Core--Scientific

核心--科学

• 批准号: 7615142
• 负责人: BRADLEY T. HYMAN
• 金额: $ 25.28万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7615142
• 关键词: Arts; Blood - brain barrier anatomy; Blood flow; Cerebrovascular Circulation; Contrast Media; Core-Binding Factor; Data; Equipment; Extravasation; Fluorescence; Functional Magnetic Resonance Imaging; Hemoglobin; Image; Laboratories; Lasers; Magnetic Resonance Imaging; Matrix Metalloproteinases; Measurement; Measures; Methodology; Plasma; Research Personnel; Rodent; Techniques; base; design; in vivo; member; programs; Arts; Blood - brain barrier anatomy; Blood flow; Cerebrovascular Circulation; Contrast Media; Core-Binding Factor; Data; Equipment; Extravasation; Fluorescence; Functional Magnetic Resonance Imaging; Hemoglobin; Image; Laboratories; Lasers; Magnetic Resonance Imaging; Matrix Metalloproteinases; Measurement; Measures; Methodology; Plasma; Research Personnel; Rodent; Techniques; base; design; in vivo; member; programs

The scientific core consists of two major imaging laboratories to support program project members with state of the art equipment and methodologies. Aim 1. Provide support to investigators to design and carry out anatomically based analyses utilizing quantitative neuroanatomical techniques including stereology based statistically unbiased approaches. Aim 2. Assist investigators in imaging blood brain barrier breakdown and MMP uregulation, both in vivo and ex vivo, using extravasation of fluorescent indicators. Aim 3. Assist investigators in utilizing laser speckle contrast imaging in studies of cerebral blood flow and in the application of multi-spectral in-vivo imaging of intrinsic (e.g hemoglobin concentrations) and extrinsic (e.g. plasma leakiness indicators and MMP activatable fluorescence markers) contrast agents. Advance the methodology to provide more robust measures of blood flow and facilitate investigator driven advanced analyses. Aim 4. Develop robust high-field MRI measurements of CBF in rodents, and assist investigators in the acquisition and analysis of anatomical and functional MRI data. Taken together, these aims both support the scientific aims of the program project as a whole, while the needs of the projects serve to advance the methodology to facilitate investigator driven analyses.

科学核心由两个主要成像实验室组成，以支持计划成员具有最先进的设备和方法论。 AIM 1。为研究人员提供支持，以设计和进行基于解剖学的分析，利用定量神经解剖技术，包括基于立体的统计学上无偏见的方法。 AIM 2。使用荧光指示剂的渗出，协助研究人员在体内和体内成像血液屏障的崩溃和MMP渗透。 AIM 3。协助研究人员利用激光斑点对比成像在脑血流的研究和应用中 固有（例如血红蛋白浓度）和外部（例如等离子体泄漏指标和MMP可激活荧光标记物）对比度的多光谱体内成像。推进该方法，以提供更强大的血流测量，并促进研究者驱动的高级分析。目标4。在啮齿动物中开发CBF的强大高场MRI测量值，并协助研究人员对解剖和功能MRI数据的获取和分析。综上所述，这些目标都支持整个计划项目的科学目标，而项目的需求则可以推进方法论，以促进研究者驱动的分析。