Multispectral and Hyperspectral Preclinical Imager Spanning the Visible, NIR-I and NIR-II

跨越可见光、NIR-I 和 NIR-II 的多光谱和高光谱临床前成像仪

• 批准号: 10193523
• 负责人: David A Boas
• 金额: $ 40.43万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10193523
• 关键词: Arts; Bioluminescence; Boston; Cells; Complex; Contrast Media; Custom; Development; Disease; Engineering; Equipment; Funding; Future; Health; Human; Image; Light; Microscopy; Mus; Optics; Penetration; Recovery; Research; Research Personnel; Resources; Rivers; Science; System; Techniques; Tissues; United States National Institutes of Health; Universities; base; bioluminescence imaging; cancer imaging; college; cost; design; fluorescence imaging; imager; imaging capabilities; improved; in vivo imaging system; instrument; interest; light scattering; nanomedicine; nanoparticle; optical imaging; pre-clinical; programs; whole animal imaging

Project Summary/Abstract This is a proposal from ten investigators from the College of Engineering, the College of Arts and Sciences and Sargent College at Boston University (BU) requesting purchase of a preclinical imager for photoluminescence and bioluminescence in mice. While the researchers at Boston University make extensive use of light-based optical imaging, primarily with advanced microscopy techniques on campus as well as off-campus in vivo imaging systems, there currently is no whole animal imaging capability on the BU Charles River Campus. Acquisition of a preclinical imager will allow the researchers to advance their NIH funded research programs that depend on the examination of complex, intact mammalian systems. The users propose the purchase of a first-to-market imager that will enable both traditional fluorescence and bioluminescence imaging in the visible and first near infrared (NIR-I) optical tissue window wavelength ranges (500 – 650 nm and 650 – 900 nm, respectively) as well as shortwave infrared (SWIR, also known as the second NIR optical tissue window or NIR-II) imaging. By using light in the 1000 – 1700 nm range, deeper penetration depths are achieved with less light scattering, leading to unprecedented clarity in tissue-depth light-based imaging. By designing a customized instrument to cover this extended wavelength range, the BU researchers will be able to continue current research directions that depend on traditional imaging more quickly and conveniently using local, on-campus equipment, while also hastening the funded development of SWIR contrast agents and enabling future directions using SWIR imaging that are beyond what is openly available to any researcher in the region. The users for the proposed instrument have a diversity of research interests. The proposed applications of the preclinical imager are typically focused on the tracking of engineered cells using bioluminescence, the imaging of tumors and vasculature with exogenous contrast agents, and the tracking of nanoparticles for nanomedicine applications. In this proposal, we describe the instrument we aim to acquire, the benefits of this particular instrument over others, provide a management plan for instrument use and cost recovery, and illustrate the institutional commitment to maintaining this resource. Overall, the acquisition of this preclinical imager will support projects focused on improving diverse aspects of human health and the treatment of disease.

项目概要/摘要 这是来自工程学院、艺术与科学学院和 波士顿大学 (BU) 萨金特学院请求购买用于光致发光的临床前成像仪 和小鼠的生物发光，而波士顿大学的研究人员广泛使用基于光的技术。 光学成像，主要采用校园内先进的显微镜技术以及校外的体内成像 系统，目前 BU 查尔斯河校区没有整体动物成像能力。 临床前成像仪将使研究人员能够推进他们的 NIH 资助的研究项目，这些项目依赖于 对复杂、完整的哺乳动物系统的检查 用户建议购买第一个上市的系统。 成像仪将在可见光和第一近光区域实现传统的荧光和生物发光成像 红外 (NIR-I) 光学组织窗口波长范围（分别为 500 – 650 nm 和 650 – 900 nm） 作为短波红外（SWIR，也称为第二近红外光学组织窗口或NIR-II）成像。 1000 – 1700 nm 范围内的光，可以通过较少的光散射实现更深的穿透深度，从而 通过设计定制仪器来实现基于光的组织深度成像前所未有的清晰度。 扩展的波长范围，BU研究人员将能够继续当前的研究方向 使用本地校园设备比传统成像更快速、更方便，同时也加快了 资助开发短波红外造影剂，并利用短波红外成像实现未来方向 超出了该地区任何研究人员可以公开获得的内容。 所提出的仪器的用户有不同的研究兴趣。 临床前成像仪通常专注于使用生物发光来跟踪工程细胞，成像 用外源性造影剂观察肿瘤和脉管系统，以及纳米医学中纳米粒子的追踪 应用程序。 在本提案中，我们描述了我们旨在获得的工具，以及该特定工具相对于 其他，提供仪器使用和成本回收的管理计划，并说明制度 总体而言，购买该临床前成像仪将支持项目。 专注于改善人类健康和疾病治疗的各个方面。