanding of ELSI-specific challenges in pediatric tissue donation is an unmet need for the research community. To enable a more complete analysis of these challenges, Johns Hopkins All Children's Hospital (JHACH) will coordinate the dGTEx ELSI study to include evaluations of Tissue Requesters (TRs) and Family Decision Makers (FDMs) of Deceased and At-Risk Children. Collectively, our BPC team is committed to developing an all-encompassing, unparalleled BPC for the dGTEx project to empower the advancement of pediatric research, ELSI challenges, and the development of new clinical approaches to treat pediatric disorders. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page

项目总监/首席研究员（Bell, Thomas, J.）：项目概要：加速整个儿科研究领域的科学发现需要推进我们的研究目前对人类所有健康组织中独特的分子特征和生理学的了解身体。发展中的基因型组织表达 (dGTEx) 项目将建立第一个综合性的与所有主要器官的儿科组织中的基因表达和遗传变异相关的公共资源人体内的系统。该项目将鉴定来自四种组织的约 30 种不同组织类型的分子谱。发育组：产后早期、儿童早期、青春期前和青春期后。 dGTEx 将资本化关于新出现的方法来捕获对发育中大脑的详细评估，并包括以下选项：多个组织的单细胞分析。增进我们对道德、法律、社会影响的理解 (ELSI) 在儿科组织捐赠中，dGTEx 还包括一项 ELSI 研究来分析这些因素。 dGTEx 的数据将为临床医生和科学家提供无与伦比的研究资源，以获得新的见解关于指导健康组织发育的调控过程的分子里程碑。支持为了推动 dGTEx 的发展，该提案将建立儿科生物样本采购中心 (BPC)，以为儿科实验室数据分析收集中心 (LDACC) 提供合适的组织样本分析。我们的建议包括在儿科康复方面拥有广泛专业知识的多机构努力，研究、病理学、成像、生物库、脑研究和尖端分子技术，例如单细胞分析。我们的 BPC 恢复团队的目标将由以下人员的协作努力领导：1) 国家疾病研究交流中心 (NDRI) 提供项目管理并协调恢复收集 TSS 网络的努力，2) 费城儿童医院 (CHOP) 的处理、存储和协调对所有非脑组织进行病理学审查并管理生物信息学工作流程，以及 3) NeuroBiobank 马里兰大学（NBB-马里兰）处理、存储并提供大脑病理学检查。这个广度丰富的专业知识对于 BPC 提供注释良好、合适的生物样本以实现可靠且可靠的生物样本至关重要。来自 LDACC 尖端实验方法和严格数据解释的可重复结果。提高我们对儿科组织捐赠中 ELSI 特定挑战的理解是一个未得到满足的需求研究社区。为了对这些挑战进行更全面的分析，约翰霍普金斯大学所有儿童医院医院 (JHACH) 将协调 dGTEx ELSI 研究，包括对组织请求者 (TR) 和已故儿童和高危儿童的家庭决策者 (FDM)。我们的 BPC 团队共同致力于为 dGTEx 项目开发一个包罗万象、无与伦比的 BPC，以推动儿科研究、ELSI 挑战以及治疗儿科疾病的新临床方法的开发。 OMB 编号 0925-0001/0002（修订版 03/2020 已批准至 02/28/2023）页面延续格式页面