Early life metal exposure, the gut microbiome, and neurodevelopment in childhood

生命早期的金属暴露、肠道微生物组和儿童期的神经发育

基本信息

批准号：
10371388
负责人：
Shoshannah Iylene Eggers
金额：
$ 10.52万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-12-16 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10371388
关键词：
11 year old Address Adolescence Age Area Attention Behavioral Biological Biological Markers Biology Biometry Birth Blood Brain Chemical Exposure Chemicals Childhood Cities Clinical Collaborations Copper Cross-Sectional Studies Data Data Analyses Development Environment Environmental Epidemiology Environmental Exposure Environmental Health Environmental Microbiology Epidemiologist Faculty Feces Foundations Funding Future Goals Growth Hair Health Human Human Microbiome Hydrocortisone Individual Infant Intervention Knowledge Laboratories Lead Learning Life Link Manganese Measurement Measures Mediation Mentors Mentorship Metabolic Metagenomics Metal exposure Metals Methodology Mexico Modeling National Institute of Environmental Health Sciences Neurologic Neurological outcome Obesity Observational Study Pathway interactions Play Positioning Attribute Predisposition Proteobacteria Protocols documentation Research Research Activity Research Personnel Research Proposals Research Training Resolution Role Rotation Second Pregnancy Trimester Secure Shapes Shotguns Time Tooth structure Training Training Activity Walkers Work Zinc base behavior measurement career career development cohort early detection biomarkers early life exposure epidemiology study executive function fetal gut bacteria gut microbiome gut-brain axis infancy innovation interest meetings metabolome metabolomics metagenomic sequencing microbial microbiome microbiome analysis microbiome composition microbiome research multidimensional data multidisciplinary multiple omics neurobehavioral neurodevelopment neurotoxicology novel pilot test prenatal prenatal exposure prospective research and development sample collection skills social stressor stool sample success symposium tenure track time use

项目摘要

PROJECT SUMMARY I am an environmental epidemiologist and microbiome researcher with a primary research interest in the role of the human microbiome between environmental exposures and human health, including neurological outcomes. The goal of this proposal is to obtain training and acquire the skills needed to continue to build my academic research career by linking early life metal exposure, the gut microbiome and metabolome in childhood, and neurodevelopmental measures of attention. For this proposal, I plan to train with an expert team of mentors, collaborators, and advisors with transdisciplinary expertise in exposomics, exposure biology, neurodevelopment, biostatistics, metabolomics, epidemiology, and microbiome research. The proposed training plan includes clinical and laboratory rotations, coursework, seminars and meetings, conferences, and 1-on-1 tutorials with mentors and advisors, covering research and career development topics. This training will provide skills and knowledge in the areas of neurodevelopment, and exposomics, including exposure biology and multi-omics analysis. Upon completion of this training plan, I will be able to achieve my long-term career goal of becoming an independent researcher focused on the inter-relationships among environmental exposures, the microbiome, and neurodevelopment. To that end, I propose leveraging the use of the established Programming Research in Obesity, GRowth, Environment and Social Stressors (PROGRESS) birth cohort, based in Mexico City, and adding microbiome analysis. Specifically, I will: 1) train with Dr. Arora in exposure biology and analysis of baby teeth as a biomarker of early life metal exposure; 2) train with Dr. Wright, in collaboration with Dr. Shamesh, in neurodevelopmental assessment, principals of neurotoxicology, and their use in epidemiologic studies; 3) train with Dr. Faith in metagenomic analysis, 4) integrate biostatistical assessment of mixtures and high dimensional data, guided by Dr. Gennings; 4) learn about stool metabolomic analysis methodology with Dr. Walker; 5) work with Dr. Tellez-Rojo to facilitate stool sample collection and data analysis in PROGRESS. I will use these training and research activities to integrate the microbiome into an exposomic analysis of neurodevelopment. I will use the research and career development training I receive to establish myself as an independent investigator with a tenure track faculty position, able to secure R01 funding. This research proposal combines innovative measures of exposure, novel data analysis approaches, and high resolution omics analysis to address gaps in the understanding of timing and potential mechanisms by which metal exposures, and the gut microbiome and metabolome, are associated with neurodevelopmental measures of attention. The proposed research and training plan builds the foundation for an independent research career that aims to clarify the links between environmental exposures and neurodevelopment, via the human microbiome.

项目概要我是一名环境流行病学家和微生物组研究员，主要研究兴趣是环境暴露与人类健康（包括神经系统结果）之间的人类微生物组。该提案的目标是获得培训并获得继续发展学术所需的技能通过将生命早期的金属暴露、儿童时期的肠道微生物组和代谢组联系起来进行研究生涯，以及注意力的神经发育测量。对于这个提案，我计划与一个由导师、合作者和顾问组成的专家团队一起进行培训暴露组学、暴露生物学、神经发育、生物统计学、代谢组学等跨学科专业知识，流行病学和微生物组研究。拟议的培训计划包括临床和实验室轮转，课程作业、研讨会和会议、大会以及导师和顾问的一对一教程，涵盖研究和职业发展主题。该培训将提供以下领域的技能和知识神经发育和暴露组学，包括暴露生物学和多组学分析。完成后通过这个培训计划，我将能够实现成为一名独立研究员的长期职业目标重点关注环境暴露、微生物组和神经发育之间的相互关系。为此，我建议利用已建立的肥胖、生长、生长等方面的规划研究，环境和社会压力源 (PROGRESS) 出生队列，位于墨西哥城，并添加了微生物组分析。具体来说，我将： 1) 与 Arora 博士一起接受暴露生物学和乳牙分析方面的培训，作为一名生命早期金属暴露的生物标志物； 2) 与 Wright 博士一起与 Shamesh 博士合作进行培训神经发育评估、神经毒理学原理及其在流行病学研究中的应用； 3）火车与 Faith 博士一起进行宏基因组分析，4) 整合混合物和高维的生物统计评估数据，由 Gennings 博士指导； 4) 跟随Walker博士学习粪便代谢组学分析方法； 5）工作与 Tellez-Rojo 博士合作，促进粪便样本收集和数据分析进展。我会用这些将微生物组整合到神经发育的暴露组分析中的培训和研究活动。我将利用我接受的研究和职业发展培训来使自己成为一个独立的人具有终身教职的研究员，能够获得 R01 资金。该研究提案结合了创新的暴露测量、新颖的数据分析方法、和高分辨率组学分析，以解决对时间和潜在机制理解方面的差距金属暴露以及肠道微生物组和代谢组与神经发育相关注意力措施。拟议的研究和培训计划为独立的研究和培训奠定了基础旨在通过以下方式阐明环境暴露与神经发育之间的联系的研究生涯人类微生物组。