Core B: Reagent/Service Core

核心 B：试剂/服务核心

基本信息

批准号：
10229416
负责人：
Eric Moon-shong M. TANG
金额：
$ 25.39万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-12 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10229416
关键词：
Animal Model Animals Antibodies Bladder Bladder Tissue Cell Line Collaborations Common Core Complementary DNA Consult Consultations Data Databases Deposition Emergency Situation Ensure Environmental Medicine Equipment and supply inventories Event Experimental Designs Genetic Genetically Engineered Mouse Genitourinary system Human Hurricane In Vitro Individual Knock-out Laboratories Link Malignant neoplasm of urinary bladder Molecular Mus Mutation Nucleic Acids Pathologic Pathologist Pathology Plasmids Process Program Research Project Grants Proteins Quality Control Reagent Reporting Reproducibility Research Research Personnel Research Project Grants Research Support Resource Sharing Resources Sampling Services Site Source Specimen Statistical Data Interpretation Time Tissue Model Tissue Preservation Tissue Sample Tissues Training Training Support Variant Work base cancer cell cohesion cost effective cost effectiveness design experience experimental study in vivo molecular subtypes mouse model muscle invasive bladder cancer non-muscle invasive bladder cancer population health professor programs repository small hairpin RNA statistics success synergism time use tool tumorigenesis vector

项目摘要

Core B Summary The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder tumorigenesis of both major bladder cancer (BC) variants and molecular subtypes. In addition to the intellectual and experimental synergies that link the individual projects into a cohesive program, each of the three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different grades and stages of major BC variants and molecular subtypes, unique mouse models of bladder cancer developed/used by project leaders Dr. Wu, Dr. Tang and Dr. Huang, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would it be an efficient use of time, money, and resources for each laboratory to independently generate, procure, characterize, and maintain all these reagents. Instead, we have established a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, mouse tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the project directors and guards against accidental loss of invaluable reagents in emergency situations such as power outages (which we last experienced in 2012 during Sandy Superstorm). Finally, the Core B will also provide pathology and statistical support for the individual projects. The specific aims of the Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects; ii) To collect mouse specimens and provide the necessary pathology support service; and iii) To provide dedicated statistical support. Success of this Core will be an essential factor for the studies that have been proposed in this program project.

核心B总结组成该计划项目的三个研究项目都专注于了解分子以及导致两种主要膀胱癌（BC）膀胱肿瘤发生的细胞事件和机制变体和分子亚型。除了将知识和实验联系起来的协同作用之外将各个项目整合为一个有凝聚力的计划，三个拟议的研究计划中的每一个都取决于一个为他们的成功持续提供一套通用的关键试剂和服务。这些包括但不限于代表主要 BC 不同等级和阶段的一组培养细胞系项目负责人开发/使用的膀胱癌变异和分子亚型、独特的小鼠模型吴博士、唐博士和黄博士，核酸试剂、抗体和 cDNA 构建体。显然，这会没有意义，也不是每个实验室有效利用时间、金钱和资源的方法独立生成、采购、表征和维护所有这些试剂。相反，我们建立了集中式试剂和服务核心，其目的之一是作为密钥的中央存储库试剂并为这些重要的研究工具提供中央供应，所有参与者都可以共享本计划项目。除了成本效益之外，这种方法还具有确保各个项目中这些共享试剂的质量。提供细胞系、小鼠组织供应来自集中核心的动物模型消除了试剂处理的可变性，促进了数据的获取三个单独项目的比较。此外，它还促进了项目主管并防止在紧急情况下意外丢失宝贵的试剂，例如停电（我们上次遇到停电是在 2012 年桑迪超级风暴期间）。最后，Core B 还将为各个项目提供病理学和统计支持。核心的具体目标是： i) 维持集中供应并分配对支持研究工作至关重要的一组共同核心试剂各个组成项目； ii) 采集小鼠标本并提供必要的病理学支持服务; iii) 提供专门的统计支持。该核心的成功将是本计划项目中提出的研究。