MONOCLONAL ANTIBODY

单克隆抗体

• 批准号: 7671820
• 负责人: LISA K. DENZIN
• 金额: $ 16.49万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7671820
• 关键词: Antigens; Biomedical Research; Core Facility; Diagnosis; Disease; Enzymes; Generations; Goals; Hybridomas; In Vitro; Infection; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Modification; Monoclonal Antibodies; Mycoplasma; Production; Proteins; Recombinants; Research Personnel; Sampling; Services; Support of Research; Testing; cost; interest; programs; tissue culture; tool

The Monoclonal Antibody Core Facility (MACF) provides the following four high quality services not only at a reasonable cost to investigators but also in a timely manner. 1. The generation of new MAbs / hybridomas to a specific antigen of interest. 2. MAb production (in vitro), purification and modification (including conjugation to fluorphores, biotion and enzymes and the fragmentation of MAbs). 3. Mycoplasma testing of tissue culture samples. 4. In vitro production of recombinant/secreted proteins. Monoclonal antibodies (MAbs) are essential tools used in most if not all fields of biomedical research, in the diagnosis of diseases, and in the treatment of diseases such as infections and cancer. The goal of the MACF is to support the research interests of MSKCC investigators by providing key services related to the production, purification and use of MAbs. Over the last 5 years demand for the MACF services has remained consistently high. From July 2005 through July 2006, 61 investigators from 9 out of 10 programs placed orders with the facility for any combination of the services noted above.

单克隆抗体核心设施（MACF）不仅提供以下四项优质服务 调查人员的费用合理，而且及时。 1.针对感兴趣的特定抗原产生新的单克隆抗体/杂交瘤。 2. MAb 生产（体外）、纯化和修饰（包括与荧光团缀合， 生物素和酶以及单克隆抗体的碎片）。 3.组织培养样品的支原体检测。 4.重组/分泌蛋白的体外生产。 单克隆抗体 (MAb) 是大多数（如果不是全部）生物医学研究领域中使用的重要工具。 疾病诊断以及感染和癌症等疾病的治疗。的目标 MACF 将通过提供与 MSKCC 相关的关键服务来支持 MSKCC 研究人员的研究兴趣 MAb 的生产、纯化和使用。 过去 5 年，对 MACF 服务的需求一直保持较高水平。 2005年7月起 截至 2006 年 7 月，来自 10 个项目中 9 个项目的 61 名调查员向该设施下达了任何 上述服务的组合。