BROWN UNIVERSITY CONSORTIUM - PHENOTYPING CORE

布朗大学联盟 - 表型核心

• 批准号: 7610572
• 负责人: ELAINE L BEARER
• 金额: $ 13.72万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610572
• 关键词: Cell Transplantation; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Confocal Microscopy; DNA; Funding; Geographic Locations; Grant; Green Fluorescent Proteins; Image; Immunohistochemistry; Institution; Lasers; Marrow; Microscope; Microscopy; Morphology; Motor; Nature; Phenotype; Research; Research Personnel; Resources; Scanning; Skeletal Muscle; Skin; Source; Standards of Weights and Measures; Suspension substance; Suspensions; Tissues; United States National Institutes of Health; Universities; beta-Galactosidase; cell type; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core focuses on identifying the nature of cells in skin and skeletal muscle after marrow cells transplantation. The overall plan has been for donor cells and their specific lineages to be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. In this plan, donor cells can be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of make DNA. The lineage of identified donor cells can be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers canbe confirmed using confocal or deconvolution fluorescent microscopy. The core canprovide high-level expertise for these approaches. The core contains two Zeiss fluorescent motor-driven microscopes, an upright Axioplan 2 and an inverted Axiovert 200M, and a Zeiss 510 confocal laser-scanning microscope capable of four-color imaging. The core is capable of standard deconvolution fluorescent microscopy, 3D reconstructions and confocal microscopy. These latter, plus photomapping can be used to simulateously show a donor marker and a cell type specific marker.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该核心重点是识别骨髓细胞移植后皮肤和骨骼肌中细胞的性质。总体计划是供体细胞及其特定谱系，以通过共焦和反卷积荧光显微镜确定和共定位。在此计划中，可以通过表达GFP，β-半乳糖苷酶或Make DNA的存在在组织切片或细胞悬浮液中跟踪供体细胞。可以通过标准形态，地理位置和免疫组织化学来确定已确定的供体细胞的谱系。使用共聚焦或反向荧光显微镜确认供体和谱系标记的共定位。核心可以为这些方法提供高级专业知识。核心包含两个Zeiss荧光运动驱动的显微镜，一个直立的Axioplan 2和一个倒置的200m，以及一个能够具有四色成像的Zeiss 510共聚焦激光扫描显微镜。核心能够进行标准的反卷积荧光显微镜，3D重建和共聚焦显微镜。这些后者，加上光映用品可用于模拟显示供体标记和细胞类型特异性标记。