HORMONE ASSAY CORE

激素检测核心

基本信息

批准号：
7720957
负责人：
INDRANI SINHA-HIKIM
金额：
$ 47.91万
依托单位：
CHARLES R. DREW UNIVERSITY OF MED & SCI
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7720957
关键词：
Androstenedione Antibodies Applications Grants Arts Binding Biological Assay C-Peptide C-reactive protein Celite Clinical Investigator Collaborations Computer Retrieval of Information on Scientific Projects Database Data Development Dialysis procedure Enrollment Equilibrium Estradiol Estrone Ethanol Family suidae Follicle Stimulating Hormone Funding Generations Globulins Gonadal Steroid Hormones Grant Hormones Immune Sera Immunoassay Inflammatory Institution Insulin Interleukin-6 Iodination reaction Laboratories Measurement Measures Methods Molecular Numbers Patients Peer Review Publications Publishing Radioimmunoassay Research Research Ethics Committees Research Personnel Resources Sampling Serum Serum Globulins Services Site Source Standards of Weights and Measures Stanolone Techniques Testosterone Tracer United States National Institutes of Health Universities Validation Woman dehydroepiandrosterone ethyl acetate innovation myostatin polypeptide C success

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Hormone Assay Core Lab was the first component of the CRC to be developed following funding. The objective of the Hormone Assay Core Lab is to expand the hormone assay capabilities at Charles Drew University by developing and making available hormone measurement methods not currently available to the investigators. The Hormone Assay Core Laboratory has had considerable success in refining existing assays to meet specific needs of investigators, and in developing new assays. Below is a list of hormone assays that are currently available: 1. Serum LH: A 2-site directed fluoroimmunometric assay (FIA). 2. Serum FSH: A 2-site directed fluoroimmunometric assay (FIA). 3. Testosterone: The assay for testosterone uses an iodinated tracer and extraction of serum samples using anexane: ethyl acetate (2:3) mixture, prior to immunoassay. The laboratory has published the development of a highly sensitive testosterone assay that has been optimized for the measurement of low testosterone levels in women. 4. Free Testosterone: Free testosterone levels are measured by a direct equilibrium dialysis procedure. The laboratory has published the development and validation of the sensitive assay for the measurement of low free testosterone levels in women, and the generation of normative data in menstruating women. 5. Dihydrotestosterone: Serum samples are extracted and chromatographed through a Celite column. DHT concentrations are measured in the eluate from the Celite fractions by RIA. 6. Sex-Hormone Building Globulin (SHBG): Serum SHBG binding capacity is measured by using tracer amounts of [3H-DHT] and serial dilutions of DHT standards. 7. Insulin: Insulin assay uses a standard from Wellcome equated to 1st 1RP GG/304, antiporcine antibody from ICN and porcine insulin from Lilly for iodination. 8. C-Peptide: C-Peptide assay uses antisera, standards and iodination material supplied through the Eli Lilly Company and an ethanol separation technique. Androstenedione, DHEA, Estrone, estradiol, myostatin, inflammatory markers, C-reactive protein and IL-6. 9. Since its initial funding, the HAC Lab has provided state-of-the-art service, and has been highly innovative in developing new assays. The HAC Lab has encouraged collaborations between molecular biologists and clinical investigators. The HAC Lab has had an outstanding record of accomplishments; it has supported a large number of high-impact, peer-reviewed publications, and new RO1 grant applications. Note: The Core does not conduct any studies of it''s own. It provides hormone measurements to studies that are CRC and IRB approved. Therefore, it uses the same IRB approval as the umbrella CRC grant does. There is no patient enrollment by the Core. It is purely a hormone measurement laboratory.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。激素测定核心实验室是资助后要开发的CRC的第一个组成部分。激素测定核心实验室的目的是通过开发和提供目前尚不适合研究人员可用的激素测量方法来扩展查尔斯·德鲁大学的激素测定能力。激素测定核心实验室在完善现有测定方面取得了很大的成功，以满足调查人员的特定需求以及开发新测定法。以下是当前可用的激素测定的列表： 1。SerumLH：2个位置的氟免疫测定法（FIA）。 2。血清FSH：2个位置的氟免疫测定法（FIA）。 3.睾丸激素：睾丸激素的测定在免疫测定之前使用苯甲烷：乙酸乙酯（2：3）混合物使用碘化示踪剂和血清样品提取。该实验室已经发表了一种高度敏感的睾丸激素测定法，该测定法已优化了针对女性低睾丸激素水平的测量。 4。游离睾丸激素：免费睾丸激素水平通过直接平衡透析程序来衡量。该实验室已经发布了敏感测定法的开发和验证，以测量女性的自由睾丸激素水平低，以及月经妇女的规范性数据的产生。 5。二氢睾丸激素：通过celite柱提取血清样品并色谱。 DHT浓度是通过RIA从硅藻土级分中的洗脱液中测量的。 6。性激素构建球蛋白（SHBG）：通过使用[3H-DHT]的示踪剂量和DHT标准的串行稀释液来测量血清SHBG结合能力。 7。胰岛素：胰岛素测定法使用等于1st 1rp gg/304的惠康的标准，来自lilly的ICN和猪胰岛素的抗逆强抗体进行碘化。 8。C肽：C肽测定法使用Eli Lilly Company和乙醇分离技术提供的抗血清，标准和碘化材料。雄激素，DHEA，雌酮，雌二醇，肌抑制素，炎症标记，C反应蛋白和IL-6。 9.自首次资金以来，HAC实验室提供了最先进的服务，并且在开发新测定方面具有高度创新性。 HAC实验室鼓励分子生物学家与临床研究人员之间的合作。 HAC实验室的成就记录出色。它支持大量的高影响力，同行评审的出版物和新的RO1赠款应用程序。注意：核心不会对其进行任何研究。它为CRC和IRB批准的研究提供了激素测量。因此，它使用了与雨伞CRC赠款相同的IRB批准。核心没有患者入学。它纯粹是一种激素测量实验室。