CYCLIN C-ASSOCIATED PROTEINS

细胞周期蛋白 C 相关蛋白

• 批准号: 7602117
• 负责人: RANDY S STRICH
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602117
• 关键词: 26S proteasome; Binding; Computer Retrieval of Information on Scientific Projects Database; Cyclin-Dependent Kinases; Cyclins; Funding; Genes; Grant; Holoenzymes; Institution; Meiosis; Oxidative Stress; Proteins; RNA Polymerase II; Repression; Research; Research Personnel; Resources; Signal Transduction; Source; Ubiquitin; United States National Institutes of Health; Yeasts; biological adaptation to stress; cyclin C

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal focuses on the yeast C-type cyclin and the cyclin dependent kinase Cdk8. Cyclin C-Cdk8p binds the RNA polymerase II holoenzyme and represses both meiotic and stress response genes. To relieve this repression, cyclin C is destroyed thus uncovering a unique set of regulatory signals controlling cyclin turnover. We have found that the oxidative-stress induced destruction of cyclin C requires ubiquitin and the 26S proteasome. The focus of this study is to identify proteins that associate with cyclin C.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该提案重点关注酵母 C 型细胞周期蛋白和细胞周期蛋白依赖性激酶 Cdk8。 细胞周期蛋白 C-Cdk8p 结合 RNA 聚合酶 II 全酶并抑制减数分裂和应激反应基因。 为了缓解这种抑制，细胞周期蛋白 C 被破坏，从而揭示了一组控制细胞周期蛋白周转的独特调节信号。我们发现氧化应激诱导的细胞周期蛋白 C 破坏需要泛素和 26S 蛋白酶体。 本研究的重点是鉴定与细胞周期蛋白 C 相关的蛋白质。