CORE--CELLULAR NEUROSCIENCE CORE

核心--细胞神经科学核心

• 批准号: 7670394
• 负责人: David E. Pleasure
• 金额: $ 25.53万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7670394
• 关键词: Animal Model; Animals; Arts; Biochemistry; Biology; Brain; Cell Culture Techniques; Cell physiology; Cells; Childhood; Childhood Brain Neoplasm; Color; Complement; Condition; Confocal Microscopy; Consultations; Counseling; Cultured Cells; Data; Depth; Development; Developmental Disabilities; Disease; Doctor of Medicine; Doctor of Philosophy; Electrophysiology (science); Embryo; Ensure; Epilepsy; Equipment; Evolution; Functional disorder; Funding; Generations; Genes; Genetic; Genotype; Goals; Growth; Head; Histopathology; Human; Human Resources; Image; Imagery; In Situ Hybridization; Institution; Interdisciplinary Study; Investments; Lasers; Leadership; Life; Mental Retardation; Mental Retardation and Developmental Disabilities Research Centers; Methods; Microscope; Microscopy; Mission; Mitochondria; Modeling; Molecular; Molecular Genetics; Mutagenesis; Natural regeneration; Neuraxis; Neurodegenerative Disorders; Neurologist; Neurons; Neurosciences; Pathogenesis; Pathology; Pediatric Brain Injury; Pediatric Hospitals; Peripheral; Phenotype; Physiological; Policies; Productivity; Proteins; Publications; Purpose; Reagent; Research Institute; Research Personnel; Services; Specimen; Spectrum Analysis; Staging; Surveys; System; Techniques; Technology; Tissues; Transgenic Organisms; Work; base; experience; gene discovery; innovation; instrument; instrumentation; interest; member; micromanipulator; neurochemistry; neuropathology; new technology; novel; optical imaging; pleasure; response; satisfaction; skills; tissue preparation; tumor

The initial (1999) application did not include a Cellular Neuroscience Core. Instead, it proposed separate cores in Neuropathology and Neuroscience, with the latter offering expertise in cell culture. The Director of the Neuroscience Core was David Pleasure, M.D., an internationally recognized neurologist with interests in glial biology of the peripheral and central nervous system. Neuropathology initially was headed by John Trojanowski, M.D., Ph.D. and Dr. Lucy Rorke, M.D., distinguished neuropathologists with respective eminence in neurodegenerative diseases and pediatric brain tumors. As user needs evolved, it became apparent that these facilities should be merged into a single Cellular Neuroscience Core. This was accomplished in 1994 with Dr. Pleasure as the Director. He served in this capacity through the renewal of the Center (1999) until 2001, when leadership passed to Dr. Jeffrey Golden, a pediatric neuropathologist with a primary interest in brain development and its disorders. Dr. Golden first joined CHOP in 1997 and immediately became engaged with the MRDDRC, both as a user and as an Associate Director of the Cellular Neuroscience Core. Dr. Pleasure has continued to serve as the Director of the Stokes Research Institute at CHOP. Since its inception (1994) our Core has provided users with a diverse repertoire of state-of-the-art methods for visualization of the distributions of gene products in normal, developing neural cells and in those undergoing various forms of degeneration and regeneration. We have continually and eagerly added new skills, instrumentation and reagents to better serve our users's needs. In 1995, with the generous assistance of the Children's Hospital, we purchased a Leica confocal microscope to which we added inverted microscopy, stagemounted micromanipulators/microinjectors, and a stage-mounted environmental chamber, thus permitting prolonged observation and manipulation of living cells under physiological conditions. In this manner we offered 8-color capacity fluorescent imaging. Our institution paid for the apparatus and we used MRDDRC funding to partially support a technician who worked with Dr. Peter Bannerman, the Director of Confocal Microscopy. Dr. Bannerman became very skillful in the use of this instrument and he made this valuable expertise available to MRDDRC researchers. We made several other notable technological additions to the Core repertoire, including in situ hybridization in both sections and whole embryos. Our general purpose has been not only to make available a technology, but a consultative service that facilitates implementation of the method as well as the interpretation of data. We adhered to this policy when we also added video-enhanced microscopy in order to better support MRDDRC investigators in analyzing intracellular Ca2+ and Na+ as well as the estimate of mitochondrial potential. We were especially gratified that Dr. Douglas Coulter joined the Core upon his coming to CHOP in 1999. Dr. Coulter added to our "armamentarium" his deep experience in electrophysiology and his background in studying the epilepsies. We also welcomed (2003) Dr. Alex Judkins, a pediatric neuropathologist with interests in pediatric brain injury and pediatric brain tumors v atypical teratoid/rhabadoid tumors and primative neuroectodermal tumors in particular. Supported by funds from the Department of Pathology and CHOP, Dr. Judkins oversees the newly equipped histopathology labs tissue microarrayer and laser-capture microscopy systems. These systems, which are now being utilized by center investigators, will also complement services provided by the Molecular Genetics core and the Analytical Neurochemistry cores. Advances in genetics have rapidly accelerated the discovery of genes implicated in the pathogenesis of the developmental disabilities, including several genes that have been identified by members of our MRDDRC. To better appreciate genotype-phenotype relationships, the Cellular Neuroscience Core plans to offer transgenic and mutagenesis services during the next funding period. The goal is to generate animal models for the specific intent to study disorders relevant to the MRDDRC mission. Dr. Golden has overseen the establishment of a core at CHOP to perform these services. The MRDDRC plans to provide consultation and economical access to this new facility. This exciting advance will help investigators to scrutinize the basis of disease in hitherto inaccessible detail. Indeed, we note with satisfaction that MRDDRC users will explore these models not only with the techniques that our Core offers, but with the plethora of methods that are available through the Molecular Genetics and Analytical Neurochemistry and Spectroscopy Cores. As a result of the continued evolution of this core and the investment by the institution in new technologies and the constant upgrading of equipment, the Cellular Neuroscience Core has been able to provide outstanding services to Center users. Over the past 4.5 years we have serviced 25 investigators; contributing to at least 49 publications. The growth of existing services and the addition of new methods ensures that MRDDRC members enjoy ready access to that technological array that has become so essential to all contemporary interdisciplinary research.

最初（1999 年）的应用程序不包括细胞神经科学核心。相反，它提出了单独的核心 神经病理学和神经科学，后者提供细胞培养方面的专业知识。董事 神经科学核心是 David Pleasure 医学博士，一位国际公认的神经学家，对神经胶质细胞感兴趣 周围和中枢神经系统的生物学。神经病理学最初由约翰领导 特洛伊诺夫斯基，医学博士，博士和 Lucy Rorke 博士，医学博士，分别是杰出的神经病理学家 神经退行性疾病和儿童脑肿瘤。随着用户需求的发展，这些内容变得显而易见 设施应合并为一个细胞神经科学核心。这是由 Dr. 于 1994 年完成的。 作为导演很高兴。他在中心更新期间（1999 年）一直担任这一职务，直到 2001 年 领导权移交给杰弗里·戈尔登 (Jeffrey Golden) 博士，他是一位主要对大脑感兴趣的儿科神经病理学家 发育及其障碍。 Golden 博士于 1997 年首次加入 CHOP，并立即与 MRDDRC，作为细胞神经科学核心的用户和副主任。快乐博士有 继续担任 CHOP 斯托克斯研究所所长。 自成立（1994 年）以来，我们的 Core 已为用户提供了多种最先进的方法，用于 正常、发育中的神经细胞和正在经历的神经细胞中基因产物分布的可视化 各种形式的退化和再生。我们不断地热切地增加新技能， 仪器和试剂，以更好地满足用户的需求。 1995年，在省政府的大力支持下 在儿童医院，我们购买了一台徕卡共焦显微镜，并在其中添加了安装在舞台上的倒置显微镜 显微操作器/显微注射器，以及安装在舞台上的环境室，从而允许 在生理条件下长时间观察和操作活细胞。通过这种方式我们 提供 8 色容量荧光成像。我们的机构支付了设备费用并使用了 MRDDRC 资助部分支持与 Confocal 总监 Peter Bannerman 博士合作的技术人员 显微镜检查。 Bannerman 博士非常熟练地使用该仪器，并且他使该仪器变得有价值 MRDDRC 研究人员可以获得的专业知识。 我们对核心库做了其他一些值得注意的技术补充，包括原位杂交 切片和整个胚胎。我们的总体目标不仅是提供一种技术，而且是一种 促进方法实施以及数据解释的咨询服务。我们 为了更好地支持 MRDDRC，我们还添加了视频增强显微镜，并遵守了这一政策 研究人员分析细胞内 Ca2+ 和 Na+ 以及线粒体电位的估计。 我们特别高兴的是，Douglas Coulter 博士于 1999 年来到 CHOP 后加入了核心。 库尔特将他在电生理学方面的丰富经验和他的背景添加到我们的“军备库”中 研究癫痫病。我们还迎来了 (2003) Alex Judkins 博士，他是一位感兴趣的儿科神经病理学家 小儿脑损伤和小儿脑肿瘤 v 非典型畸胎瘤样/横纹肌样瘤和原发性肿瘤 尤其是神经外胚层肿瘤。在病理学系和 CHOP 的资金支持下，Dr. 贾金斯负责监督新配备的组织病理学实验室组织微点阵仪和激光捕获显微镜 系统。这些系统目前正被中心调查员使用，也将补充服务 由分子遗传学核心和分析神经化学核心提供。 遗传学的进步迅速加速了与疾病发病机制有关的基因的发现。 发育障碍，包括我们的 MRDDRC 成员已确定的几个基因。到 为了更好地理解基因型与表型的关系，细胞神经科学核心计划提供转基因 以及下一个资助期间的诱变服务。目标是为特定的动物模型 旨在研究与 MRDDRC 任务相关的疾病。 Golden 博士监督建立了一个核心 在 CHOP 执行这些服务。 MRDDRC 计划为此提供咨询和经济途径 新设施。这一令人兴奋的进展将有助于研究人员仔细研究迄今为止疾病的基础 难以接近的细节。事实上，我们满意地注意到 MRDDRC 用户不仅会探索这些模型 使用我们的核心提供的技术，但通过可通过 分子遗传学和分析神经化学和光谱学核心。 由于该核心的不断发展以及机构对新技术和新产品的投资 随着设备的不断升级，细胞神经科学核心已经能够提供出色的 为中心用户提供服务。在过去 4.5 年里，我们为 25 名调查员提供了服务；至少贡献了 49 出版物。现有服务的增长和新方法的添加确保了 MRDDRC 成员 随时获取对所有当代跨学科都至关重要的技术组合 研究。