Population cytogenetics of Anopheles moucheti and Anopheles nili

穆切按蚊和尼利按蚊的群体细胞遗传学

• 批准号: 7650165
• 负责人: IGOR V SHARAKHOV
• 金额: $ 21.97万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-03 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7650165
• 关键词: Africa; African; Anopheles Genus; Anopheles gambiae; Attention; Cameroon; Cells; Chromosome inversion; Chromosomes; Culicidae; Cytogenetic Map; Cytogenetics; DNA biosynthesis; Development; Drosophila genus; Effectiveness; Electron Microscopy; Frequencies; Genes; Genetic Polymorphism; Genetic Population Study; Genetic Recombination; Genome; In Situ Hybridization; Location; Malaria; Maps; Mediating; Methods; Microsatellite Repeats; Nurses; Organism; Ovarian; Pattern; Physical Map of the Human Genome; Plasmodium falciparum; Population; Population Study; Preparation; Primed In Situ Labeling; Process; Protocols documentation; Public Health; Research; Resolution; Sampling; Social Welfare; Structure; Testing; Time; Tissues; base; improved; innovation; light microscopy; member; molecular marker; neglect; next generation; novel; population genetic structure; pressure; public health relevance; tool; vector; vector control

DESCRIPTION (provided by applicant): Malaria has a devastating impact on public health and welfare in the African continent. An important approach for reducing or eliminating malaria impact is vector control. However, vector control is inefficient if not all major vector species are targeted. Mosquitoes of subgenus Cellia - Anopheles gambiae, An. arabiensis, An. funestus, An. moucheti, and An. nili - are the major malaria vectors because they are anthropophilic and susceptible to Plasmodium falciparum. An. gambiae, An. arabiensis, and An. funestus are being intensively studied with the help of cytogenetic and molecular markers. An. moucheti and An. nili have received significantly less attention because the tools needed to precisely characterize species diversity and population structure are lacking. This proposal offers a unique opportunity to develop cytogenetic research tools and to assess their effectiveness for taxonomic and population genetics studies of the neglected malaria vectors An. moucheti and An. nili. The commonly used markers for inferring anopheline taxonomic and population status include chromosomal inversions and microsatellites. Reduced recombination and selection can influence loci within inversions or near inversion breakpoints, resulting in estimates of gene flow that may depart significantly from those based on loci elsewhere in the genome. Therefore, the physical location of microsatellite markers with respect to polymorphic inversions is important information for interpreting population genetic structure. Innovative approaches will be used for developing next-generation-quality cytogenetic and physical maps for malaria vectors. In addition to the traditional cytogenetic and mapping protocols, the project will utilize a novel high-pressure method to prepare polytene chromosome spreads and the primed in situ labeling (PRINS), which is based on a primer-mediated DNA synthesis and used on chromosomes of various organisms. Integrated maps of polytene chromosomes, inversion breakpoints, and microsatellite markers will revolutionize population studies of the neglected malaria vectors. Using these maps, the proposed project will test a hypothesis that there are inversion differences among members of the An. moucheti and An. nili groups. Briefly, the specific aims of this R21 proposal are to 1. Develop high-resolution cytogenetic photomaps for the malaria vectors An. moucheti and An. nili. 2. Determine the taxonomic status and population structure of members of the An. moucheti and An. nili groups using fixed and polymorphic inversions. 3. Identify the chromosomal locations of microsatellite markers for An. moucheti and An. nili with respect to polymorphic inversions. PUBLIC HEALTH RELEVANCE Malaria has a devastating impact on public health and welfare in the African continent. An important approach for reducing or eliminating malaria impact is vector control. This proposal offers a unique opportunity to develop cytogenetic research tools and to assess their effectiveness for taxonomic and population genetics studies of the neglected malaria vectors An. moucheti and An. nili.

描述（由申请人提供）：疟疾对非洲大陆的公共卫生和福利有毁灭性的影响。减少或消除疟疾影响的重要方法是载体控制。但是，即使不是所有主要的矢量物种，也不是所有主要的矢量物种效率低下。亚属细胞菌的蚊子 - 冈比亚河流，an。阿拉伯语，一个。 Funestus，一个。 moucheti和。尼利 - 是主要的疟疾载体，因为它们是拟人化的并且容易受到恶性疟原虫的影响。一个。冈比亚，一个。阿拉伯语和。在细胞遗传学和分子标记的帮助下，正在深入研究Funestus。一个。 moucheti和an。尼利（Nili）受到明显的关注，因为缺乏精确表征物种多样性和种群结构所需的工具。该建议提供了一个独特的机会来开发细胞遗传学研究工具，并评估其对被忽视的疟疾载体的分类学和人群遗传学研究的有效性。 moucheti和an。尼利。用于推断瞬时分类学和人口状态的常用标记包括染色体反演和微卫星。减少的重组和选择会影响反转或近倒转断点内的基因座，从而导致基因流的估计值可能与基于基因组其他地方的基因座显着不同。因此，微卫星标记在多态性倒置方面的物理位置是解释种群遗传结构的重要信息。创新方法将用于开发疟疾载体的下一代质量细胞遗传学和物理图。除了传统的细胞遗传学和映射方案外，该项目还将利用一种新型的高压方法来制备多烯染色体扩散和原位标记（PRINS），该方法基于引物介导的DNA合成并用于各种生物体的染色体上。聚单位染色体，反转断点和微卫星标记的综合图将彻底改变被忽视的疟疾载体的人群研究。使用这些地图，提出的项目将检验一个假设，即AN成员之间存在反演差异。 moucheti和an。尼利组。简而言之，该R21提案的具体目的为1。 moucheti和an。尼利。 2。确定AN成员的分类状态和人口结构。 moucheti和an。 NILI组使用固定和多态性反转。 3。确定An的微卫星标记的染色体位置。 moucheti和an。 NILI相对于多态性反转。公共卫生相关性疟疾对非洲大陆的公共卫生和福利有毁灭性的影响。减少或消除疟疾影响的重要方法是载体控制。该建议提供了一个独特的机会来开发细胞遗传学研究工具，并评估其对被忽视的疟疾载体的分类学和人群遗传学研究的有效性。 moucheti和an。尼利。

项目成果

Cryptic genetic diversity within the Anopheles nili group of malaria vectors in the equatorial forest area of Cameroon (Central Africa).

• DOI: 10.1371/journal.pone.0058862
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ndo C;Simard F;Kengne P;Awono-Ambene P;Morlais I;Sharakhov I;Fontenille D;Antonio-Nkondjio C
• 通讯作者: Antonio-Nkondjio C

Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes.

• DOI: 10.1186/1756-3305-4-202
• 发表时间: 2011-10-19
• 期刊: Parasites & vectors
• 影响因子: 3.2
• 作者: Peery A;Sharakhova MV;Antonio-Nkondjio C;Ndo C;Weill M;Simard F;Sharakhov IV
• 通讯作者: Sharakhov IV

Cytogenetic analysis of Anopheles ovengensis revealed high structural divergence of chromosomes in the Anopheles nili group.

• DOI: 10.1016/j.meegid.2013.03.010
• 发表时间: 2013-06
• 期刊: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
• 作者: Sharakhova MV;Peery A;Antonio-Nkondjio C;Xia A;Ndo C;Awono-Ambene P;Simard F;Sharakhov IV
• 通讯作者: Sharakhov IV

Multigene phylogenetics reveals temporal diversification of major African malaria vectors.

• DOI: 10.1371/journal.pone.0093580
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kamali M;Marek PE;Peery A;Antonio-Nkondjio C;Ndo C;Tu Z;Simard F;Sharakhov IV
• 通讯作者: Sharakhov IV

Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa.

• DOI: 10.1111/mve.12037
• 发表时间: 2014-09
• 期刊: Medical and veterinary entomology
• 影响因子: 1.9
• 作者: Sharakhova MV;Antonio-Nkondjio C;Xia A;Ndo C;Awono-Ambene P;Simard F;Sharakhov IV
• 通讯作者: Sharakhov IV