INTEGRATED MICROSCOPY

集成显微镜

• 批准号: 7714285
• 负责人: BENJAMIN S GLICK
• 金额: $ 7.15万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-22 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714285
• 关键词: Area; Arts; Biochemical; Cancer Center Support Grant; Cells; Collaborations; Color; Communities; Computer software; Computers; Condition; Data; Decision Making; Dimensions; Equipment; Fluorescence; Fluorescence Microscopy; Fostering; Funding; Generations; Hour; Human Resources; Image; Image Analysis; Imaging technology; Individual; Knowledge; Laboratories; Learning; Life; Methods; Microscope; Microscopy; Mission; Operative Surgical Procedures; Optics; Peer Review; Physiological; Pilot Projects; Population; Preparation; Publications; Qualifying; Range; Reliance; Reporting; Research; Research Personnel; Resolution; Resources; Running; Services; Speed; Staining method; Stains; Sum; System; Techniques; Technology; Testing; Time; Training; Universities; University of Chicago Cancer Research Center; Update; Work; anticancer research; base; cost; design; desire; improved; instrument; instrumentation; member; millimeter; nanometer; programs; research study; tool; wasting

The Integrated Microscopy Facility (IMF) functions as a supervised, user-based Core providing state-of-theart microscopy imaging capabilities to UCCRC members and other University investigators. The primary mission of the Facility is to provide a valuable, unique service to the University community by furnishing highquality microscopy instrumentation, image analysis tools, and expert assistance. In addition, maintaining equipment in optimal condition and training users require skilled technical personnel, something that individual laboratories typically cannot afford. Because imaging technology and computational capacity are evolving so rapidly, it is extremely difficult for individual researchers to maintain or afford cutting-edge capabilities. Integrated Microscopy offers one of the most flexible and informative tools available to researchers, offering scales of detail ranging from tens of millimeters to less than 10 nanometers with appropriate techniques, Available techniques provided by the IMF range from classic color histological stain imaging, to contrast generation in unstained cells, to fluorescence technologies. Fluorescence methods continue to offer the greatest range of applications ranging from localization of multiple targets to readout of biochemical or physiologic parameters in either fixed or living preparations. Accordingly, the greatest resources of the IMF are allocated to fluorescence microscopy. By merging the microscopy facilities created by several campus entities, the combined pool of resources is more efficiently managed and maintained, while also increasing access, capabilities, and capacity for UCCRC member research. Overall use of the IMF has increased close to three times the activity reported in the 2001 CCSG submission. Over 63 peer-reviewed investigators across six Scientific Programs routinely use the IMF, totaling 49% of Facility usage. Services provided by the IMF consist of the following: ¿ Provide state-of-the-art microscopy imaging capabilities including brightfield (color, DIG, etc), fluorescence (multi-dimension; TIRFM; bleaching, etc), high-resolution, -speed, -sensitivity, confocal, and physiologic techniques. ¿ Provide training on all instruments and expert advice to investigators regarding experiment design and execution. Provide oversight to assure proper equipment use and minimize breakage. ¿ Provide computers including networked data servers and software for image analysis and publication. ¿ Serve as an educational resource for University investigators in the use of current microscopy techniques and foster interactions among users.

集成显微镜设施（IMF）充当有监督的，基于用户的核心，提供最先进的核心 UCCRC成员和其他大学研究人员的显微镜成像功能。主要 该设施的使命是通过提供高质量的大学社区为大学社区提供宝贵的独特服务 显微镜仪器，图像分析工具和专家帮助。另外，维护 处于最佳状态和培训用户的设备需要熟练的技术人员，这是 个别实验室通常负担不起。因为成像技术和计算能力是 进化如此迅速，个人研究人员很难维持或负担前沿 功能。 综合显微镜提供了研究人员可用的最灵活，最有用的工具之一，提供 细节范围从数十毫米到少于10纳米的范围，并具有适当的技术， IMF提供的可用技术从经典的颜色组织学染色成像到对比 在未染色的细胞中产生，荧光技术。荧光方法继续提供 最大的应用程序范围从多个目标的本地化到生化或 固定或生活制剂中的生理参数。根据每个国际货币基金组织的最大资源 分配给荧光显微镜。通过合并几个校园创建的显微镜设施 实体，合并的资源池更有效地管理和维护，同时也增加了 UCCRC成员研究的访问，能力和能力和能力。 IMF的总体使用已增加 在2001年CCSG提交中报告的活动的三倍。超过63个同行评审的调查员 在六个科学计划中，通常使用IMF，总计49％的设施使用情况。 IMF提供的服务包括以下内容： �提供最新的显微镜成像功能，包括Brightfield（颜色，挖掘等）， 荧光（多维； TIRFM；漂白等），高分辨率， - 速度， - 敏感性，共同点， 和生理技术。 �向调查人员提供有关实验设计的所有工具和专家建议的培训 和执行。提供监督以确保适当的设备使用并最大程度地减少断裂。 »提供计算机，包括网络数据服务器和用于图像分析和发布的软件。 „是大学研究人员使用当前显微镜的教育资源 用户之间的技术和寄养互动。