Assays and kits for MSC-derived extracellular vesicles

MSC 衍生的细胞外囊泡的检测方法和试剂盒

• 批准号: 10227192
• 负责人: JOHN P NOLAN
• 金额: $ 89.09万
• 依托单位: CELLARCUS BIOSCIENCES INC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227192
• 关键词: Address; Adipose tissue; Affect; Alleles; Antibodies; Antibody Affinity; Antibody Specificity; Automation; Biological Assay; Biological Sciences; Biotechnology; Bone Marrow; Cell Culture Techniques; Cell Therapy; Cells; Client; Clinical; Clinical Trials; Color; Controlled Environment; Data; Development; Disease; Distant; Dose; Engineering; Feedback; Flow Cytometry; Fluorescent Antibody Technique; Heterogeneity; Immunoassay; International; Laboratories; Measurement; Measures; Membrane Proteins; Monitor; Monoclonal Antibodies; Optics; Organ; Performance; Pharmacologic Substance; Phase; Preparation; Production; Property; Protocols documentation; Reagent; Regenerative Medicine; Reporting; Reproducibility; Research; Research Personnel; Resolution; Role; Safety; Sampling; Services; Societies; Standardization; Surface; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Training; Umbilical cord structure; Vesicle; Work; analytical method; biomarker panel; design; detection limit; dosage; extracellular vesicles; in vitro activity; in vivo; interest; liquid biopsy; mesenchymal stromal cell; novel therapeutics; potential biomarker; reagent standard; regenerative; regenerative cell; regenerative therapy; tool; vesicular release

Abstract All cells release small extracellular vesicles (EVs) that can carry cargo to other cells to affect their function. Mesenchymal stromal cells (MSCs) release EVs that have regenerative activities in vitro and in vivo and which represent potentially valuable new therapeutics. The realization of this potential is hindered by a lack of analytical methods to characterize MSC EVs, determine their mechanisms of action, and establish potency and dosage criteria. We will produce high resolution analysis tools for MSC EVs that will address these limitations and enable their development as new therapeutic entities.

抽象的 所有细胞都会释放小的细胞外囊泡（EV），这些囊泡可以将货物运送到其他细胞以影响 他们的功能。间充质基质细胞 (MSC) 释放具有再生活性的 EV 体外和体内，代表潜在有价值的新疗法。的实现 由于缺乏表征 MSC EV 的分析方法、确定 他们的作用机制，并建立效力和剂量标准。我们将生产高 用于 MSC EV 的分辨率分析工具将解决这些限制并使其能够 开发为新的治疗实体。

项目成果

Rigor and reproducibility: status and challenges for single vesicle analysis.

严谨性和重现性：单囊泡分析的现状和挑战。

• 发表时间: 2022
• 作者: Nolan, John P;Chiu, Daniel T;Welsh, Joshua A
• 通讯作者: Welsh, Joshua A