CHAPERONIN (SULFOLOBUS SHIBATAE)

伴侣蛋白（柴田硫化叶）

• 批准号: 7598624
• 负责人: JONATHAN C TRENT
• 金额: $ 0.81万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598624
• 关键词: Archaea; Cells; Computer Retrieval of Information on Scientific Projects Database; Condition; Cryoelectron Microscopy; Funding; Goals; Grant; Growth; Heat-Shock Response; Higher Order Chromatin Structure; Institution; Lipids; Liposomes; Location; Nanotechnology; Proteins; Research; Research Personnel; Resources; Source; Structure; Sulfolobus; United States National Institutes of Health; chaperonin; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will focus on group II chaperonins from the hyperthermophilic archaeon, Sulfolobus shibatae. The chaperonins in S. shibatae are composed of some of the most abundant proteins in the cells under all growth conditions. The goals of this project are to use cryo-electron microscopy to: 1)investigate if the chaperonin double rings, called "rosettasomes", are the functional form in vivo or if some higher-order structures are present; 2) characterize the higher-order structures of chaperonins to elucidate how they may function in vivo and investigate how they can be used in nanotechnology; and 3) visualize the chaperonin structures in cells before and after heat shock. In addition to investigating the structure and location of chaperonins in vivo, we will investigate chaperonin structures in association with liposomes and lipid layers. This will support our search for these chaperonins in vivo and will provide important structural information about higher-order structures of chaperonins that we have been using for applications in nanotechnology.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目将重点关注来自硫磺sulfolobus shibatae的高素古学的II组伴侣蛋白。 S. shibatae中的伴侣蛋白由在所有生长条件下细胞中一些最丰富的蛋白质组成。 该项目的目标是使用冷冻电子显微镜进行： 1）研究伴侣蛋白双环（称为“ rosettasomes”）是体内的功能形式，还是存在一些高阶结构； 2）表征伴侣蛋白的高阶结构，以阐明它们在体内的作用，并研究如何在纳米技术中使用它们； 3）在热休克之前和之后可视化细胞中的伴侣蛋白结构。 除了研究体内伴侣蛋白的结构和位置外，我们还将研究与脂质体和脂质层相关的伴侣蛋白结构。这将支持我们在体内对这些伴侣蛋白的搜索，并将提供有关我们一直在纳米技术中应用的伴侣蛋白高阶结构的重要结构信息。