Adherence Assessment for Supplements

补充剂的依从性评估

基本信息

批准号：
7740800
负责人：
michael N harris
金额：
$ 37.5万
依托单位：
ICAGEN, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-30 至 2012-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7740800
关键词：
Adherence Albumins Animals Antibodies Area Authorship Award Binding Biological Biological Assay Biological Markers Blood Body Fluids Boxing Chemicals Chromium Picolinate Clinical Trials Clinical trial protocol document Collaborations Computer software Copper Crystallography Custom Data Deposition Detection Eagles Electronics Equipment Erythrocytes Exhalation External Beam Radiation Therapy Ferritin Fractionation Genetic Polymorphism Geographic Locations Haplotypes Hazardous Substances Heating Human Iron Knowledge L-Selenomethionine Laboratories Laws Lead Ligand Binding Ligands Local Government Manuscripts Measurement Measures Metabolic Metabolic Biotransformation Metabolic Pathway Metabolism Metals Methods Microscope Modality Monitor Nail plate National Center for Complementary and Alternative Medicine Noise Patients Pattern Pharmaceutical Preparations Pharmacologic Substance Phase Physiological Pilot Projects Placebos Plasma Population Preparation Proteins Proteomics Questionnaires Reaction Refuse Disposal Regulation Reporting Research Research Personnel Saliva Sampling Scientist Selenite Selenium Selenomethionine Serum Soil Solid Specimen Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectroscopy, Fourier Transform Infrared Sterilization for infection control Sweat Sweating Techniques Technology Testing Treatment Protocols Urine Vacuum Validation Variant Work Yeasts Zinc Zinc Sulfate adduct commercialization compliance behavior design diaries fast protein liquid chromatography instrument interest mass spectrometer meetings monitoring device planetary Atmosphere product development programs prototype response selenate small molecule square foot tool waterborne

项目摘要

DESCRIPTION (provided by applicant): Caldera Pharmaceuticals uses protein fractionation techniques and XRpro technology to determine protein CAM/metabolite binding as a means to determine adherence to CAM regimens in NCCAM clinical trials. Caldera will use their high-throughput proteomics technology to identify CAM Adherence Biomarkers (CABs) using our unique, national award winning XRpro technology. Caldera, in collaboration with Los Alamos National Laboratory, developed XRpro. This technology allows the identification and measurement of CAM adherence by measuring CABs consisting of protein-ligand adducts and protein-metal metabolites with no a priori knowledge of which proteins are involved. Therefore, antibodies to specific proteins or specific protein assays are not required. Caldera Pharmaceuticals has 2000 square feet of lab space and 1800 square feet of office space. This area is well equipped for chemical and biological R&D. Equipment available for this work includes: fume hoods, sterilization ovens, reaction glassware, rotary evaporator, vacuum lines for reactions and distillations, atmosphere controlled glove boxes, heating/stirring mantles, spectrometers (UV-VIS-NIR), NMR, crystallography, fluorimeters, FTIR, TGA and DSC, microscopes, a customized EDAX Eagle III MXRF spectrometer and a customized EDAX Eagle II MXRF spectrometer and Horiba XGT 5000 XRF microscope, and a custom-built confocal XRF microscope, FPLC, and an Applied Biosystems Voyager-DE STR MALDI-TOF and QSTAR XL MS/MS mass spectrometer. Caldera has access to machine and electronics shops. Caldera facilities meet environmental laws and regulations of federal, state, and local governments relating to: airborne emissions, waterborne effluents, external radiation levels, outdoor noise, solid and bulk waste disposal practices, and handling and storage of toxic and hazardous materials.

描述（由申请人提供）：Caldera Pharmaceuticals使用蛋白质分馏技术和XRPRO技术来确定蛋白质CAM/代谢物结合，作为确定NCCAM临床试验中遵守CAM方案的手段。 Caldera将使用其高通量蛋白质组学技术使用我们独特的，全国屡获殊荣的XRPRO技术来识别CAM依从性生物标志物（CABS）。 Caldera与Los Alamos国家实验室合作开发了XRPRO。这项技术允许通过测量由蛋白质 - 配体加合物和蛋白质金属代谢物组成的CAB来识别和测量CAM依从性，而没有涉及哪些蛋白质的先验知识。因此，不需要对特定蛋白质或特定蛋白质测定的抗体。 Caldera Pharmaceuticals拥有2000平方英尺的实验室空间和1800平方英尺的办公空间。该区域配备了化学和生物研发。可用于这项工作的设备包括：烟雾罩，灭菌烤箱，反应玻璃器皿，旋转蒸发器，用于反应和蒸馏的真空线，大气控制的手套箱，加热/搅拌地幔，光谱仪（UV-VIS-NIR），NMR，NMR，晶体学，荧光仪，荧光仪，荧光量, FTIR, TGA and DSC, microscopes, a customized EDAX Eagle III MXRF spectrometer and a customized EDAX Eagle II MXRF spectrometer and Horiba XGT 5000 XRF microscope, and a custom-built confocal XRF microscope, FPLC, and an Applied Biosystems Voyager-DE STR MALDI-TOF和QSTAR XL MS/MS质谱仪。 Caldera可以使用机器和电子商店。火山口设施符合与以下方面的联邦，州和地方政府的环境法规和法规：机载排放，水上流出物，外部辐射水平，室外噪音，固体和散装废物处理方式，以及对有毒和危险物质的处理和存储。