Micrarray Core

微阵列核心

• 批准号: 7626840
• 负责人: DONALD A BALDWIN
• 金额: $ 19.38万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7626840
• 关键词: Address; Bioinformatics; Biological Assay; Biological Process; Collaborations; Collection; Core Facility; Custom; Data; Data Analyses; Depth; Development; Disadvantaged; Environment; Evaluation; Faculty; Funding; Genes; Genetic; Genomics; Glass; Goals; Housing; Knowledge; Location; Methods; Molecular; Musculoskeletal; Musculoskeletal Diseases; Oligonucleotide Microarrays; Pennsylvania; Performance; Printing; Process; Proteins; Protocols documentation; RNA; Range; Regulation; Regulatory Element; Research; Research Personnel; Services; Slide; System; Techniques; Tissues; Training; Transcript; Universities; Variant; cost; functional genomics; injury and repair; innovation; instrumentation; medical schools; novel; programs; tool

The musculoskeletal research field is becoming increasingly focused on studies at the genetic level. In particular, a variety of evaluation tools have recently been developed that allow investigators to understand the molecular systems in which biological processes occurs. The field of functional genomics seeks to annotate genomic sequence with assignments of gene and regulatory element identities and locations, RNA variants and abundance levels, and protein variants, abundances and interactions. As functional annotations accumulate, detailed and in-depth studies of specific genes and their products can be placed into a genomic context to reveal the molecular systems. In turn, those systems and processes can be studied using functional genomics techniques to identify the genes important for their regulation. One such technique is transcript profiling, the parallel quantitation of RNA abundance for thousands of genes. Transcript profiling can be open-ended, using methods that do not rely on previous knowledge of gene sequences, or closed to a defined set of probes for known RNAs. The Penn Microarray Facility provides instrumentation and expertise for RNA transcript profiling. The overall objective of this Microarray Core Facility is to develop and utilize genomic analyses directed toward problems of musculoskeletal tissue injury and repair. The Specific Aims are to: 1) provide guidance and training on the capabilities, advantages, and disadvantages, of various genomic protocols and analyses for musculoskeletal research through formal educational enrichment programs and through one-on-one interactions, 2) provide expertise and service for Affymetrix GeneChip assays, 3) provide expertise and service for the development and use of custom printed microarrays, 4) facilitate access to training and bioinformatics tools appropriate for analyzing the data produced in Aims 2 and 3, and 5) provide funding for development of new projects and collaborations and to develop preliminary and/or feasibility data for investigators. Successful completion of these aims will significantly enhance the environment and the capabilities of researchers at the University of Pennsylvania, leading to the development of novel and innovative approaches to musculoskeletal disorders.

肌肉骨骼研究领域越来越重视在遗传水平的研究上。 特别是，最近已经开发了各种评估工具，使研究人员得以 了解生物过程发生的分子系统。功能领域 基因组学试图通过基因和调节元件的分配来注释基因组序列 身份和位置，RNA变体和丰度水平以及蛋白质变异，丰度和 互动。随着功能注释的积累，对特定基因的详细和深入研究 他们的产品可以放入基因组环境中以揭示分子系统。反过来， 可以使用功能基因组技术来研究这些系统和过程 基因对它们的调节很重要。一种这样的技术是笔录分析，平行 对数千个基因的RNA丰度的定量。笔录分析可以是开放式的， 使用不依赖基因序列知识或封闭定义的方法 一组已知RNA的探针。 Penn微阵列设施提供仪器和 RNA成绩单分析的专业知识。此微阵列核心设施的总体目标是 开发和利用针对肌肉骨骼组织损伤问题的基因组分析和 维修。具体目的是：1）提供有关功能，优势， 以及各种基因组方案的缺点和肌肉骨骼研究的分析 通过正式的教育丰富计划和一对一的互动，2） Affymetrix Genechip分析的专业知识和服务，3）为 定制印刷微阵列的开发和使用，4）促进培训的访问和 生物信息学工具适合分析目标2和3中产生的数据，以及5） 资金用于开发新项目和合作，并开发初步和/或 研究人员的可行性数据。这些目标的成功完成将大大增强 宾夕法尼亚大学的环境和研究人员的能力，导致 开发肌肉骨骼疾病的新颖和创新方法。