Comprehensive characterization of ancestral populations of the vector Aedes aegypti on Indian Ocean islands

印度洋岛屿媒介埃及伊蚊祖先种群的综合特征

基本信息

批准号：
10297945
负责人：
Jeffrey R POWELL
金额：
$ 67.79万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-06 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10297945
关键词：
Address Admixture Aedes Africa South of the Sahara African Arboviruses Biological Assay Blood Breeding Chikungunya virus Comparative Study Culicidae Dangerousness Data Set Dengue Detection Disease Documentation Evolution Exposure to Female Future Genetic Genetic Databases Genetic Variation Genome Genomics Goals Habitats Human Indian Ocean Indian Ocean Islands Infection Insecta Island Laboratories Madagascar Mammals Methods Microfluidics Morphology Names Natural History Phylogenetic Analysis Population Population Genetics Protocols documentation RNA RNA Viruses Recording of previous events Research Resources Reverse Transcriptase Polymerase Chain Reaction Sister Site Source Surveys System Taxon Taxonomy Viral Virus Work Yellow Fever ZIKA base chikungunya disorder risk experimental study forest human disease human pathogen insight member multidisciplinary novel particle population genetic structure reproductive trait transcriptome sequencing vector vector competence virome

项目摘要

Project Summary/Abstract We have recently shown that the ancestors of all Aedes aegypti populations in both sub-Saharan Africa as well as around the world presently reside on islands in the Southwest Indian Ocean (SWIO) including Madagascar. These islands gave rise to continental African Ae. aegypti formosus no earlier than about 100,000 years ago. We propose multidisciplinary studies on SWIO populations of Ae. aegypti along with its close relatives, Ae. mascarensis and Ae. pia. This will include population genetics, phylogenetics/phylogeography, vector competence, blood meal analyses, and characterization of the virome of field-caught females. We address question such as: Are the ancestors that gave rise to Ae. aegypti s.s. capable of transmitting the same viruses that cause human diseases? Are these mosquitoes carrying the same viruses in the field and/or do they harbor novel undescribed arboviruses? What are the sources of their blood meals? Where do they breed? Population genetics and phylogenetic preliminary work have already identified candidates for new undescribed species and we suspect more cryptic taxa will be found. We propose to assemble complete genomes for the most informative populations/taxa that will allow comparative studies as well as inference of the ancestral genome of Ae. aegypti s.s., a fundamental resource for analysis of a plethora of genomics studies ongoing in laboratories around the world.

项目概要/摘要我们最近表明，撒哈拉以南非洲地区所有埃及伊蚊种群的祖先目前世界各地都居住在西南印度洋 (SWIO) 的岛屿上，包括马达加斯加。这些岛屿孕育了非洲大陆的伊蚊。埃及伊蚊的出现时间不早于大约 10 万年前。我们建议对白伊蚊 SWIO 群体进行多学科研究。埃及伊蚊及其近亲伊蚊。 mascarensis 和 Ae.皮亚。这将包括群体遗传学、系统发育学/系统发育地理学、载体现场捕获的雌性的能力、血粉分析和病毒组特征。我们解决诸如此类的问题：Ae 的祖先是产生的吗？埃及伊蚊号能够传播相同的病毒会导致人类疾病吗？这些蚊子在野外是否携带相同的病毒和/或它们是否携带相同的病毒？隐藏着未描述的新型虫媒病毒？他们的血粉的来源是什么？它们在哪里繁殖？群体遗传学和系统发育的初步工作已经确定了新的未描述的候选者物种，我们怀疑会发现更多神秘的类群。我们建议组装完整的基因组信息最丰富的种群/分类单元，可以进行比较研究以及祖先的推断 Ae 基因组。 aegypti s.s.，用于分析正在进行的大量基因组学研究的基本资源世界各地的实验室。