SMALL ANGLE X-RAY SCATTERING STUDIES ON ACTIN BUNDLES

肌动蛋白束的小角 X 射线散射研究

• 批准号: 7598206
• 负责人: PAUL T. MATSUDAIRA
• 金额: $ 0.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598206
• 关键词: Actins; C-terminal; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Institution; Measurement; Measures; Positioning Attribute; Range; Relative (related person); Research; Research Personnel; Resources; Solutions; Source; United States National Institutes of Health; detector; radius bone structure; scruin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. From the spread or variability in the radius of gyration of scruin, we will measure the range in distances that separate the N-and C-terminal beta-propeller domains from the spread in the radius of gyration. Because scruin controls actin twist in the acrosomal bundle, the relative positions of the domains will give an upper boundry on the strain that can be stored in the twist of actin. We have previously collected solution scattering data to measure the Rg as a function of pCa. However, we cannot extract the variability in the Rg to the accuracy that we need. With improvements to the detector, we can re-collect the data and extract the measurements.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 根据SCRUIN回旋半径的扩散或变异性，我们将在距离内测量n和c末端β-螺旋桨域与回旋半径中的扩散区分开的范围。由于Scruin控制着蛇形束中的肌动蛋白扭曲，因此该结构域的相对位置将在菌株上产生上限，该菌株可以存储在肌动蛋白的扭曲中。我们先前已经收集了解决方案散射数据，以测量RG作为PCA的函数。但是，我们无法将RG中的可变性提取到所需的准确性。通过改进检测器，我们可以重新收集数据并提取测量结果。