Employing smart underwear to measure gut microbial hydrogen sulfide production

利用智能内衣测量肠道微生物硫化氢的产生量

基本信息

批准号：
10425072
负责人：
Reza Ghodssi
金额：
$ 22.82万
依托单位：
UNIV OF MARYLAND, COLLEGE PARK
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-10 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10425072
关键词：
Address Affect Anogenital region Biological Availability Calories Clinical Trials Cohort Studies Collection Colorectal Cancer Complex Coupled Cysteine Devices Diet Dietary Factors Dietary Intervention Disease Enzymes Etiology Filtration Flatulence Future Gases Goals Health Human Hydrogen Hydrogen Sulfide In Vitro Intake Measurement Measures Metabolism Methane Methods Miniaturization Noise Nutrient Outcome Participant Phase Physiological Physiology Play Production Randomized Research Role Signal Transduction Signaling Molecule Source System Techniques Testing Time Ulcerative Colitis Validation arm comparison intervention design dietary experience feeding flexibility gut microbiota human microbiota metal oxide microbial microbiome miniaturized device precision nutrition prototype rectal sensor software development temporal measurement trimethylamine wearable device

项目摘要

PROJECT SUMMARY/ABSTRACT The circulating gasotransmitter H₂S is endogenously produced by tightly-regulated human enzymes and exogenously produced in a diet-dependent manner by the human microbiota. Exogenous H2S production by the human gut microbiota influences human health by modulating systemic H2S bioavailability. Excessive gut microbial H2S production is hypothesized to be involved in the etiology of colorectal cancer and Ulcerative Colitis. Our understanding of how gut microbially produced H₂S affects human health remains incomplete because current measurement techniques are imprecise, invasive, require biospecimen collection, and have limited temporal resolution. In this proposal, our objective is to address this gap by developing a research- grade wearable Smart Underwear device that enables long-term real time measurements of gut microbial H₂S production in gaseous rectal effluent (flatus). Flatus are an untapped source for measuring gut microbial H2S production. We have demonstrated that the Smart Underwear v1 prototype can precisely measure physiologically-relevant concentrations of H2S in vitro. When worn by humans, the Smart Underwear v1 prototype detects flatus with an excellent signal-to-noise ratio and is able to estimate the concentration of H2S in each flatus. In the R21 phase, we propose to develop a second revision Smart Underwear prototype for clinical trials through miniaturization, software development, and incorporation of flexible materials. In addition, we propose to conduct a user experience trial to gauge the user acceptance and test the reliability of the Smart Underwear device when worn by humans. In the R33 phase, we will use the Smart Underwear v2 prototype developed in the R21 phase to test the hypothesis that the Smart Underwear device can detect diet-induced alterations in gut microbial H2S production using a randomized controlled feeding trial that contrasts gut microbial H₂S production by modulating dietary cysteine intake. The completion of the proposed goals will lead to the first wearable device that enables long-term real time measurements of gut microbial H2S production. This will facilitate the design of personalized dietary interventions to rationally modulate gut microbial H₂S production and benefit future studies seeking to untangle the complex interactions between gut microbial H2S production and disease.

项目概要/摘要循环气体递质 H2S 是由严格调控的人体酶内源性产生的，人类微生物群以依赖饮食的方式外源产生 H2S。人类肠道微生物群通过调节肠道内过多的硫化氢生物利用度来影响人类健康。微生物产生的 H2S 被重新发现与结直肠癌和溃疡的病因有关结肠炎。我们对肠道微生物产生的硫化氢如何影响人类健康的了解仍然不完整。因为当前的测量技术不精确、具有侵入性、需要采集生物样本并且具有在这项提案中，我们的目标是通过开发一项研究来解决这一差距。级可穿戴智能内衣设备，可长期实时测量肠道微生物 H2S 直肠排气（肠胃胀气）中产生的气体是测量肠道微生物 H2S 的尚未开发的来源。我们已经证明智能内衣 v1 原型可以精确测量。人类穿着智能内衣 v1 时的 H2S 体外生理相关浓度。原型机以出色的信噪比检测肠胃气，并能够估计 H2S 的浓度在 R21 阶段，我们建议开发第二版智能内衣原型。通过小型化、软件开发和柔性材料的结合进行临床试验。我们建议进行用户体验试验，以衡量用户的接受度并测试智能的可靠性人类穿着的内衣设备在R33阶段，我们将使用智能内衣v2原型。 R21阶段开发的目的是测试智能内衣设备可以检测饮食引起的假设使用与肠道对比的随机对照喂养试验来改变肠道微生物 H2S 的产生通过调节膳食半胱氨酸摄入量来生产微生物 H2S 将导致所提出的目标的完成。首款可长期实时测量肠道微生物 H2S 产生的可穿戴设备。这将有助于设计个性化饮食干预措施，以合理调节肠道微生物 H2S 生产并有益于未来寻求解开肠道微生物 H2S 之间复杂相互作用的研究生产和疾病。