CRYSTALLOGRAPHIC STUDIES OF MOLECULAR CHAPERONES AND RIBOZYMES

分子伴侣和核酶的晶体学研究

• 批准号: 7597904
• 负责人: David B McKay
• 金额: $ 0.28万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7597904
• 关键词: Bacteria; Catalytic RNA; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Family; Funding; Grant; Heat shock proteins; Homologous Gene; Institution; Membrane Proteins; Molecular Chaperones; Proteins; RNA; Research; Research Personnel; Resources; Source; Stress; Structure; System; United States National Institutes of Health; helicase; multicatalytic endopeptidase complex; stress protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are continuing our studies on the structures and mechanisms of molecular chaperone proteins which, broadly speaking, facilitate the correct folding, assembly and targeting of proteins and RNA molecules in cells. Systems that are currently under study include a "prokaryotic proteasome"--a 850 kDa complex that is a simple homolog of the eukaryotic proteasome; a family of "stress endurance" factors called universal stress proteins; a chaperone that facilitates folding and assembly of membrane proteins in bacteria; and a family of RNA chaperones/helicases that modulate RNA structure in cells. Additionally, we are continuing our studies on the structure and mechanism of small catalytic RNAs, or ribozymes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们正在继续研究分子伴侣蛋白的结构和机制，从广义上讲，分子伴侣蛋白有助于细胞中蛋白质和 RNA 分子的正确折叠、组装和靶向。 目前正在研究的系统包括“原核蛋白酶体”——一种 850 kDa 的复合物，是真核蛋白酶体的简单同系物；称为通用应激蛋白的“应激耐受”因子家族；促进细菌中膜蛋白折叠和组装的伴侣；以及调节细胞中 RNA 结构的 RNA 伴侣/解旋酶家族。 此外，我们正在继续研究小催化 RNA 或核酶的结构和机制。