Novel Assay for Rapid Antibody Screening

快速抗体筛选的新方法

基本信息

批准号：
7676191
负责人：
Scott Allen Monsma
金额：
$ 14.55万
依托单位：
PRIMORIGEN BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7676191
关键词：
Address Affinity Antibodies Antibody Dissociations Antibody Formation Antigens Beta Cell Binding Biological Assay Cell Differentiation process Cell Extracts Cell Line Cells DNA Sequence Data Data Storage and Retrieval Deposition Detection Development Devices Differentiation Antigens Dissociation Drug Formulations Effectiveness Enzyme-Linked Immunosorbent Assay Equilibrium Evaluation Figs - dietary Funding Gel Glass Government Human Hybridomas Hydrophobic Surfaces Immunization Immunoassay Individual Insecta Insulin-Dependent Diabetes Mellitus Kinetics Left Methods Monoclonal Antibodies Mus Nature Persons Phase Phase I Clinical Trials Phase II Clinical Trials Plastics Polyesters Porosity Protein Array Protein Binding Proteins Protocols documentation Pyroxylin Reagent Reference Standards Reproducibility Research Right-On Sampling Screening procedure Slide Small Business Innovation Research Grant Solutions Specificity Speed Splenocyte Spottings Staging Structure of beta Cell of islet Surface System Systems Analysis Techniques Technology Testing Time Transplantation Western Blotting Work base cost counterscreen design expression vector high throughput analysis hybridoma production hydrophilicity improved manufacturing process miniaturize monoclonal antibody production novel research study

项目摘要

DESCRIPTION (provided by applicant): Primorigen Biosciences will use Phase I SBIR funds to develop a frameless, multiplexed, microarray screening system for rapid identification and characterization of antibody matched pairs with appropriate affinity and specificity for target proteins. This system produces improved assay content and dramatically reduces the time and cost of multiplexed microarray development. The core of this new system is Primorigen's novel frameless microarray device for multiplexed analysis that allows testing of 96 (or 384) samples against several binding targets directly on a flat array surface. As a result, dozens of proteins in hundreds of samples can be detected with high precision to generate thousands of data points per experiment, increasing speed of screening. While the new system can be adapted to detect any protein, this Phase I project will focus on detecting human pancreatic beta cell markers useful for characterizing insulin-secreting cell lines that may be candidates to treat or cure Type 1 diabetes. The Phase I project will develop a frameless nitrocellulose assay surface and use the miniaturized array methods to screen and characterize antibodies that detect the target proteins. Phase II will a) validate these assays for commercial use, b) develop manufacturing protocols, and c) apply this system to cell differentiation markers.

描述（由申请人提供）： Primorigen Biosciences 将使用 I 期 SBIR 资金开发无框架、多重微阵列筛选系统，用于快速鉴定和表征对靶蛋白具有适当亲和力和特异性的抗体匹配对。该系统可产生改进的检测内容，并显着减少多重微阵列开发的时间和成本。这个新系统的核心是 Primorigen 的新型无框架微阵列设备，用于多重分析，允许直接在平面阵列表面上针对多个结合靶点测试 96 个（或 384 个）样品。因此，可以高精度检测数百个样品中的数十种蛋白质，从而在每个实验中生成数千个数据点，从而提高筛选速度。虽然新系统可以适用于检测任何蛋白质，但该一期项目将重点检测人类胰腺 β 细胞标记物，这些标记物可用于表征胰岛素分泌细胞系，这些细胞系可能是治疗或治愈 1 型糖尿病的候选细胞。第一阶段项目将开发无框硝化纤维检测表面，并使用小型化阵列方法来筛选和表征检测目标蛋白的抗体。第二阶段将a）验证这些测定的商业用途，b）开发生产方案，以及c）将该系统应用于细胞分化标记。