FLUORESCENCE LIFETIMES OF NADH IN GLYCEROL/WATER AND TREHALOSE

甘油/水和海藻糖中 NADH 的荧光寿命

• 批准号: 7598458
• 负责人: Jane Marie Vanderkooi
• 金额: $ 0.16万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598458
• 关键词: Cells; Computer Retrieval of Information on Scientific Projects Database; Dependence; Fluorescence; Funding; Glass; Glycerol; Grant; Image; Institution; Location; Oxidation-Reduction; Range; Relaxation; Research; Research Personnel; Resources; Source; Sucrose; Temperature; Tissues; Trehalose; United States National Institutes of Health; Water; cold temperature; fluorescence imaging; sugar; water solution; Cells; Computer Retrieval of Information on Scientific Projects Database; Dependence; Fluorescence; Funding; Glass; Glycerol; Grant; Image; Institution; Location; Oxidation-Reduction; Range; Relaxation; Research; Research Personnel; Resources; Source; Sucrose; Temperature; Tissues; Trehalose; United States National Institutes of Health; Water; cold temperature; fluorescence imaging; sugar; water solution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fluorescence imaging of cells and tissue can be used to evaluate beta-NADH redox and location. At low temperature beta-NADH fluorescence intensity is known to increase and therefore sensitivity of imaging increases. We have evaluated the temperature dependence of steady-state fluorescence for beta-NADH in glycerol/water solution and in trehalose/sucrose glass. The temperature range studied was 290 K to 12 K. The steady-state fluorescence of beta-NADH in glycerol/water was found to increase ~16 fold and the emission to shift by about 35 nm to the blue as temperature decreased. Much smaller changes were seen for fluorescence of beta-NADH in sugar glass. It is suggested that the sensitivity of beta-NADH fluorescence is related to water relaxation around the excited state molecule. We would like to probe further if the fluorescence lifetime of beta-NADH is dependent upon water concentration in glycerol/water mixtures.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 细胞和组织的荧光成像可用于评估β-NADH氧化还原和位置。在低温下，β-NADH荧光强度会增加，因此成像的敏感性增加。我们已经评估了甘油/水溶液和海藻糖/蔗糖玻璃中β-NADH稳态荧光的温度依赖性。研究的温度范围为290 k至12K。发现β-nadh在甘油/水中的稳态荧光增加了约16倍，并且随着温度降低，排放的发射向蓝色约35 nm。在糖玻璃中，β-nadh的荧光观察到了较小的变化。建议β-NADH荧光的敏感性与激发态分子周围的水松弛有关。我们想进一步探究β-NADH的荧光寿命取决于甘油/水混合物中的水浓度。