CRYSTALLOGRAPHIC ANALYSIS OF THE ARABIDOPSIS SABATH FAMILY OF METHYLTRANSFERASES

拟南芥 SABATH 甲基转移酶家族的晶体分析

• 批准号: 7598111
• 负责人: JEANNINE R ROSS
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598111
• 关键词: Acids; Active Sites; Alkaloids; Anabolism; Arabidopsis; Auxins; Benzoic Acid; Benzoic Acids; Caffeine; Carboxylic Acids; Clarkia; Computer Retrieval of Information on Scientific Projects Database; Development; Enzymes; Esters; Family; Family member; Funding; Goals; Grant; Human; Hydrocarbons; Hydroxyl Radical; Institution; Length; Methyltransferase; Modeling; Morphine; Mouse-ear Cress; Nitrogen; Plant Components; Plant Growth Regulators; Plants; Play; Research; Research Personnel; Resolution; Resources; Role; Salicylic Acid; Salicylic Acids; Signal Transduction; Site-Directed Mutagenesis; Source; Structure; Tail; Theobromine; United States National Institutes of Health; base; disorder prevention; farnesoic acid; indoleacetic acid; isoprenoid; jasmonic acid; jasmonic acid carboxyl methyltransferase; nutrition; plant growth/development; salicylic acid carboxyl methyltransferase; small molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Small molecule methyltransferases (MTs) are ubiquitous throughout the plant kingdom and methylate the hydroxyl, carboxyl, or nitrogen moieties of small molecules to form products which perform critical functions in plant defense, human nutrition and disease prevention. Type III plant MTs, also known as the SABATH (for Salicylic Acid, Benzoic Acid, Theobromine synthase) family of MTs, convert small molecule carboxylic acids to their methyl esters, which have been shown to play vital roles in plant defense, signal transduction, floral scent, and development. This group also includes a subset of N-methyltransferases that target the nitrogen functionality of alkaloid compounds, a group which includes intermediates in caffeine and morphine biosynthesis. Our lab has made good progress in characterizing structures of plant MTs due to use of the SSRL facility: the first structure of a SABATH family member, Salicylic acid carboxyl methyltransferase (SAMT), an enzyme from Clarkia breweri with functions in floral scent and plant defense was solved to 3.0 ¿ resolution, and most recently, the first structure of an Arabidopsis thaliana SABATH family member, the Indole-3-acetic acid (auxin) carboxyl methyltransferase (IAMT) has been solved to 2.85 ¿ resolution. Active site modeling of the At Jasmonic acid carboxyl methyltransferase (JMT) based on the solved structure of the Clarkia SAMT allowed for the successful determination of critical JMT active site residues, and site-directed mutagenesis was used to create a bifunctional SAMT capable of using both salicylic acid and jasmonic acid as substrates. Active site modeling of the At Farnesoic acid methyltransferase (FAMT) has allowed for modulation of the acceptance of isoprenoid substrates with hydrocarbon tails of varying chain lengths. Our current goals are to structurally characterize the At FAMT, and another At SABATH family member which methylates gibberrellic acid, another plant hormone involved in all aspects of plant growth and development.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 小分子甲基转移酶 (MT) 在整个植物界中普遍存在，可将小分子的羟基、羧基或氮部分甲基化，形成在植物防御、人类营养和疾病预防中发挥关键功能的产品，也称为 III 型植物 MT。 SABATH（水杨酸、苯甲酸、可可碱合成酶）MT 家族，将小分子羧酸转化为其甲基酯类，已被证明在植物防御、信号转导、花香和发育中发挥着重要作用。该组还包括以生物碱化合物的氮功能为目标的 N-甲基转移酶子集，该类生物碱化合物包括咖啡因和咖啡因的中间体。由于使用 SSRL 设施，我们的实验室在表征植物 MT 结构方面取得了良好进展：SABATH 家族成员水杨酸羧基甲基转移酶的第一个结构。 (SAMT)，一种来自 Clarkia Breweri 的酶，具有花香和植物防御功能，经解析至 3.0 ¿最近，拟南芥 SABATH 家族成员 Indole-3-乙酸（生长素）羧基甲基转移酶 (IAMT) 的第一个结构已被解析为 2.85 ¿基于已解析的 Clarkia SAMT 结构，对 At 茉莉酸羧基甲基转移酶 (JMT) 的活性位点建模可以成功确定关键的 JMT 活性位点残基，并使用定点诱变来创建能够进行双功能的 SAMT。使用水杨酸和茉莉酸作为底物的 At Farnesoic 酸甲基转移酶 (FAMT) 的活性位点模型可以调节接受。我们目前的目标是对 At FAMT 和另一个 At SABATH 家族成员的结构进行表征，该成员可将赤霉酸（另一种参与植物生长和发育各个方面的植物激素）甲基化。