Targeting Smooth Muscle Progenitor Cells for Treatment of Pulmonary Arterial Hypertension

靶向平滑肌祖细胞治疗肺动脉高压

基本信息

批准号：
10189689
负责人：
YOU-YANG ZHAO
金额：
$ 49.73万
依托单位：
LURIE CHILDREN'S HOSPITAL OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-06-01 至 2022-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10189689
关键词：
Address Angiogenic Factor Animal Model Attenuated Blood Pressure Blood Vessels CXCL12 gene CXCR4 gene Cell Cycle Cells Cessation of life Chronic Clinical Data Diphtheria Toxin Disease Distal Endothelial Cells Endothelium FOXM1 gene Genetic Heart Hypertrophy Heart failure Hypertension Hypoxia Hypoxia Inducible Factor Lead Lesion Lung Lung diseases Mediating Modeling Molecular Monocrotaline Mus Muscle Muscle satellite cell Pathogenesis Pathologic Patients Pharmacology Play Population Procollagen-Proline Dioxygenase Pulmonary Vascular Resistance Rattus Reporter Reporting Resistance Role Smooth Muscle Smooth Muscle Actin Staining Method Smooth Muscle Myocytes Structure of parenchyma of lung Tamoxifen Testing Therapeutic Thiostrepton Tissues Vascular remodeling adenoviral-mediated alpha Actin arterial lesion arteriole diphtheria toxin receptor druggable target effective therapy forkhead protein inhibitor/antagonist intima media mortality mouse model new therapeutic target novel novel therapeutic intervention premature primary pulmonary hypertension progenitor prominin promoter pulmonary arterial hypertension receptor expression recombinant adenovirus stem cells

项目摘要

Pulmonary arterial hypertension (PAH) is characterized by obliterative pulmonary vascular remodeling and progressive elevation of pulmonary vascular resistance that leads to right heart failure and premature death. Although great efforts have been made to treat PAH, current therapies fail to reverse the disease and mortality remains high. Comprehensive understanding of the mechanisms underlying obliterative pulmonary vascular remodeling is warranted to identify druggable targets for effective treatment of PAH. Accumulation of smooth muscle cell (SMC) in the pulmonary vascular lesions is the hallmark of obliterative pulmonary vascular remodeling. We have recently identified the first mouse model of PAH [Tie2Cre-mediated disruption of Egln1, encoding hypoxia inducible factor (HIF) prolyl hydroxylase 2 (PHD2), designated Egln1Tie2Cre] with progressive obliterative vascular remodeling including vascular occlusion and plexiform-like lesion, and right heart failure, which recapitulates many features of clinical PAH. Using this mouse model as well as the Sugen/Hypoxia rat model, we identified a subpopulation of smooth muscle progenitor cells expressing CD133 (a marker of progenitor cells) (CD133+ SMPCs) which were enriched at the occlusive vascular lesions as well as the plexiform-like lesions and muscularized pulmonary arterioles. These cells expressed high levels of the cell cycle master regulator Forkhead Box M1 (FoxM1), indicating the highly proliferative potential. Genetic depletion of CD133+ cell population inhibited chronic hypoxia-induced PH. We also observed decreased vascular remodeling and PH in mice with tamoxifen-inducible deletion of Foxm1 in smooth muscle cells. Pharmacological inhibition of FoxM1 attenuated PAH in Sugen/Hypoxia-exposed rats. Thus, we hypothesize that EC-SMPC crosstalk regulates CD133+ SMPC proliferation in a FoxM1-dependent manner and thereby plays a fundamental role in the mechanisms of obliterative vascular remodeling and severe PAH. The proposed studies will address the following Specific Aims. In Aim 1, we will determine the role of smooth muscle progenitor cells in the mechanisms of pulmonary vascular remodeling and PAH. In Aim 2, we will delineate the molecular mechanisms of SMPC-mediated vascular remodeling in PAH. In Aim 3, we will explore the translational potential of targeting FoxM1 for treatment of PAH. We expect that the proposed studies have significant translational potential by elucidating the fundamental mechanisms of obliterative vascular remodeling and identifying druggable targets that can pharmacologically reverse obliterative vascular remodeling for the treatment of severe PAH in patients.

肺动脉高压（PAH）的特点是闭塞性肺血管重构和肺血管阻力逐渐升高，导致右心衰竭和过早死亡。尽管人们为治疗 PAH 付出了巨大的努力，但目前的治疗方法未能扭转疾病和死亡率仍然处于高位。全面了解闭塞性肺血管的机制需要进行重构来确定有效治疗 PAH 的药物靶点。积累平滑肺血管病变中的肌细胞（SMC）是闭塞性肺血管的标志重塑。我们最近发现了第一个 PAH 小鼠模型 [Tie2Cre 介导的 Egln1 破坏，编码缺氧诱导因子 (HIF) 脯氨酰羟化酶 2 (PHD2)，命名为 Egln1Tie2Cre] 闭塞性血管重塑，包括血管闭塞和丛状病变，以及右心衰竭，它概括了临床 PAH 的许多特征。使用该小鼠模型以及 Sugen/缺氧大鼠在模型中，我们鉴定了表达 CD133（CD133 的标记物）的平滑肌祖细胞亚群。祖细胞）（CD133+ SMPC）在闭塞血管病变处以及丛状病变和肌化肺小动脉。这些细胞表达高水平的细胞周期主调节器Forkhead Box M1（FoxM1），表明具有高度增殖潜力。基因耗竭 CD133+细胞群抑制慢性缺氧诱导的PH。我们还观察到血管重塑减少和他莫昔芬诱导平滑肌细胞中 Foxm1 缺失的小鼠的 PH。药理抑制 FoxM1 可减轻 Sugen/缺氧暴露大鼠中的 PAH。因此，我们假设 EC-SMPC 串扰以 FoxM1 依赖性方式调节 CD133+ SMPC 增殖，从而在闭塞性血管重塑和严重 PAH 的机制。拟议的研究将解决遵循具体目标。在目标 1 中，我们将确定平滑肌祖细胞在肺血管重构和PAH的机制。在目标 2 中，我们将描述分子机制 PAH 中 SMPC 介导的血管重塑。在目标 3 中，我们将探索靶向的转化潜力 FoxM1 用于治疗 PAH。我们预计所提出的研究具有重大的转化潜力阐明闭塞性血管重塑的基本机制并确定可药物靶点可以在药理学上逆转闭塞性血管重塑，以治疗严重 PAH 患者。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Dry Powder for Pulmonary Delivery: A Comprehensive Review.

用于肺部输送的干粉：综合评价。

DOI：
发表时间：
2020-12-28
期刊：
影响因子：
5.4
作者：
Chaurasiya, Birendra;Zhao, You
通讯作者：
Zhao, You

Endothelial FoxM1 reactivates aging-impaired endothelial regeneration for vascular repair and resolution of inflammatory lung injury.

内皮 FoxM1 重新激活老化受损的内皮再生，从而修复血管并解决炎症性肺损伤。

DOI：
发表时间：
2023-08-16
期刊：
影响因子：
17.1
作者：
Huang, Xiaojia;Zhang, Xianming;Machireddy, Narsa;Evans, Colin E;Trewartha, Shawn D;Hu, Guochang;Fang, Yun;Mutlu, Gökhan M;Wu, David;Zhao, You
通讯作者：
Zhao, You

YAP expression in endothelial cells prevents ventilator-induced lung injury.

内皮细胞中的 YAP 表达可预防呼吸机引起的肺损伤。

DOI：
发表时间：
2021
期刊：
影响因子：
0
作者：
Su, Kai;Wang, Jianguo;Lv, Yang;Tian, Ming;Zhao, You;Minshall, Richard D;Hu, Guochang
通讯作者：
Hu, Guochang

Suppression of BMP signaling by PHD2 deficiency in Pulmonary Arterial hypertension.

肺动脉高压中 PHD2 缺陷对 BMP 信号传导的抑制。

DOI：
发表时间：
2022-01
期刊：
影响因子：
2.6
作者：
Liu, Bin;Yi, Dan;Pan, Jiakai;Dai, Jingbo;Zhu, Maggie M;Zhao, You;Oh, S Paul;Fallon, Michael B;Dai, Zhiyu
通讯作者：
Dai, Zhiyu

Loss of Endothelial Hypoxia Inducible Factor-Prolyl Hydroxylase 2 Induces Cardiac Hypertrophy and Fibrosis.

内皮缺氧诱导因子脯氨酰羟化酶 2 的丧失会导致心脏肥大和纤维化。

DOI：
发表时间：
2021
期刊：
影响因子：
5.4
作者：
Dai, Zhiyu;Cheng, Jianding;Liu, Bin;Yi, Dan;Feng, Anlin;Wang, Ting;An, Lingling;Gao, Chen;Wang, Yibin;Zhu, Maggie M;Zhang, Xianming;Zhao, You
通讯作者：
Zhao, You