MOLECULAR LOGIC OF SUBSTRATE RECOGNITION BY THE CULLIN-4 UBIQUITIN LIGASE

CULLIN-4 泛素连接酶识别底物的分子逻辑

• 批准号: 7602224
• 负责人: NING ZHENG
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602224
• 关键词: Affinity Chromatography; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Cullin Proteins; Family; Funding; Grant; Human; Institution; Logic; Mass Spectrum Analysis; Molecular; Proteins; Recruitment Activity; Research; Research Personnel; Resources; Source; Ubiquitination; United States National Institutes of Health; base; receptor; ubiquitin ligase; ubiquitin-protein ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cullin based ubiquitin ligases are the largest family of E3 ligases. There is 7 Cullins in humans and every Cullin utilizes a different family of substrate specific receptors in order to recruit the substrate to the ubiquitination machinery. Although the family of substrate specific receptors are known for most of the Cullins, the identity of this family for the Cullin-4 family remains elusive. Using tandem-affinity purification of Cullin-4 and DDB1 protein complexes from human cells and mass spectrometry we will attempt to 1) identify and 2) characterize the family of substrate-specirfic receptors for Cullin-4.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 基于 Cullin 的泛素连接酶是最大的 E3 连接酶家族。 人类中有 7 个 Cullins，每个 Cullins 都利用一个 不同家族的底物特异性受体，以将底物招募到泛素化机制中。 虽然 大多数 Cullins 都知道底物特异性受体家族，该家族的身份为 Cullin-4 家族 仍然难以捉摸。 使用串联亲和纯化来自人体细胞和团块的 Cullin-4 和 DDB1 蛋白复合物 通过光谱分析，我们将尝试 1) 识别和 2) 表征 Cullin-4 底物特异性受体家族。